Magnetic CoPt nanoparticles as MRI contrast agent for transplanted neural stem cells detection

Nanoscale ◽  
2011 ◽  
Vol 3 (3) ◽  
pp. 977 ◽  
Author(s):  
Xiaoting Meng ◽  
Hugh C. Seton ◽  
Le T. Lu ◽  
Ian A. Prior ◽  
Nguyen T. K. Thanh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Contrast Agent ◽  
Mri Contrast Agent ◽  
Mri Contrast

Abstract 403: Tracking the Migration of Porcine Mesenchymal Stem Cells with the MRI Contrast Agent Ferex in an AAA Model

2012 ◽  
Vol 32 (suppl_1) ◽  
Author(s):  
Rami Tadros ◽  
Bhakti Rawal ◽  
Karen Briley-Saebo ◽  
David O’Connor ◽  
Dan Han ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Contrast Agent ◽  
Lentiviral Vector ◽  
Relaxation Times ◽  
Mri Contrast Agent ◽  
Signal Loss ◽  
Post Transplantation ◽  
Mri Contrast

Introduction: Mesenchymal stem cells (MSC) are being investigated in porcine abdominal aortic aneurysm (PAAA) models for their repair potential. This study uses MSCs labeled with the MRI contrast agent Ferex to non-invasively evaluate MSC migration in-vivo. Methods: MSCs from 6 pigs were isolated from bone marrow via Ficoll Paque separation and expanded in culture. Using a Lentiviral vector, MSC from all 6 pigs were transfected with green florescent protein (GFP). MSCs from 4 of these pigs were also labeled with 200μg/ml Ferex using Poly-L-Lysine and then analyzed for Ferex uptake and viability. Preservation of the MSC phenotype was confirmed using flow cytometry by detecting positive CD90 and negative CD45 and CD117. Transmission electron microscopy established that Ferex localized to lysosomes. MSCs were then injected into the adventitia of the PAAA. In-vivo MRI was performed using multiple echo gradient echo sequences. Effective transverse relaxation times (T2* values) were calculated on a pixel-by-pixel basis as a function of time post cell transplantation. Results: Ferex labeled MSCs were visible post transplantation at 4, 11, 15 and 21 days using MRI. The MRI signal void (decreased T2* values) correlated with the presence of Ferex within the PAAA. This signal loss progressively expanded circumferentially at each study interval representing cellular movement. MSC migration and localization were confirmed with GFP visualization on fluorescence microscopy and immunohistochemistry. In-vivo MRI signals also correlate with iron deposition on Perl’s stain. Conclusion: Ferex can be used as an in-vivo tracking agent of MSCs in PAAA models.

scholarly journals Aptamer-PEG-modified Fe3O4@Mn as a novel T1- and T2- dual-model MRI contrast agent targeting hypoxia-induced cancer stem cells

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Haitao Zhu ◽  
Lirong Zhang ◽  
Yanfang Liu ◽  
Yuepeng Zhou ◽  
Kang Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Contrast Agent ◽  
Mri Contrast Agent ◽  
Dual Model ◽  
Mri Contrast ◽  
T1 And T2

scholarly journals PS196. Tracking the Migration of Porcine Mesenchymal Stem Cells with the MRI Contrast Agent Ferex in an AAA Model

2012 ◽  
Vol 55 (6) ◽  
pp. 76S-77S
Author(s):  
Rami Tadros ◽  
Bhakti Rawal ◽  
Karen Briley-Saebo ◽  
David O'Connor ◽  
Dan Han ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Contrast Agent ◽  
Mri Contrast Agent ◽  
Mri Contrast

Successful labeling of porcine mesenchymal stem cells with MRI contrast agent Ferex for use in AAA models

2011 ◽  
Vol 213 (3) ◽  
pp. S154-S155
Author(s):  
Rami Tadros ◽  
Dan Han ◽  
Karen Briley-Saebo ◽  
Mernoosh Shahrivar ◽  
Andrew Tye ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Contrast Agent ◽  
Mri Contrast Agent ◽  
Mri Contrast

scholarly journals Use of trimetasphere metallofullerene MRI contrast agent for the non-invasive longitudinal tracking of stem cells in the lung

Methods ◽  
2016 ◽  
Vol 99 ◽  
pp. 99-111 ◽  
Author(s):  
Sean V. Murphy ◽  
Austin Hale ◽  
Tanya Reid ◽  
John Olson ◽  
Amritha Kidiyoor ◽  
...  
Keyword(s):  
Stem Cells ◽  
Contrast Agent ◽  
Mri Contrast Agent ◽  
Mri Contrast ◽  
Non Invasive ◽  
Longitudinal Tracking

scholarly journals D-mannose-Coating of Maghemite Nanoparticles Improved Labeling of Neural Stem Cells and Allowed Their Visualization by ex vivo MRI after Transplantation in the Mouse Brain

2019 ◽  
Vol 28 (5) ◽  
pp. 553-567 ◽  
Author(s):  
Igor M. Pongrac ◽  
Marina Dobrivojević Radmilović ◽  
Lada Brkić Ahmed ◽  
Hrvoje Mlinarić ◽  
Jan Regul ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Mouse Brain ◽  
Ex Vivo ◽  
Superparamagnetic Iron Oxide ◽  
Mri Contrast Agent ◽  
Invasive Technique ◽  
Maghemite Nanoparticles ◽  
Mri Contrast ◽  
Magnetic Resonance Imaging Mri

Magnetic resonance imaging (MRI) of superparamagnetic iron oxide-labeled cells can be used as a non-invasive technique to track stem cells after transplantation. The aim of this study was to (1) evaluate labeling efficiency of D-mannose-coated maghemite nanoparticles (D-mannose(γ-Fe2O3)) in neural stem cells (NSCs) in comparison to the uncoated nanoparticles, (2) assess nanoparticle utilization as MRI contrast agent to visualize NSCs transplanted into the mouse brain, and (3) test nanoparticle biocompatibility. D-mannose(γ-Fe2O3) labeled the NSCs better than the uncoated nanoparticles. The labeled cells were visualized by ex vivo MRI and their localization subsequently confirmed on histological sections. Although the progenitor properties and differentiation of the NSCs were not affected by labeling, subtle effects on stem cells could be detected depending on dose increase, including changes in cell proliferation, viability, and neurosphere diameter. D-mannose coating of maghemite nanoparticles improved NSC labeling and allowed for NSC tracking by ex vivo MRI in the mouse brain, but further analysis of the eventual side effects might be necessary before translation to the clinic.

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