Effect of riboflavin concentration on the development of photo-cross-linked amniotic membranes for cultivation of limbal epithelial cells

RSC Advances ◽  
2015 ◽  
Vol 5 (5) ◽  
pp. 3425-3434 ◽  
Author(s):  
Jui-Yang Lai ◽  
Li-Jyuan Luo
Keyword(s):  
Stem Cells ◽  
Epithelial Cells ◽  
Amniotic Membrane ◽  
Cross Linking ◽  
Collagen Network ◽  
Limbal Stem Cells ◽  
The Cross ◽  
Amniotic Membranes

Riboflavin concentration is critical to tailor the cross-linking degree of the collagen network and thus the nanostructure of photo-cross-linked amniotic membrane for cultivation of limbal stem cells.

Cornea and Lens Problems in Aniridia

2010 ◽  
Author(s):  
Edward J. Holland ◽  
Mayank Gupta
Keyword(s):  
Stem Cells ◽  
Epithelial Cells ◽  
Corneal Epithelium ◽  
Basal Layer ◽  
Limbal Stem Cells ◽  
Corneal Epithelial Cells ◽  
Film Stability ◽  
Corneal Epithelial ◽  
Corneal Erosion ◽  
Peripheral Cornea

The corneal epithelium is a rapidly regenerating, stratified squamous epithelium. Homeostasis of corneal epithelial cells is an important prerequisite, not only for the integrity of the ocular surface, but also for the visual function. The maintenance of a healthy corneal epithelium under both normal and wound-healing conditions is achieved by a population of stem cells located in the basal layer of limbal epithelium. The Limbus represents the transition zone between the peripheral cornea and the bulbar conjunctiva. The stem cells from the limbus generate the transient amplifying cells that migrate, proliferate, and differentiate to replace lost or damaged corneal epithelial cells. In patients with aniridia, there is a primary dysfunction of these limbal stem cells (see Figure 6.1). The cornea is affected clinically in 90 percent of the patients with aniridia. In most cases, the cornea in aniridic patients appears normal and transparent during infancy and childhood. However, during the early teens, the cornea begins to show changes. The early changes are marked by the in-growth of opaque epithelium from the limbal region into the peripheral cornea, which represents conjunctival epithelial cells, goblet cells, and blood vessels in the corneal epithelium. These changes gradually progress toward the central cornea and may cause corneal epithelial erosions and epithelial abnormalities that eventually culminate in opacification of the corneal stroma, which leads to vision loss. With the gradual loss of limbal stem cells, the entire cornea becomes covered with conjunctival cells. Eventually, many patients develop total limbal stem cell deficiency. These abnormalities usually become more pronounced with aging. The corneal abnormalities seen in aniridia are collectively termed “aniridic keratopathy”. Significant corneal opacification may occasionally be the initial manifestation of aniridia. Abnormal tear film stability and meibomian gland dysfunction are also observed in patients with aniridia. This can lead to dry eyes, aggravating corneal erosion and ulceration observed in aniridic patients. Sometimes, aniridia is associated with “Peter’s anomaly,” in which central corneal opacity is present at birth along with defects in the corneal endothelium and Descemet’s membrane.

386 HORSE AMNION: A SOURCE OF MESENCHYMAL (AMSC) AND EPITHELIAL STEM CELLS

2010 ◽  
Vol 22 (1) ◽  
pp. 349 ◽  
Author(s):  
A. Lange Consiglio ◽  
B. Corradetti ◽  
D. Bizzaro ◽  
M. Cassano ◽  
F. Cremonesi
Keyword(s):  
Stem Cells ◽  
Epithelial Cells ◽  
Amniotic Membrane ◽  
Embryonic Stem ◽  
Specific Pattern ◽  
Limiting Factor ◽  
Epithelial Stem Cells ◽  
Culture Dish ◽  
Isolated Cells ◽  
Differentiation Potential

