Electrochemical DNA biosensor based on platinum-gold bimetal decorated graphene modified electrode for the detection of Vibrio Parahaemolyticus specific tlh gene sequence

2021 ◽  
Vol 17 ◽  
Author(s):  
Lijun Yan ◽  
Fan Shi ◽  
Jingyao Zhang ◽  
Yanyan Niu ◽  
Lifang Huang ◽  
...  
Keyword(s):  
Detection Limit ◽  
Modified Electrode ◽  
Vibrio Parahaemolyticus ◽  
Gene Sequence ◽  
Modified Electrodes ◽  
Disease Diagnosis ◽  
Dna Biosensor ◽  
Carbon Ionic Liquid Electrode ◽  
Detection Range ◽  
Electrochemical Dna Biosensor

Background: By using bimetal nanocomposite modified electrode, the electrochemical DNA biosensor showed the advantages of high sensitivity, low cost, rapid response and convenient operation, which was applied for disease diagnosis, food safety, and biological monitoring. Objective: A nanocomposite consisting of platinum (Pt)-gold (Au) bimetal and two-dimensional graphene (GR) was synthesized by hydrothermal method, which was modified on the surface of carbon ionic liquid electrode and further used for the immobilization of probe ssDNA related to Vibrio Parahaemolyticus tlh gene to construct an electrochemical DNA sensor. Method: Potassium ferricyanide was selected as electrochemical indicator, cyclic voltammetry was used to study the electrochemical behaviours of different modified electrodes and differential pulse voltammetry was employed to test the analytical performance of this biosensor for the detection of target gene sequence. Results: This electrochemical DNA biosensor could detect the Vibrio Parahaemolyticus tlh gene sequence as the linear concentration in the range from 1.0×10-13 mol L-1 to 1.0×10-6 mol L-1 with the detection limit as 2.91×10-14 mol L-1 (3σ). Conclusion: This proposed electrochemical DNA biosensor could be used to identify the special gene sequence with good selectivity, low detection limit and wide detection range.

Application of titanium dioxide nanowires and electroreduced graphene oxide modified electrodes for the electrochemical detection of specific tlh gene sequence from Vibrio parahaemolyticus

2015 ◽  
Vol 7 (6) ◽  
pp. 2623-2629 ◽  
Author(s):  
Xiuli Wang ◽  
Guangjiu Li ◽  
Lihua Liu ◽  
Yong Cheng ◽  
Wen Zheng ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Titanium Dioxide ◽  
Graphene Oxide ◽  
Electrochemical Detection ◽  
Vibrio Parahaemolyticus ◽  
Gene Sequence ◽  
Modified Electrodes ◽  
Carbon Ionic Liquid Electrode ◽  
Electrochemical Dna Biosensor ◽  
Liquid Electrode

Electrochemical DNA biosensor was prepared using electroreduced graphene oxide, titanium dioxide nanowires and chitosan modified carbon ionic liquid electrode for the detection of the specific tlh gene sequence.

Investigation of Electrochemical DNA Biosensor for Detection of Specific nuc Gene of Staphylococcus aureus Based on Gold Nanoparticles and Thiol Graphene Nanocomposite

2020 ◽  
Vol 16 ◽  
Author(s):  
Lijun Yan ◽  
Bo Shao ◽  
Xiaoping Zhang ◽  
Yanyan Niu ◽  
Wendi Dang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Gold Nanoparticles ◽  
Detection Limit ◽  
Electrochemical Impedance ◽  
Pcr Amplification ◽  
Dna Biosensor ◽  
Carbon Ionic Liquid Electrode ◽  
Electrochemical Performances ◽  
Detection Range ◽  
Electrochemical Dna Biosensor

