Simple, rapid and label-free colorimetric assay for Zn2+ based on unmodified gold nanoparticles and specific Zn2+ binding peptide

2011 ◽  
Vol 47 (15) ◽  
pp. 4412 ◽  
Author(s):  
Wang Li ◽  
Zhou Nie ◽  
Kaiyu He ◽  
Xiahong Xu ◽  
Yong Li ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Assay ◽  
Label Free ◽  
Binding Peptide

scholarly journals Development of Label-Free Colorimetric Assay for MERS-CoV Using Gold Nanoparticles

ACS Sensors ◽  
2019 ◽  
Vol 4 (5) ◽  
pp. 1306-1312 ◽  
Author(s):  
Hanbi Kim ◽  
Minseon Park ◽  
Joonki Hwang ◽  
Jin Hwa Kim ◽  
Doo-Ryeon Chung ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Assay ◽  
Label Free

scholarly journals Correction to Development of Label-Free Colorimetric Assay for MERS-CoV Using Gold Nanoparticles

ACS Sensors ◽  
2019 ◽  
Vol 4 (9) ◽  
pp. 2554-2554 ◽  
Author(s):  
Hanbi Kim ◽  
Minseon Park ◽  
Joonki Hwang ◽  
Jin Hwa Kim ◽  
Doo-Ryeon Chung ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Assay ◽  
Label Free

scholarly journals Specific pH effect for selective colorimetric assay of glutathione using anti-aggregation of label-free gold nanoparticles

RSC Advances ◽  
2017 ◽  
Vol 7 (22) ◽  
pp. 13426-13432 ◽  
Author(s):  
Jian-Fang Li ◽  
Peng-Cheng Huang ◽  
Fang-Ying Wu
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Assay ◽  
Ph Effect ◽  
Colorimetric Method ◽  
Label Free ◽  
Free Gold

An operationally simple colorimetric method for measuring glutathione (GSH) concentration was developed using anti-aggregation of gold nanoparticles (AuNPs) in this work.

A colorimetric approach for measuring mercuric ions with high selectivity using label-free gold nanoparticles and thiourea

2015 ◽  
Vol 7 (16) ◽  
pp. 6837-6841 ◽  
Author(s):  
Qianru Wang ◽  
Yiran Li ◽  
Min Li ◽  
Congying Wen ◽  
Rongsheng Liu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
High Selectivity ◽  
Colorimetric Assay ◽  
Label Free ◽  
Au Nps ◽  
Free Gold ◽  
Mercuric Ions

A simple, sensitive and selective colorimetric assay was developed for detecting Hg2+ by coupling Au NPs with thiourea.

scholarly journals Development of label-free gold nanoparticle based rapid colorimetric assay for clinical/point-of-care screening of cervical cancer

2020 ◽  
Vol 2 (12) ◽  
pp. 5737-5745
Author(s):  
Tejaswini Appidi ◽  
Sushma V. Mudigunda ◽  
Suseela Kodandapani ◽  
Aravind Kumar Rengan
Keyword(s):  
Cervical Cancer ◽  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Colorimetric Assay ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Label Free ◽  
Free Gold ◽  
Clinical Point

“C-ColAur” technique for colorimetric detection of cervical cancer by in situ formation of gold nanoparticles.

Gold nanoparticle-based exonuclease III signal amplification for highly sensitive colorimetric detection of folate receptor

Nanoscale ◽  
2014 ◽  
Vol 6 (6) ◽  
pp. 3055-3058 ◽  
Author(s):  
Xinjian Yang ◽  
Zhiqiang Gao
Keyword(s):  
Gold Nanoparticles ◽  
Small Molecule ◽  
Signal Amplification ◽  
Colorimetric Assay ◽  
Folate Receptor ◽  
Colorimetric Detection ◽  
Label Free ◽  
Exonuclease Iii ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

By combining terminal protection of small molecule (folate)-capped DNA probes, exonuclease III signal amplification and gold nanoparticles, we developed a simple and label-free colorimetric assay for highly sensitive detection of folate receptor.

scholarly journals Detection of Sub-Nanomolar Concentration of Trypsin by Thickness-Shear Mode Acoustic Biosensor and Spectrophotometry

Biosensors ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 117
Author(s):  
Ivan Piovarci ◽  
Sopio Melikishvili ◽  
Marek Tatarko ◽  
Tibor Hianik ◽  
Michael Thompson
Keyword(s):  
Gold Nanoparticles ◽  
Protease Activity ◽  
Limit Of Detection ◽  
Colorimetric Assay ◽  
Enzymatic Reaction ◽  
Disease Diagnosis ◽  
Shear Mode ◽  
Label Free ◽  
Thickness Shear Mode ◽  
Thickness Shear

The determination of protease activity is very important for disease diagnosis, drug development, and quality and safety assurance for dairy products. Therefore, the development of low-cost and sensitive methods for assessing protease activity is crucial. We report two approaches for monitoring protease activity: in a volume and at surface, via colorimetric and acoustic wave-based biosensors operated in the thickness-shear mode (TSM), respectively. The TSM sensor was based on a β-casein substrate immobilized on a piezoelectric quartz crystal transducer. After an enzymatic reaction with trypsin, it cleaved the surface-bound β-casein, which increased the resonant frequency of the crystal. The limit of detection (LOD) was 0.48 ± 0.08 nM. A label-free colorimetric assay for trypsin detection has also been performed using β-casein and 6-mercaptohexanol (MCH) functionalized gold nanoparticles (AuNPs/MCH-β-casein). Due to the trypsin cleavage of β-casein, the gold nanoparticles lost shelter, and MCH increased the attractive force between the modified AuNPs. Consequently, AuNPs aggregated, and the red shift of the absorption spectra was observed. Spectrophotometric assay enabled an LOD of 0.42 ± 0.03 nM. The Michaelis–Menten constant, KM, for reverse enzyme reaction has also been estimated by both methods. This value for the colorimetric assay (0.56 ± 0.10 nM) is lower in comparison with those for the TSM sensor (0.92 ± 0.44 nM). This is likely due to the better access of the trypsin to the β-casein substrate at the surface of AuNPs in comparison with those at the TSM transducer.

Simple, rapid and label-free colorimetric assay for arsenic based on unmodified gold nanoparticles and a phytochelatin-like peptide

2012 ◽  
Vol 4 (12) ◽  
pp. 3937 ◽  
Author(s):  
Ning Xia ◽  
Yunfeng Shi ◽  
Renchun Zhang ◽  
Feng Zhao ◽  
Feng Liu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Assay ◽  
Label Free

Fluorosurfactant-capped gold nanoparticles-based label-free colorimetric assay for Au3+ with tunable dynamic range via a redox strategy

2013 ◽  
Vol 48 ◽  
pp. 1-5 ◽  
Author(s):  
Bin Yang ◽  
Xiao-Bing Zhang ◽  
Wei-Na Liu ◽  
Rong Hu ◽  
Weihong Tan ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dynamic Range ◽  
Colorimetric Assay ◽  
Label Free
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