A potent small-molecule inducer of chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells

2012 ◽  
Vol 3 (10) ◽  
pp. 3071 ◽  
Author(s):  
Tae-Jun Cho ◽  
Jonghoon Kim ◽  
Soon-Keun Kwon ◽  
Keunhee Oh ◽  
Jeong-ae Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Small Molecule ◽  
Chondrogenic Differentiation ◽  
Human Bone ◽  
Human Bone Marrow

scholarly journals Osteoporosis is accompanied by reduced CD274 expression in human bone marrow-derived mesenchymal stem cells

2021 ◽  
Vol 41 ◽  
pp. 603-615
Author(s):  
A-N Zeller ◽  
◽  
M Selle ◽  
Z Gong ◽  
M Winkelmann ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Surface ◽  
Chondrogenic Differentiation ◽  
Surface Antigens ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Differentiation Potential ◽  
Proliferation Potential

Underlying pathomechanisms of osteoporosis are still not fully elucidated. Cell-based therapy approaches pose new possibilities to treat osteoporosis and its complications. The aim of this study was to quantify differences in human bone marrow-derived mesenchymal stem cells (hBMSCs) between healthy donors and those suffering from clinically manifest osteoporosis. Cell samples of seven donors for each group were selected retrospectively from the hBMSC cell bank of the Trauma Department of Hannover Medical School. Cells were evaluated for their adipogenic, osteogenic and chondrogenic differentiation potential, for their proliferation potential and expression of surface antigens. Furthermore, a RT2 Osteoporosis Profiler PCR array, as well as quantitative real-time PCR were carried out to evaluate changes in gene expression. Cultivated hBMSCs from osteoporotic donors showed significantly lower cell surface expression of CD274 (4.98 % ± 2.38 %) than those from the control group (26.03 % ± 13.39 %; p = 0.007), as assessed by flow cytometry. In osteoporotic patients, genes involved in inhibition of the anabolic WNT signalling pathway and those associated with stimulation of bone resorption were significantly upregulated. Apart from these changes, no significant differences were found for the other cell surface antigens, adipogenic, osteogenic and chondrogenic differentiation ability as well as proliferation potential. These findings supported the theory of an influence of CD274 on the regulation of bone metabolism. CD274 might be a promising target for further investigations of the pathogenesis of osteoporosis and of cell-based therapies involving MSCs.

Mesenchymal Stem Cells Derived from Human Bone Marrow and Adipose Tissue Maintain Their Immunosuppressive Properties After Chondrogenic Differentiation: Role of HLA-G

2016 ◽  
Vol 25 (19) ◽  
pp. 1454-1469 ◽  
Author(s):  
Wen-Jing Du ◽  
Loic Reppel ◽  
Léonore Leger ◽  
Chantal Schenowitz ◽  
Celine Huselstein ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Chondrogenic Differentiation ◽  
Human Bone ◽  
Human Bone Marrow

Dynamic Regulation of TWIST1 Expression During Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells

2017 ◽  
Vol 26 (10) ◽  
pp. 751-761 ◽  
Author(s):  
Mairéad A. Cleary ◽  
Roberto Narcisi ◽  
Anna Albiero ◽  
Florien Jenner ◽  
Laurie M.G. de Kroon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Chondrogenic Differentiation ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Dynamic Regulation ◽  
Twist1 Expression

Metabolic Labeling of Human Bone Marrow Mesenchymal Stem Cells for the Quantitative Analysis of their Chondrogenic Differentiation

2012 ◽  
Vol 11 (11) ◽  
pp. 5350-5361 ◽  
Author(s):  
Beatriz Rocha ◽  
Valentina Calamia ◽  
Jesús Mateos ◽  
Patricia Fernández-Puente ◽  
Francisco J. Blanco ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Quantitative Analysis ◽  
Chondrogenic Differentiation ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Metabolic Labeling ◽  
Marrow Mesenchymal Stem Cells

Enhanced chondrogenic differentiation of human bone marrow mesenchymal stem cells on PCL/PLGA electrospun with different alignments and compositions

2017 ◽  
Vol 67 (1) ◽  
pp. 50-60 ◽  
Author(s):  
Soheila Zamanlui ◽  
Matin Mahmoudifard ◽  
Masoud Soleimani ◽  
Behnaz Bakhshandeh ◽  
Mohammad Vasei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Chondrogenic Differentiation ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Marrow Mesenchymal Stem Cells
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