Background: Based on gold nanoparticles (AuNPs) and thiol graphene (TGR) nanocomposite modified carbon ionic liquid electrode, an electrochemical DNA biosensor was prepared to detect specific nuc gene of Staphylococcus aureus, which was further used to the analysis of PCR amplification samples from unfrozen pork. Objective: The construction of DNA biosensor with AuNPs and TGR could be used as a new sensing platform to detect specific ssDNA sequence in real biological samples. Method: Electrochemical behaviors of working electrodes were studied by cyclic voltammetry and electrochemical impedance spectroscopy with electrochemical performances of this biosensor investigated by differential pulse voltammetry. Results: This gene sensor could detect the specific nuc gene of Staphylococcus aureus in the linear concentration range from 1.0×10-15 mol L-1 to 1.0×10-6 mol L-1 with the detection limit as 4.5×10-16 mol L-1 (3σ), and it was applied to the detection of PCR amplification sample of Staphylococcus aureus with satisfactory results. Conclusion: This gene biosensor showed high sensitivity and good selectivity, wide detection range and low detection limit, which demonstrated an effective tool to detect specific nuc gene sequences of Staphylococcus aureus.

A sensitive electrochemical DNA biosensor for Microcystis spp. sequence detection based on an Ag@Au NP composite film

2015 ◽  
Vol 7 (7) ◽  
pp. 2993-2999 ◽  
Author(s):  
Ping Tong ◽  
Yanhong Shao ◽  
Jinfeng Chen ◽  
Yu He ◽  
Lan Zhang
Keyword(s):  
Detection Limit ◽  
Composite Film ◽  
Gene Sequence ◽  
Dna Biosensor ◽  
Sequence Detection ◽  
Electrochemical Dna Biosensor

An electrochemical DNA biosensor is developed for detecting a gene sequence related to blooming genera of Cyanobacteria, Microcystis spp. based on an Ag@Au NP composite film. The detection limit was low to 1.6×10−12 mol L−1.

scholarly journals Electrochemical determination of ascorbic acid at p-phenylenediamine film-holes modified glassy carbon electrode

2015 ◽  
Vol 80 (9) ◽  
pp. 1161-1175 ◽  
Author(s):  
Bikila Olana ◽  
Shimeles Kitte ◽  
Tesfaye Soreta
Keyword(s):  
Ascorbic Acid ◽  
Detection Limit ◽  
Glassy Carbon Electrode ◽  
Modified Electrode ◽  
Glassy Carbon ◽  
Linear Response ◽  
Modified Electrodes ◽  
Electrochemical Determination ◽  
Carbon Electrode

In this work the determination of ascorbic acid (AA) at glassy carbon electrode (GCE) modified with a perforated film produced by reduction of diazonium generated in situ from p-phenylenediamine (PD) is reported. Holes were intentionally created in the modifier film by stripping a pre-deposited gold nanoparticles. The modified electrodes were electrochemically characterized by common redox probes: hydroquinone, ferrocyanide and hexamineruthenium(III). The cyclic voltammetric and amperometric response of AA using the modified electrodes was compared with that of bare GCE. The bare GCE showed a linear response to AA in the concentration range of 5 mM to 45 mM with detection limit of 1.656 mM and the modified GCE showed a linear response to AA in the concentration range of 5 ?M to 45 ?M with detection limit of 0.123 ?M. The effect of potential intereferents on amperometric signal of AA at the modified GCE was examined and found to be minimal. The inter-electrode reproducibility, stability, and accuracy were determined. The modified electrode showed excellent inter-electrode reproducibility, accuracy and stability. The modified electrode reported is a promising candidate for use in electroanalysis of AA.

Reduced graphene oxide nanoribbon immobilized gold nanoparticle based electrochemical DNA biosensor for the detection of Mycobacterium tuberculosis

2018 ◽  
Vol 6 (31) ◽  
pp. 5181-5187 ◽  
Author(s):  
Navin Kumar Mogha ◽  
Vikrant Sahu ◽  
Raj Kishore Sharma ◽  
Dhanraj T. Masram
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Graphene Oxide ◽  
Detection Limit ◽  
Gold Nanoparticle ◽  
Reduced Graphene Oxide ◽  
High Specificity ◽  
Dna Biosensor ◽  
Electrochemical Dna Biosensor ◽  
Reduced Graphene ◽  
Very High

A DNA based biosensor is reported with very high specificity to Mycobacterium tuberculosis and a detection limit much superior to those already reported in the literature.

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