According to the developmental stage from which they are obtained, stem cells are classified as being embryonic, fetal, or adult. Embryonic stem cells have unlimited self-renewing capacity and multilineage differentiation potential, but the separation of these cells requires destruction of the embryo. Moreover, their clinical application seems to be hindered by the high tumorigenic rate after transplantation. Stem cells derived from adult tissues are considered to be more limited in their potential; although, they are currently the more versatile cells in the clinical field. However, the risk of the immunological rejection of the transplanted stem cells by the recipient is an important limiting factor. In human medicine, stem cells isolated from term placenta are the ideal candidates for disease treatment, specifically because of their plasticity and reduced immunogenicity. The aim of this work was to provide, for the first time, an isolation protocol and the characteristics of the stem cells from horse amniotic membrane, which hold potential uses in equine clinical regenerative medicine. Minimal criteria for stemness definition are adherence to plastic culture dish, formation of fibroblast colony forming units (CFU-F), specific pattern of surface antigen expression, and differentiation potential toward one or more lineages. The amnion is a thin, avascular membrane composed of an epithelial layer and an outer layer of connective tissue. From 3 samples of allantoamnion retrieved at delivery, each amniotic membrane was stripped from the overlying allantois and, for isolation of the epithelial cells, digested with trypsin. After removal of epithelial cells, the AMSC population was obtained by digestion with collagenase and DNase. The cellular yield from term amnion was 10-fold more epithelial cells than AMSC. Isolated cells readily attached to plastic culture dishes. Culture was established in DMEM-HG medium, supplemented with 10% serum and EGF, where the cells proliferated robustly. Epithelial cells displayed typical cuboidal morphology, whereas AMSC were fibroblast-like. Normally, 5 to 6 passages were achieved before proliferation decreased, with a mean of 13.08 and 26.5 cell population doublings after 31 days, respectively, for epithelial cells and AMSC. The mean frequency of CFU-F was, respectively, 1 : 283 and 1:111 for epithelial cells and AMSC. The 2 cellular lines expressed MSC mRNA markers (CD29, CD105, CD44) and were negative for CD34, which was expressed at the fifth passage in both cellular types. Osteogenic differentiation of epithelial stem cells and AMSC was confirmed by von Kossa stain and by an increased expression of osteocalcin and osteopontin. Our preliminary data showed that equine amnion holds apparent potential as a source of presumptive stem cells, which might have widespread clinical applications, but aspects including immunohistochemical study, preclinical experimentation, and immunological properties must be studied.

Embryo co-culture with bovine amniotic membrane stem cells can enhance the cryo-survival of IVF-derived bovine blastocysts comparable with co-culture with bovine oviduct epithelial cells

Zygote ◽  
2020 ◽  
pp. 1-6
Author(s):  
Shayan Nejat-Dehkordi ◽  
Ebrahim Ahmadi ◽  
Abolfazl Shirazi ◽  
Hassan Nazari ◽  
Naser Shams-Esfandabadi
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Epithelial Cells ◽  
Embryonic Development ◽  
Amniotic Membrane ◽  
Biological Activities ◽  
Survival Rates ◽  
Blastocyst Stage ◽  
Oviduct Epithelial Cells

Summary Culture conditions have a profound effect on the quality of in vitro-produced embryos. Co-culturing embryos with somatic cells has some beneficial effects on embryonic development. Considering the ability of stem cells to secrete a broad range of growth factors with different biological activities, we hypothesized that bovine amniotic membrane stem cells (bAMSCs) might be superior to bovine oviduct epithelial cells (BOECs) in supporting embryonic development and enhancing their cryo-survival. Bovine abattoir-derived oocytes were matured and fertilized in vitro. The resultant presumptive zygotes were then cultured up to the blastocyst stage in the following groups: (i) co-culture with bAMSCs, (ii) co-culture with BOECs, and (iii) cell-free culture (Con). Embryos that reached the blastocyst stage were vitrified and warmed, and their post-warming re-expansion, survival and hatching rates were evaluated after 72 h culture. Results showed that the cleavage, blastocyst, and 2 h post-warming re-expansion rates of embryos did not differ between groups. However, their survival rates in BOEC and bAMSC groups were significantly higher compared with the control (72.7, 75.6 and 37.5%, respectively, P < 0.05). In conclusion, our results showed that the cryo-survivability of IVF-derived bovine embryos could be improved through co-culturing with bAMSCs. Moreover, considering the possibility to provide multiple passages from bAMSCs compared with BOECs, due to their stemness properties and their ability to produce growth factors, the use of bAMSCs is a good alternative to BOECs in embryo co-culture systems.

