Synthesis, characterization, DNA interaction and potential applications of gold nanoparticles functionalized with Acridine Orange fluorophores

Nucleic acid is the key unit and a predominant genetic material for interpreting the fundamental basis of genetic information in an organism and now it is used for the evolution of a novel group of therapeutics. To identify the potential impact on the biological science, it receives high recognition in therapeutic applications. Due to its selective recognition of molecular targets and pathways, DNA significantly imparts tremendous specificity of action. Examining the properties of DNA holds numerous advantages in assembly, interconnects, computational elements, along with potential applications of DNA self-assembly and scaffolding include nanoelectronics, biosensors, and programmable/autonomous molecular machines. The interaction of low molecular weight, small molecules with DNA is a significant feature in pharmacology. Based on the mode of binding mechanisms, small molecules are categorized as intercalators and groove binders having a significant role in target-based drug development. The understanding mechanism of drug-DNA interaction plays an important role in the development of novel drug molecules with more effective and lesser side effects. This article attempts to outline those interactions of drug-DNA with both experimental and computational advances, including ultraviolet (UV) -visible spectroscopy, fluorescent spectroscopy, circular dichroism, nuclear magnetic resonance (NMR), molecular docking and dynamics, and quantum mechanical applications.

Download Full-text

Biosynthesis and Potential Applications of Silver and Gold Nanoparticles and Their Chitosan-Based Nanocomposites in Nanomedicine

Journal of Nanotechnology ◽

10.1155/2018/4290705 ◽

2018 ◽

Vol 2018 ◽

pp. 1-13 ◽

Cited By ~ 10

Author(s):

Haliza Katas ◽

Noor Zianah Moden ◽

Chei Sin Lim ◽

Terence Celesistinus ◽

Jie Yee Chan ◽

...

Keyword(s):

Gold Nanoparticles ◽

Metallic Nanoparticles ◽

Cost Effective ◽

One Pot ◽

Silver And Gold Nanoparticles ◽

Stabilizing Agents ◽

Cost Effective Method ◽

Multistep Process ◽

Potential Applications ◽

Biological Sources

Biosynthesized or biogenic metallic nanoparticles, particularly silver and gold nanoparticles (AgNPs and AuNPs, respectively), have been increasingly used because of their advantages, including high stability and loading capacity; moreover, these nanoparticles are synthesized using a green and cost-effective method. Previous studies have investigated reducing and/or stabilizing agents from various biological sources, including plants, microorganisms, and marine-derived products, using either a one-pot or a multistep process at different conditions. In addition, extensive studies have been performed to determine the biological or pharmacological effects of these nanoparticles, such as antimicrobial, antitumor, anti-inflammatory, and antioxidant effects. In the recent years, chitosan, a natural cationic polysaccharide, has been increasingly investigated as a reducing and/or stabilizing agent in the synthesis of biogenic metallic nanoparticles with potential applications in nanomedicine. Here, we have reviewed the mechanism of biosynthesis and potential applications of AgNPs and AuNPs and their chitosan-mediated nanocomposites in nanomedicine.

Download Full-text

Cancer theranosis using mono-disperse, mesoporous gold nanoparticles obtained via a robust, high-yield synthetic methodology

RSC Advances ◽

10.1039/c5ra24772a ◽

2016 ◽

Vol 6 (16) ◽

pp. 13554-13561 ◽

Cited By ~ 8

Author(s):

Taeksu Lee ◽

Doyeon Bang ◽

Yong Wook Chang ◽

Yuna Choi ◽

Kwang Yeol Park ◽

...

Keyword(s):

Gold Nanoparticles ◽

High Yield ◽

Synthetic Methodology ◽

Facile Synthesis ◽

Potential Applications

Here, we introduce the facile synthesis of scalable, mono-disperse, mesoporous gold nanoparticles (MPGNs) with an acidic emulsification method, which exhibit many attractive nanoplasmonic features for potential applications in many fields.

Download Full-text

Elucidation of photophysical changes and orientation of acridine orange dye on the surface of borate capped gold nanoparticles using multi-spectroscopic techniques

Photochemical & Photobiological Sciences ◽

10.1039/c4pp00223g ◽

2014 ◽

Vol 13 (12) ◽

pp. 1741-1752 ◽

Cited By ~ 17

Author(s):

Arumugam Selva Sharma ◽

Malaichamy Ilanchelian

Keyword(s):

Gold Nanoparticles ◽

Acridine Orange ◽

Spectroscopic Techniques ◽

Binding Interaction ◽

Au Nps

In the present work, we have carried out a detailed investigation on the binding interaction of acridine orange (AO) with borate capped gold nanoparticles (Au NPs) by multi spectroscopic techniques.

Download Full-text

Chapter 5. Glyconanotechnology and Disease: Gold Nanoparticles Coated with Glycosides as Multivalent Systems for Potential Applications in Diagnostics and Therapy

Drug Discovery - Carbohydrates in Drug Design and Discovery ◽

10.1039/9781849739993-00089 ◽

2015 ◽

pp. 89-131 ◽

Cited By ~ 1

Author(s):

Marco Marradi ◽

Fabrizio Chiodo ◽

Isabel García

Keyword(s):

Gold Nanoparticles ◽

Potential Applications ◽

Diagnostics And Therapy

Download Full-text

Nanodiamonds and gold nanoparticles to obtain a hybrid nanostructure with potential applications in biomedicine

Nanotechnology ◽

10.1088/1361-6528/aadad1 ◽

2018 ◽

Vol 29 (43) ◽

pp. 435101 ◽

Cited By ~ 2

Author(s):

Seidy Pedroso-Santana ◽

Noralvis Fleitas-Salazar ◽

Andrei Sarabia-Sainz ◽

Erika Silva-Campa ◽

Araceli Angulo-Molina ◽

...

Keyword(s):

Gold Nanoparticles ◽

Hybrid Nanostructure ◽

Potential Applications

Download Full-text

Laser-controlled precipitation of gold nanoparticles in silicate glasses

Journal of Materials Research ◽

10.1557/jmr.2003.0294 ◽

2003 ◽

Vol 18 (9) ◽

pp. 2097-2100 ◽

Cited By ~ 5

Author(s):

Xiongwei Jiang ◽

Jianrong Qiu ◽

Huidan Zeng ◽

Congshan Zhu

Keyword(s):

Heat Treatment ◽

Gold Nanoparticles ◽

Femtosecond Laser ◽

Optical Switches ◽

Sample Heat ◽

Selective Precipitation ◽

Heat Treated ◽

All Optical ◽

Potential Applications ◽

Controlled Precipitation

We report on the observation of space-selective precipitation of gold nanoparticles in Au2O-doped silicate glass by a method of irradiation with an 800-nm femtosecond laser and further heat treatment. The irradiated region of the glass first became gray in color after irradiation with the femtosecond laser and then turned red after further heat treatment at around 520 °C, indicating that gold nanoparticles have precipitated in the irradiated region of the glass. A possible mechanism has been suggested that the Au+ ions in the region irradiated are reduced to Au0 atoms by the femtosecond laser, and then the Au0 atoms accumulate to form gold nanoparticles with the glass sample heat treated. The observed phenomenon should have potential applications in the fabrication of ultrafast all-optical switches.

Download Full-text