A colorimetric immunoassay for respiratory syncytial virus detection based on gold nanoparticles–graphene oxide hybrids with mercury-enhanced peroxidase-like activity

2014 ◽  
Vol 50 (78) ◽  
pp. 11526-11528 ◽  
Author(s):  
Lei Zhan ◽  
Chun Mei Li ◽  
Wen Bi Wu ◽  
Cheng Zhi Huang
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Respiratory Syncytial Virus ◽  
Virus Detection ◽  
Colorimetric Immunoassay ◽  
Syncytial Virus

A novel colorimetric immunoassay for detection of respiratory syncytial virus (RSV) was developed based on mercury-stimulated peroxidase-like activity of gold nanoparticles–graphene composites (AuNPs–GO).

scholarly journals Electrochemical Immunosensors Based on Screen-Printed Gold and Glassy Carbon Electrodes: Comparison of Performance for Respiratory Syncytial Virus Detection

Biosensors ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 175
Author(s):  
Wioleta Białobrzeska ◽  
Daniel Firganek ◽  
Maciej Czerkies ◽  
Tomasz Lipniacki ◽  
Marta Skwarecka ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Glassy Carbon ◽  
Dynamic Range ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Electrochemical Characterizations ◽  
Virus Detection ◽  
Electrochemical Immunosensors ◽  
Syncytial Virus ◽  
Screen Printed

This paper presents the development and comparison of label-free electrochemical immunosensors based on screen-printed gold and glassy carbon (GC) disc electrodes for efficient and rapid detection of respiratory syncytial virus (RSV). Briefly, the antibody specific to the F protein of RSV was successfully immobilized on modified electrodes. Antibody coupling on the Au surface was conducted via 4-aminothiophenol (4-ATP) and glutaraldehyde (GA). The GC surface was modified with poly-L-lysine (PLL) for direct anti-RSV conjugation after EDC/NHS (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-Hydroxysuccinimide) activation. Electrochemical characterizations of the immunosensors were carried out by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). GC-based immunosensors show a dynamic range of antigen detection from 1.0 × 105 PFU/mL to 1.5×107 PFU/mL, more than 1.0 × 105 PFU/mL to 1.0 × 107 PFU/mL for the Au-based sensor. However, the GC platform is less sensitive and shows a higher detection limit (LOD) for RSV. The limit of detection of the Au immunosensor is 1.1 × 103 PFU/mL, three orders of magnitude lower than 2.85 × 106 PFU/mL for GC. Thus, the Au-based immunosensor has better analytical performance for virus detection than a carbon-based platform due to high sensitivity and very low RSV detection, obtained with good reproducibility.

scholarly journals Real-Time Light Scattering Tracking of Gold Nanoparticles- bioconjugated Respiratory Syncytial Virus Infecting HEp-2 Cells

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Xiao-Yan Wan ◽  
Lin-Ling Zheng ◽  
Peng-Fei Gao ◽  
Xiao-Xi Yang ◽  
Chun-Mei Li ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Respiratory Syncytial Virus ◽  
Light Scattering ◽  
Real Time ◽  
Syncytial Virus

Evaluation of Respiratory Syncytial Virus Detection by Rapid Antigen Tests in Childhood

2007 ◽  
Vol 219 (4) ◽  
pp. 212-216 ◽  
Author(s):  
U. Schauer ◽  
G. Ihorst ◽  
A. Rohwedder ◽  
G. Petersen ◽  
R. Berner ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Virus Detection ◽  
Syncytial Virus ◽  
Antigen Tests

Respiratory and Enteric Virus Detection in Children

2016 ◽  
Vol 32 (1) ◽  
pp. 84-93 ◽  
Author(s):  
Marko Pokorn ◽  
Monika Jevšnik ◽  
Miroslav Petrovec ◽  
Andrej Steyer ◽  
Tatjana Mrvič ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Febrile Seizure ◽  
Viral Infections ◽  
Virus Detection ◽  
Enteric Virus ◽  
Febrile Seizures ◽  
Healthy Controls ◽  
Human Coronavirus ◽  
Detection Rates ◽  
Syncytial Virus

The majority of children with febrile seizures have viral infections and viruses were detected in 22% to 63% of children in published studies. Using molecular methods, viruses were also detected in asymptomatic persons. A prospective study was conducted to detect respiratory and enteric viruses in 192 children with febrile seizures and compare the detection rates to those found in 156 healthy age-matched controls. A respiratory or enteric virus was detected in 72.9% of children with febrile seizures and in 51.4% of healthy controls. The viruses most strongly associated with febrile seizures were influenza, respiratory syncytial virus, parainfluenza, human coronavirus, and rotavirus. Compared to healthy controls, the age-adjusted odds ratios for nasopharynx virus positivity in febrile seizure patients were 79.4, 2.8, 7.2, and 4.9 for influenza virus, parainfluenza virus, respiratory syncytial virus, and human coronavirus, respectively, and 22.0 for rotavirus in stool. The detected virus did not influence clinical features of febrile seizure.

