One-pot preparation of polyethylenimine-silica nanoparticles as serum-resistant gene delivery vectors: Intracellular trafficking and transfection

2011 ◽  
Vol 21 (28) ◽  
pp. 10496 ◽  
Author(s):  
Wen-Tao He ◽  
Ya-Nan Xue ◽  
Na Peng ◽  
Wen-Ming Liu ◽  
Ren-Xi Zhuo ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Silica Nanoparticles ◽  
Intracellular Trafficking ◽  
One Pot ◽  
Resistant Gene ◽  
Gene Delivery Vectors

On the role of the colloidal stability of mesoporous silica nanoparticles as gene delivery vectors

2011 ◽  
Vol 13 (9) ◽  
pp. 4097-4108 ◽  
Author(s):  
Virginia Cebrián ◽  
Clara Yagüe ◽  
Manuel Arruebo ◽  
Francisco M. Martín-Saavedra ◽  
Jesus Santamaría ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Colloidal Stability ◽  
Mesoporous Silica Nanoparticles ◽  
Gene Delivery Vectors

Multi-armed poly(l-glutamic acid)-graft-polypropyleneinime as effective and serum resistant gene delivery vectors

2014 ◽  
Vol 465 (1-2) ◽  
pp. 444-454 ◽  
Author(s):  
Xuan Zhang ◽  
Haimei Hu ◽  
Tiebang Liu ◽  
Yeya Yang ◽  
Yuefeng Peng ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Glutamic Acid ◽  
Resistant Gene ◽  
Gene Delivery Vectors

scholarly journals Intracellular trafficking of hybrid gene delivery vectors

2015 ◽  
Vol 207 ◽  
pp. 120-130 ◽  
Author(s):  
Rahul K. Keswani ◽  
Mihael Lazebnik ◽  
Daniel W. Pack
Keyword(s):  
Gene Delivery ◽  
Intracellular Trafficking ◽  
Hybrid Gene ◽  
Gene Delivery Vectors

Recent advances in the analysis full/empty capsid ratio and genome integrity of adeno-associated virus (AAV) gene delivery vectors

2020 ◽  
Vol 20 ◽  
Author(s):  
L. Hajba ◽  
A. Guttman
Keyword(s):  
Gene Delivery ◽  
High Efficiency ◽  
Analytical Techniques ◽  
Viral Gene ◽  
Adeno Associated Virus ◽  
Gene Delivery Vectors ◽  
Empty Capsid ◽  
Purification Methods ◽  
Viral Gene Delivery

: Adeno-associated virus (AAV) is one of the most promising viral gene delivery vectors with long-term gene expression and disease correction featuring high efficiency and excellent safety in human clinical trials. During the production of AAV vectors,there are several quality control (QC)parameters that should be rigorously monitored to comply with clini-cal safety and efficacy. This review gives a short summary of the most frequently used AVV production and purification methods,focusing on the analytical techniques applied to determine the full/empty capsid ratio and the integrity of the encapsidated therapeutic DNA of the products.

scholarly journals Glucose-Responsive Gene Delivery at Physiological pH through Tertiary-Amine Stabilized Boronate-PVA Particles Synthesized by One-Pot Reaction

Pharmaceutics ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 62
Author(s):  
Mangesh Morey ◽  
Akshay Srivastava ◽  
Abhay Pandit
Keyword(s):  
Gene Delivery ◽  
Fold Increase ◽  
Nonviral Gene Delivery ◽  
Luciferase Reporter ◽  
Hydrodynamic Diameter ◽  
Luciferase Reporter Gene ◽  
One Pot ◽  
Gaussia Luciferase ◽  
Nih3t3 Cells ◽  
The Stability

We report a physiologically stable and cytocompatible glucose-responsive nonviral gene delivery system made up of boronate functionalized polymeric material. Herein, we utilize boronate cis-diol interactions to develop a glucose-responsive submicron particle (SMP) system. The stability of the boronate interaction at a physiological pH was achieved by copolymerization of dimethyl aminoethyl methacrylate (DMAEMA) with acrylamidophenylboronic acid (AAPBA) and the formation of a complex with polyvinylalcohol (PVA) which is governed by cis-diol interactions. The shift in hydrodynamic diameter of SMPs was observed and correlated with increasing glucose concentrations at a physiological pH. Optimal transfection was observed for a 5 µg dose of the gaussia luciferase reporter gene in NIH3T3 cells without any adverse effect on cellular viability. The destabilization of the AAPBA–PVA complex by interacting with glucose allowed the release of encapsulated bovine serum albumin (BSA) in a glucose-responsive manner. In total, 95% of BSA was released from SMPs at a 50 mM glucose concentration after 72 h. A two-fold increase in transfection was observed in 50 mM glucose compared to that of 10 mM glucose.

pH-sensitive N,N-(dimethyl)-N-alkanamine-N-oxides as gene delivery vectors

2017 ◽  
Vol 71 (9) ◽  
pp. 1739-1748 ◽  
Author(s):  
Gilda Liskayová ◽  
Lukáš Hubčík ◽  
Katarína Šišková ◽  
Ingrid Pauliková ◽  
Dominika Galliková ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Ph Sensitive ◽  
Gene Delivery Vectors

One-pot synthesis and application of novel amino-functionalized silica nanoparticles using guanidine as amino group

2016 ◽  
Vol 40 (10) ◽  
pp. 8444-8450 ◽  
Author(s):  
Feng Guodong ◽  
Guan Mingming ◽  
Lai Qi ◽  
Mi Hongyu ◽  
Li Guanghua ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Silica Nanoparticles ◽  
Amino Group ◽  
Functionalized Silica ◽  
Henry Reaction ◽  
Modified Silica ◽  
One Pot ◽  
One Pot Synthesis ◽  
Modified Silica Nanoparticles ◽  
Functionalized Silica Nanoparticles

The preparation of modified silica nanoparticles with guanidine was developed and used to catalyze the Henry reaction and fix quantum dots.

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