A miniaturized microtiter plate protocol for the determination of selenomethionine in selenized yeast via enzymatic hydrolysis of protein-bound selenium

2011 ◽  
Vol 3 (3) ◽  
pp. 738 ◽  
Author(s):  
Michael Stiboller ◽  
Markus Damm ◽  
Allycia M. Barbera ◽  
Doris Kuehnelt ◽  
Kevin A. Francesconi ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Microtiter Plate ◽  
Hydrolysis Of ◽  
Selenized Yeast

The determination of urea in soil extracts and related samples—a review

Soil Research ◽  
2004 ◽  
Vol 42 (7) ◽  
pp. 709 ◽  
Author(s):  
David F. Lambert ◽  
John E. Sherwood ◽  
Paul S. Francis
Keyword(s):  
Enzymatic Hydrolysis ◽  
Flow Injection Analysis ◽  
Acidic Solution ◽  
Clinical Applications ◽  
Urease Inhibitors ◽  
Viable Option ◽  
Soil Extracts ◽  
Diacetyl Monoxime ◽  
Hydrolysis Of

Although the dominant methods for the determination of urea in clinical applications incorporate selective enzymatic hydrolysis of urea, the determination of urea in soil extracts is complicated by the presence of urease inhibitors. The spectrophotometric determination of urea with an acidic solution diacetyl monoxime and semicarbazide is a viable option but traditional manual procedures are time-consuming. New variations on these procedures, based on microplates or flow-injection analysis methodologies, allow a far greater number of samples to be analysed with high precision and sensitivity.

scholarly journals Determination of inhibition in the enzymatic hydrolysis of cellobiose using hybrid neural modeling

2005 ◽  
Vol 22 (1) ◽  
pp. 19-29 ◽  
Author(s):  
F. C. Corazza ◽  
L. P. V. Calsavara ◽  
F. F. Moraes ◽  
G. M. Zanin ◽  
I. Neitzel
Keyword(s):  
Enzymatic Hydrolysis ◽  
Neural Modeling ◽  
Hydrolysis Of

Selecting the right blood glucose monitor for the determination of glucose during the enzymatic hydrolysis of corncob pretreated with different methods

2011 ◽  
Vol 102 (20) ◽  
pp. 9646-9652 ◽  
Author(s):  
Erinc Bahcegul ◽  
Emre Tatli ◽  
Nazife Isik Haykir ◽  
Serpil Apaydin ◽  
Ufuk Bakir
Keyword(s):  
Blood Glucose ◽  
Enzymatic Hydrolysis ◽  
Blood Glucose Monitor ◽  
The Right ◽  
Hydrolysis Of

Sequence Determination of an Antioxidant Peptide Obtained by Enzymatic Hydrolysis of Oyster Crassostrea madrasensis (Preston)

2016 ◽  
Vol 22 (3) ◽  
pp. 421-433 ◽  
Author(s):  
K. K. Asha ◽  
K. R. Remya Kumari ◽  
K. Ashok Kumar ◽  
Niladri S. Chatterjee ◽  
R. Anandan ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sequence Determination ◽  
Antioxidant Peptide ◽  
Hydrolysis Of

scholarly journals Determination of Opiates in Whole Blood and Vitreous Humor: A Study of the Matrix Effect and an Experimental Design to Optimize Conditions for the Enzymatic Hydrolysis of Glucuronides

2012 ◽  
Vol 36 (3) ◽  
pp. 162-170 ◽  
Author(s):  
L. R. Sanches ◽  
S. C. Seulin ◽  
V. Leyton ◽  
B. A. P. Bismara Paranhos ◽  
C. A. Pasqualucci ◽  
...  
Keyword(s):  
Experimental Design ◽  
Enzymatic Hydrolysis ◽  
Matrix Effect ◽  
Whole Blood ◽  
Vitreous Humor ◽  
The Matrix ◽  
Hydrolysis Of

scholarly journals The enzymatic method for the quantitative determination of benzalkonium chloride in the antiseptic solution “CUTASEPT® F”

2021 ◽  
Vol 19 (4(76)) ◽  
pp. 33-39
Author(s):  
Olena V. Koval’ska ◽  
Mykola Ye. Blazheyevskіy
Keyword(s):  
Enzymatic Hydrolysis ◽  
Quantitative Determination ◽  
Peracetic Acid ◽  
Benzalkonium Chloride ◽  
Chloride Content ◽  
Inhibitory Effect ◽  
Enzymatic Method ◽  
Limit Of Quantitation ◽  
Hydrolysis Of

Aim. To develop an alternative method for the quantitative determination of the benzalkonium chloride content as an active pharmaceutical ingredient in the disinfectant solution “CUTASEPT® F”.Materials and methods. The method is based on the ability of benzalkonium chloride to inhibit the enzymatic hydrolysis of acetylcholine by acetylcholinesterase. The reaction rate is assessed by the non-hydrolyzed acetylcholine residue, which is determined by the amount of peracetic acid produced during the interaction with the excess of the hydrogen peroxide solution. The indicator reaction is the interaction of p-phenetidine with peracetic acid that leads to the formation of 4,4’-azoxyphenetole with λmax = 358 nm (log10 ε = 4.2).Results and discussion. As a result of the research conducted the linear dependence of the degree of inhibition of the enzymatic hydrolysis of acetylcholine (U, %) on the concentration of benzalkonium chloride was determined in the concentration range of (0.5 – 7.0) × 10–6 mol L-1 with the correlation coefficient of 0.999. The limit of quantitation was 1.9 × 10–6 mol L-1.Conclusions. As a result of the research conducted the kinetic enzymatic method for the quantitative determination of benzalkonium chloride has been developed by its inhibitory effect in the biochemical reaction of acetylcholine hydrolysis. This method is fast, cheap and easy to perform, does not require expensive equipment, and available for use in the field.

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