Gold nanoparticle-based exonuclease III signal amplification for highly sensitive colorimetric detection of folate receptor

By combining terminal protection of small molecule (folate)-capped DNA probes, exonuclease III signal amplification and gold nanoparticles, we developed a simple and label-free colorimetric assay for highly sensitive detection of folate receptor.

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Terminal protection of small molecule-linked ssDNA for label-free and highly sensitive colorimetric detection of folate receptor biomarkers

RSC Advances ◽

10.1039/c4ra13997f ◽

2015 ◽

Vol 5 (8) ◽

pp. 6100-6105 ◽

Cited By ~ 7

Author(s):

Xue Gong ◽

Wenjiao Zhou ◽

Yaqin Chai ◽

Yun Xiang ◽

Ruo Yuan

Keyword(s):

Small Molecule ◽

Folate Receptor ◽

Colorimetric Detection ◽

Sensitive Detection ◽

Label Free ◽

Folate Receptors ◽

Color Transition ◽

Highly Sensitive

Protection of ssDNA from digesting by Exo I generates amplified color transition for label-free and sensitive detection of folate receptors.

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Zwitterionic peptide-capped gold nanoparticles for colorimetric detection of Ni2+

Nanoscale ◽

10.1039/c7nr07998b ◽

2018 ◽

Vol 10 (12) ◽

pp. 5466-5473 ◽

Cited By ~ 12

Author(s):

Attasith Parnsubsakul ◽

Sukunya Oaew ◽

Werasak Surareungchai

Keyword(s):

Gold Nanoparticles ◽

Colorimetric Detection ◽

Sensitive Detection ◽

Highly Sensitive ◽

Highly Sensitive Detection

Recyclable zwitterionic peptide-capped AuNPs for highly sensitive detection of Ni2+.

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Optimization of the Electrodeposition of Gold Nanoparticles for the Application of Highly Sensitive, Label-Free Biosensor

Biosensors ◽

10.3390/bios9020050 ◽

2019 ◽

Vol 9 (2) ◽

pp. 50 ◽

Cited By ~ 3

Author(s):

Hao-Chun Chiang ◽

Yanyan Wang ◽

Qi Zhang ◽

Kalle Levon

Keyword(s):

Gold Nanoparticles ◽

Signal Amplification ◽

Electrochemical Biosensor ◽

Aqueous Electrolyte ◽

Label Free ◽

Reduction Step ◽

Glucose Biosensors ◽

Highly Sensitive ◽

Amperometric Glucose Biosensors ◽

Aunp Surface

A highly sensitive electrochemical biosensor with a signal amplification platform of electrodeposited gold nanoparticle (AuNP) has been developed and characterized. The sizes of the synthesized AuNP were found to be critical for the performance of biosensor in which the sizes were dependent on HAuCl4 and acid concentrations; as well as on scan cycles and scan rates in the gold electro-reduction step. Systematic investigations of the adsorption of proteins with different sizes from aqueous electrolyte solution onto the electrodeposited AuNP surface were performed with a potentiometric method and calibrated by design of experiment (DOE). The resulting amperometric glucose biosensors was demonstrated to have a low detection limit (> 50 μM) and a wide linear range after optimization with AuNP electrodeposition.

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A label-free protamine-assisted colorimetric sensor for highly sensitive detection of S1 nuclease activity

The Analyst ◽

10.1039/d0an00060d ◽

2020 ◽

Vol 145 (7) ◽

pp. 2774-2778

Author(s):

Jiahui Wen ◽

Yongming Liu ◽

Jingwen Li ◽

Hao Lin ◽

Yiran Zheng ◽

...

Keyword(s):

Gold Nanoparticles ◽

Colorimetric Method ◽

Nuclease Activity ◽

Colorimetric Sensor ◽

Sensitive Detection ◽

Label Free ◽

S1 Nuclease ◽

Highly Sensitive ◽

Highly Sensitive Detection

A label-free, simple and rapid colorimetric method for the sensitive detection of S1 nuclease activity based on protamine-assisted aggregation of gold nanoparticles.

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Highly sensitive detection of nucleic acids using a cascade amplification strategy based on exonuclease III-assisted target recycling and conjugated polyelectrolytes

The Analyst ◽

10.1039/c8an01024b ◽

2018 ◽

Vol 143 (18) ◽

pp. 4267-4272 ◽

Cited By ~ 6

Author(s):

Biqing Bao ◽

Yanrui Pan ◽

Bingbing Gu ◽

Jia Chen ◽

Yu Xu ◽

...

Keyword(s):

Nucleic Acids ◽

Signal Amplification ◽

Conjugated Polyelectrolytes ◽

Exonuclease Iii ◽

Target Recycling ◽

Highly Sensitive ◽

Exo Iii ◽

Highly Sensitive Detection ◽

Amplification Strategy ◽

Cascade Amplification

A ratiometric and cascade amplification strategy that combines the signal amplification and effecitive FRET property of CPEs with the Exo III-assisted target recycling method has been developed for DNA detection.

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