Escherichia coli bacteria were determined by in flow cytometry with laser excitation and fluorescence detection applying ultraluminescent core–shell nanoparticles based on Metal Enhanced Fluorescence (MEF).
Core–shell nanoparticles are versatile nanostructures that can be applied as luminescent biosensing platforms in many nanotechnological developments.
The metal-enhanced fluorescence of Au25-adsorbed Ag@SiO2 nanoparticles is due to the increase of the radiative decay rate constant.
Tetracycline (Tc) selectively binds with europium (Eu3+) which emits red fluorescence (615 nm) upon excitation at 400 nm.