Stem Cell-Like Characteristics of Corneal Epithelial Cells

2014 ◽  
Vol 998-999 ◽  
pp. 312-315
Author(s):  
Fan Wang ◽  
Bo Ren ◽  
Yi Ning Yan
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Epithelial Cells ◽  
Corneal Epithelium ◽  
Cell Activity ◽  
Stem Cell Marker ◽  
Corneal Tissue ◽  
Limbal Stem Cells ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial

Purpose: The adult corneal epithelium is maintained by a population of limbal stem cells (LSCs), transmembrane protein prominin, regarded as stem cell marker was investigated on mouse corneal tissue, to study weather contains CD133-expressing cells and their distribution. Methods: Enucleated mouse eyes were embedded in OCT and cryosections were performed for mmunohistochemical studies using the avidin-biotin-peroxidase complex (ABC) procedure. Meanwhile, dissected mouse corneas were analyzed by westernblot. Results: In the adult mouse, 13A4 immunoreactivity was detected at the apical side of superficial corneal epithelium, including the limbus region, but not by stroma and endothelium. 115 KDa protein was approved in corneal tissue by Westernblot. Conclusions: The stem cell activity does not occur along the limbus but presumably presented by small portion of corneal epithelial cells which may hold a similar properties of stem cells.

Culture of limbal stem cells on human amniotic membrane

2012 ◽  
Vol 13 (3) ◽  
pp. 513-519 ◽  
Author(s):  
Mª Esther Rendal-Vázquez ◽  
Anahí San-Luis-Verdes ◽  
Mª Teresa Yebra-Pimentel-Vilar ◽  
Isabel López-Rodríguez ◽  
Nieves Domenech-García ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Limbal Stem Cells

Cryopreservation of Human Limbal Stem Cells Ex Vivo Expanded on Amniotic Membrane

Cornea ◽  
2008 ◽  
Vol 27 (3) ◽  
pp. 327-333 ◽  
Author(s):  
Hui-Jung Yeh ◽  
Chao-Ling Yao ◽  
Hsin-I Chen ◽  
Huey-Chuan Cheng ◽  
Shiaw-Min Hwang
Keyword(s):  
Stem Cells ◽  
Amniotic Membrane ◽  
Ex Vivo ◽  
Limbal Stem Cells

Short-Term Results Analysis in the Allogenic Transplantation of Limbal Stem Cells Expanded on Amniotic Membrane in Patients with Bilateral Limbal Stem Cell Deficiency

2020 ◽  
Vol 36 (4) ◽  
pp. 238-246
Author(s):  
Juan Carlos Serna-Ojeda ◽  
Mariana García-Mejía ◽  
Enrique O. Graue-Hernández ◽  
Alejandro Navas ◽  
Yonathan Garfias
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Amniotic Membrane ◽  
Limbal Stem Cell Deficiency ◽  
Short Term ◽  
Limbal Stem Cells ◽  
Allogenic Transplantation ◽  
Limbal Stem Cell

scholarly journals Ocular surface reconstruction with suspended limbal stem cells delivered by sutureless fixated amniotic membrane patch

Cell Research ◽  
2008 ◽  
Vol 18 (S1) ◽  
pp. S79-S79
Author(s):  
Bingqian Liu ◽  
Jian Ge ◽  
Zhichong Wang ◽  
Xuerong Sun ◽  
Ruzhang Jiang
Keyword(s):  
Stem Cells ◽  
Surface Reconstruction ◽  
Amniotic Membrane ◽  
Ocular Surface ◽  
Limbal Stem Cells ◽  
Ocular Surface Reconstruction ◽  
Membrane Patch
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