Radiolabeled Gold Nanoseeds Decorated with Substance P Peptides: Synthesis, Characterization and In Vitro Evaluation in Glioblastoma Cellular Models

Despite some progress, the overall survival of patients with glioblastoma (GBM) remains extremely poor. In this context, there is a pressing need to develop innovative therapy strategies for GBM, namely those based on nanomedicine approaches. Towards this goal, we have focused on nanoparticles (AuNP-SP and AuNP-SPTyr8) with a small gold core (ca. 4 nm), carrying DOTA chelators and substance P (SP) peptides. These new SP-containing AuNPs were characterized by a variety of analytical techniques, including TEM and DLS measurements and UV-vis and CD spectroscopy, which proved their high in vitro stability and poor tendency to interact with plasma proteins. Their labeling with diagnostic and therapeutic radionuclides was efficiently performed by DOTA complexation with the trivalent radiometals 67Ga and 177Lu or by electrophilic radioiodination with 125I of the tyrosyl residue in AuNP-SPTyr8. Cellular studies of the resulting radiolabeled AuNPs in NKR1-positive GBM cells (U87, T98G and U373) have shown that the presence of the SP peptides has a crucial and positive impact on their internalization by the tumor cells. Consistently, 177Lu-AuNP-SPTyr8 showed more pronounced radiobiological effects in U373 cells when compared with the non-targeted congener 177Lu-AuNP-TDOTA, as assessed by cell viability and clonogenic assays and corroborated by Monte Carlo microdosimetry simulations.

(Plasmonic gold core)@(ultrathin ruthenium shell) nanostructures as antenna-reactor photocatalysts toward nitrogen photofixation

Ruthenium (Ru) is known as the optimal metal catalyst for ammonia (NH3) synthesis, but the poor light-harvesting capability restricts its application in photocatalysis. Herein, we construct an antenna-reactor nanostructure through...

HA/PEI-coated acridine orange-loaded gold-core silica shell nanorods for cancer-targeted photothermal and chemotherapy

Aims: To develop a tumor-targeted chemo-photothermal nanomedicine through the functionalization of acridine orange (AO)-loaded gold-core mesoporous silica shell (AuMSS) nanorods with polyethylenimine (PEI) and hyaluronic acid (HA). Methods: Functionalization of the AuMSS nanorods was achieved through the chemical linkage of PEI followed by electrostatic adsorption of HA. Results: HA functionalization improved AuMSS' cytocompatibility by decreasing blood hemolysis, and PEI-HA inclusion promoted a controlled and sustained AO release. In vitro assays revealed that HA functionalization increased the internalization of nanoparticles by human negroid cervix epithelioid carcinoma cancer (HeLa) cells, and the combinatorial treatment mediated by AuMSS/PEI/HA_AO nanorods presented an enhanced effect, with >95% of cellular death. Conclusion: AuMSS/PEI/HA_AO formulations can act as tumor-targeted chemo-photothermal nanomedicines for the combinatorial therapy of cervical cancer.

Gold rush: the political economy of the Yugoslavian gold exchange standard

This article analyses the economic, political and cultural factors that influenced the decision of policy-makers in Yugoslavia to join the gold exchange standard in the midst of the Great Depression in June 1931. The analysis proceeds in three stages. First, the economic reasons why policy elites and interest groups endeavoured to adopt the gold exchange standard are examined by looking at debates in Yugoslavia's central bank, correspondence between governmental institutions and the views of policy elites as depicted in various economic newspapers. Subsequently, the article analyses how the beliefs in economic benefits analysed in the previous part were formed, considering the state of economic knowledge in the country, as well as pressures exerted by foreign lenders such as the Bank of England, the Banque de France and the Bank for International Settlements. The third part analyses reasons for legal stabilisation that go beyond economic rationales, considering how the government employed the prestige involved in legal stabilisation for its political agenda, and how cultural attachments to ‘gold core countries’ made sharing their monetary system a matter of cultural integration.