Survey of WU and KI polyomaviruses, coronaviruses, respiratory syncytial virus and parechovirus in children under 5 years of age in Tehran, Iran

2020 ◽  
Author(s):  
Fahimeh Sadat Aghamirmohammadali ◽  
Kaveh Sadeghi ◽  
Nazanin Zahra Shafiei-Jandaghi ◽  
Zahra Khoban ◽  
Talat Mokhtari-Azad ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Respiratory Infections ◽  
Virus Detection ◽  
Respiratory Viruses ◽  
Hospitalized Children ◽  
Acute Respiratory Infections ◽  
Older Children ◽  
Viral Pathogen ◽  
Syncytial Virus ◽  
Tract Infections

Background and Objectives: Severe acute respiratory infections (SARI) remain an important cause for childhood morbid- ity worldwide. We designed a research with the objective of finding the frequency of respiratory viruses, particularly WU and KI polyomaviruses (WUPyV & KIPyV), human coronaviruses (HCoVs), human respiratory syncytial virus (HRSV) and human parechovirus (HPeV) in hospitalized children who were influenza negative. Materials and Methods: Throat swabs were collected from children younger than 5 years who have been hospitalized for SARI and screened for WUPyV, KIPyV, HCoVs, HRSV and HPeV using Real time PCR. Results: A viral pathogen was identified in 23 (11.16%) of 206 hospitalized children with SARI. The rate of virus detection was considerably greater in infants <12 months (78.2%) than in older children (21.8%). The most frequently detected vi- ruses were HCoVs with 7.76% of positive cases followed by KIPyV (2%) and WUPyV (1.5%). No HPeV and HRSV were detected in this study. Conclusion: This research shown respiratory viruses as causes of childhood acute respiratory infections, while as most of mentioned viruses usually causes mild respiratory diseases, their frequency might be higher in outpatient children. Mean- while as HRSV is really sensitive to inactivation due to environmental situations and its genome maybe degraded, then for future studies, we need to use fresh samples for HRSV detection. These findings addressed a need for more studies on viral respiratory tract infections to help public health.

scholarly journals Human respiratory syncytial virus detection in children admitted at a community hospital in Botucatu, SP, Brazil

2004 ◽  
Vol 35 (4) ◽  
pp. 348-351 ◽  
Author(s):  
Patrícia Alves Ramos Bosso ◽  
João Manuel Grisi Candeias ◽  
Karina dos Santos Paduan ◽  
Sandra Mara Queiroz Ricchetti ◽  
Antero Frederico Macedo de Miranda ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Community Hospital ◽  
Virus Detection ◽  
Human Respiratory Syncytial Virus ◽  
Syncytial Virus

scholarly journals Respiratory syncytial virus (RSV): a scourge from infancy to old age

Thorax ◽  
2019 ◽  
Vol 74 (10) ◽  
pp. 986-993 ◽  
Author(s):  
James Andrew Coultas ◽  
Rosalind Smyth ◽  
Peter J Openshaw
Keyword(s):  
Respiratory Syncytial Virus ◽  
Virus Detection ◽  
Respiratory Illness ◽  
The Elderly ◽  
Surface Glycoprotein ◽  
Detection Methods ◽  
School Age Children ◽  
Elderly Persons ◽  
Populations At Risk ◽  
Syncytial Virus

Respiratory syncytial virus (RSV) is the most common single cause of respiratory hospitalisation of infants and is the second largest cause of lower respiratory infection mortality worldwide. In adults, RSV is an under-recognised cause of deterioration in health, particularly in frail elderly persons. Infection rates typically rise in late autumn and early winter causing bronchiolitis in infants, common colds in adults and insidious respiratory illness in the elderly. Virus detection methods optimised for use in children have low detection rate in adults, highlighting the need for better diagnostic tests. There are many vaccines under development, mostly based on the surface glycoprotein F which exists in two conformations (prefusion and postfusion). Much of the neutralising antibody appears to be to the prefusion form. Vaccines being developed include live attenuated, subunit, particle based and live vectored agents. Different vaccine strategies may be appropriate for different target populations: at-risk infants, school-age children, adult caregivers and the elderly. Antiviral drugs are in clinical trial and may find a place in disease management. RSV disease is one of the major remaining common tractable challenges in infectious diseases and the era of vaccines and antivirals for RSV is on the near horizon.

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