A label-free conjugated polymer-based fluorescence assay for the determination of adenosine triphosphate and alkaline phosphatase

Abstract The manual procedure of Babson et al. (1) for the determination of serum alkaline phosphatase has been automated. The assay is based on the colorimetric determination of phenolphthalein formed on hydrolysis of phenolphthalein monophosphate. The procedure utilizes 0.16 ml. of serum without dialysis. Blanks are required only for turbid sera. Results are compared with those obtained by the manual procedure, and data relating to sample interaction, precision, blank values, and normal values are presented.

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Determination of the scissile bond in the hydrolysis of α-d-ribofuranose 1-phosphate by alkaline phosphatase, acid phosphatase and formic acid and in its conversion to d-ribose 5-phosphate by phosphoglucomutase

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology ◽

10.1016/0167-4838(82)90064-4 ◽

1982 ◽

Vol 704 (3) ◽

pp. 427-436 ◽

Cited By ~ 1

Author(s):

Frank Jordan ◽

Donald J. Kuo ◽

Salvatore J. Salamone ◽

Alice L. Wang

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Formic Acid ◽

Scissile Bond ◽

Hydrolysis Of

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A turn-on fluorescence assay of alkaline phosphatase activity using a DNA–silver nanocluster probe

New Journal of Chemistry ◽

10.1039/c7nj04894g ◽

2018 ◽

Vol 42 (6) ◽

pp. 4331-4336 ◽

Cited By ~ 9

Author(s):

Changbei Ma ◽

Haisheng Liu ◽

Kefeng Wu ◽

Mingjian Chen ◽

Hailun He ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Phosphatase Activity ◽

Alkaline Phosphatase Activity ◽

Fluorescence Assay ◽

Label Free ◽

Silver Nanocluster ◽

Turn On

A label-free fluorescence assay has been developed for the detection of alkaline phosphatase based on DNA–silver nanocluster probes.

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Cerium(iii)-directed assembly of glutathione-capped gold nanoclusters for sensing and imaging of alkaline phosphatase-mediated hydrolysis of adenosine triphosphate

Nanoscale ◽

10.1039/c8nr05050c ◽

2018 ◽

Vol 10 (37) ◽

pp. 17691-17698 ◽

Cited By ~ 17

Author(s):

Jyun-Guo You ◽

Chi-Yu Lu ◽

A. Santhana Krishna Kumar ◽

Wei-Lung Tseng

Keyword(s):

Alkaline Phosphatase ◽

Adenosine Triphosphate ◽

Gold Nanoclusters ◽

Directed Assembly ◽

Gold Clusters ◽

Enzymatic Reactions ◽

Aggregation Induced Emission ◽

Emission Enhancement ◽

Hydrolysis Of

The aggregation-induced emission enhancement of glutathione-capped gold clusters triggered by cerium(iii) is well-suited for the detection of adenosine triphosphate-related enzymatic reactions.

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Determination of L-Argininamide Based on Water-Soluble Fluorescent Conjugated Polymer-Aptamer

Journal of Analytical Methods in Chemistry ◽

10.1155/2013/682134 ◽

2013 ◽

Vol 2013 ◽

pp. 1-5 ◽

Cited By ~ 1

Author(s):

Hongliang Guan ◽

Zhike He

Keyword(s):

Conjugated Polymer ◽

Specific Binding ◽

Water Soluble ◽

Dna Sensor ◽

Label Free ◽

Binding Reaction ◽

Molecular Sensors ◽

Free Dna ◽

Highly Sensitive

Water-soluble fluorescent conjugated polymer is a promising material which could be used as an optical platform in highly sensitive molecular sensors. In this paper, a simple label-free DNA sensor, which consisted of a poly(3-alkoxy-4-methylthiophene) and an aptamer, was used to detect L-argininamide (L-Arm). Due to the specific binding reaction between L-Arm and its aptamer, the proposed method can easily determinate the L-Arm through the recovery of fluorescence without any modification. Other ions or similar molecules had little effect on the detection. Moreover, there was a linear relationship between fluorescence intensity and the concentration of L-Arm. The detection limit of L-Arm was as low as 4.7 nM.

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