Application of titanium dioxide nanowires and electroreduced graphene oxide modified electrodes for the electrochemical detection of specific tlh gene sequence from Vibrio parahaemolyticus

2015 ◽  
Vol 7 (6) ◽  
pp. 2623-2629 ◽  
Author(s):  
Xiuli Wang ◽  
Guangjiu Li ◽  
Lihua Liu ◽  
Yong Cheng ◽  
Wen Zheng ◽  
...  

Electrochemical DNA biosensor was prepared using electroreduced graphene oxide, titanium dioxide nanowires and chitosan modified carbon ionic liquid electrode for the detection of the specific tlh gene sequence.

2012 ◽  
Vol 178 (1-2) ◽  
pp. 211-219 ◽  
Author(s):  
Song Hu ◽  
Huanhuan Zhu ◽  
Shengyun Liu ◽  
Jun Xiang ◽  
Wei Sun ◽  
...  

2021 ◽  
Vol 17 ◽  
Author(s):  
Lijun Yan ◽  
Fan Shi ◽  
Jingyao Zhang ◽  
Yanyan Niu ◽  
Lifang Huang ◽  
...  

Background: By using bimetal nanocomposite modified electrode, the electrochemical DNA biosensor showed the advantages of high sensitivity, low cost, rapid response and convenient operation, which was applied for disease diagnosis, food safety, and biological monitoring. Objective: A nanocomposite consisting of platinum (Pt)-gold (Au) bimetal and two-dimensional graphene (GR) was synthesized by hydrothermal method, which was modified on the surface of carbon ionic liquid electrode and further used for the immobilization of probe ssDNA related to Vibrio Parahaemolyticus tlh gene to construct an electrochemical DNA sensor. Method: Potassium ferricyanide was selected as electrochemical indicator, cyclic voltammetry was used to study the electrochemical behaviours of different modified electrodes and differential pulse voltammetry was employed to test the analytical performance of this biosensor for the detection of target gene sequence. Results: This electrochemical DNA biosensor could detect the Vibrio Parahaemolyticus tlh gene sequence as the linear concentration in the range from 1.0×10-13 mol L-1 to 1.0×10-6 mol L-1 with the detection limit as 2.91×10-14 mol L-1 (3σ). Conclusion: This proposed electrochemical DNA biosensor could be used to identify the special gene sequence with good selectivity, low detection limit and wide detection range.


2010 ◽  
Vol 57 (6) ◽  
pp. 1367-1373 ◽  
Author(s):  
He-Jian Liu ◽  
Li-Ning Qu ◽  
Song Hu ◽  
Tian-Rong Zhan ◽  
Chao-Zhi Zhao ◽  
...  

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