Amplified fluorescence sensing of miRNA by combination of graphene oxide with duplex-specific nuclease

2014 ◽  
Vol 6 (11) ◽  
pp. 3598-3603 ◽  
Author(s):  
Shuang Guo ◽  
Fan Yang ◽  
Yulin Zhang ◽  
Yong Ning ◽  
Qunfeng Yao ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Signal Amplification ◽  
Sensitive Detection ◽  
Fluorescence Sensing ◽  
Fluorescent Sensing ◽  
Sensing System ◽  
Amplification Strategy

A graphene oxide-based fluorescent sensing system for sensitive detection of miRNA with a duplex-specific nuclease-aided signal amplification strategy.

An enzyme-aided amplification strategy for sensitive detection of DNA utilizing graphene oxide (GO) as a fluorescence quencher

The Analyst ◽  
2014 ◽  
Vol 139 (13) ◽  
pp. 3455-3459 ◽  
Author(s):  
Jing Zhang ◽  
Mangjuan Tao ◽  
Yan Jin
Keyword(s):  
Graphene Oxide ◽  
Rapid Method ◽  
Signal Amplification ◽  
Sensitive Detection ◽  
Lambda Exonuclease ◽  
Amplification Strategy ◽  
Fluorescence Quencher

A facile, sensitive and rapid method has been developed for detection of disease-related DNA based on lambda exonuclease-aided signal amplification by utilizing graphene oxide (GO) as a fluorescence quencher.

scholarly journals Amplified Fluorescent Aptasensor for Ochratoxin A Assay Based on Graphene Oxide and RecJf Exonuclease

Toxins ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 670
Author(s):  
Han Zhao ◽  
Dehui Xiong ◽  
Ying Yan ◽  
Changbei Ma
Keyword(s):  
Graphene Oxide ◽  
Ochratoxin A ◽  
Signal Amplification ◽  
Red Wine ◽  
Limit Of Detection ◽  
Experimental Conditions ◽  
Fluorescent Sensing ◽  
Sensing System ◽  
Sensing Platform ◽  
Fluorescent Response

In this study, we developed an aptamer-based fluorescent sensing platform for the detection of ochratoxin A (OTA) based on RecJf exonuclease-assisted signal amplification and interaction between graphene oxide (GO) and the OTA aptamer (OTA-apt). After optimizing the experimental conditions, the present aptamer-based sensing system can exhibit excellent fluorescent response in the OTA assay, with a limit of detection of 0.07 ng/mL. In addition to signal amplification, this strategy is also highly specific for other interfering toxins. Furthermore, this aptasensor can be reliably used for assessing red wine samples spiked with different OTA concentrations (2.4, 6 and 20 ng/mL). The proposed assay plays an important role in the field of food safety and can be transformed for detecting other toxins by replacing the sequence that recognizes the aptamer.

scholarly journals A new two-mode fluorescence signal amplification strategy for protease activity assay based on graphene oxide

RSC Advances ◽  
2017 ◽  
Vol 7 (76) ◽  
pp. 47983-47989 ◽  
Author(s):  
Fangfang Wang ◽  
Jie Gao ◽  
Jianwei Zhao ◽  
Wenyue Zhang ◽  
Jie Bai ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Signal Amplification ◽  
Protease Activity ◽  
Fluorescence Signal ◽  
Activity Assay ◽  
Fluorescence Signal Amplification ◽  
Amplification Strategy

A new graphene oxide-based two-mode fluorescence signal amplification strategy for the detection of protease activity has been established.

Homogeneous electrochemical aptasensor based on a dual amplification strategy for sensitive detection of profenofos residues

2018 ◽  
Vol 42 (17) ◽  
pp. 14642-14647 ◽  
Author(s):  
Yancui Jiao ◽  
Jiayun Fu ◽  
Wenjie Hou ◽  
Zhaoqiang Shi ◽  
Yemin Guo ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Sensitive Detection ◽  
Homogeneous Type ◽  
Electrochemical Aptasensor ◽  
Dual Amplification ◽  
Amplification Strategy

A homogeneous type of electrochemical aptasensor was designed based upon the principle of target-induced and tool enzyme-assisted signal amplification, which was employed for the detection of profenofos residues.

scholarly journals A fluorescence sensing platform of theophylline based on the interaction of RNA aptamer with graphene oxide

RSC Advances ◽  
2019 ◽  
Vol 9 (34) ◽  
pp. 19813-19818 ◽  
Author(s):  
Jian-Feng Wu ◽  
Xin Gao ◽  
Ling Ge ◽  
Guang-Chao Zhao ◽  
Guang-Feng Wang
Keyword(s):  
Graphene Oxide ◽  
Rna Aptamer ◽  
Fluorescence Sensing ◽  
Fluorescent Sensing ◽  
Sensing Platform

A fluorescent sensing platform of theophylline based on the interaction of an RNA aptamer with GO and CdTe as the signal.

A hyperbranched transcription-activated CRISPR-Cas12a signal amplification strategy for sensitive microRNA sensing

2020 ◽  
Vol 56 (87) ◽  
pp. 13445-13448
Author(s):  
Weimin Tian ◽  
Xiaoling Liu ◽  
Gaoting Wang ◽  
Chenghui Liu
Keyword(s):  
Signal Amplification ◽  
Sensitive Detection ◽  
Amplification Strategy

A hyperbranched RCA-assisted transcription and CRISPR-Cas12a-based triplex signal amplification strategy is developed for the sensitive detection of microRNA.

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