scholarly journals Two-photon sensitive protecting groups operating via intramolecular electron transfer: uncaging of GABA and tryptophan

2015 ◽  
Vol 6 (4) ◽  
pp. 2419-2426 ◽  
Author(s):  
Karolina A. Korzycka ◽  
Philip M. Bennett ◽  
Eduardo Jose Cueto-Diaz ◽  
Geoffrey Wicks ◽  
Mikhail Drobizhev ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Photoinduced Electron Transfer ◽  
High Sensitivity ◽  
Protecting Groups ◽  
Intramolecular Electron Transfer ◽  
Modular Approach ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

We present a modular approach to photo-labile protecting groups based on photoinduced electron transfer, providing high sensitivity to two-photon excitation.

Plasmon induced electron transfer at gold–TiO2 interface under femtosecond near-IR two-photon excitation

2009 ◽  
Vol 518 (2) ◽  
pp. 861-864 ◽  
Author(s):  
Luchao Du ◽  
Akihiro Furube ◽  
Kohjiro Hara ◽  
Ryuzi Katoh ◽  
M. Tachiya
Keyword(s):  
Electron Transfer ◽  
Two Photon ◽  
Near Ir ◽  
Photon Excitation ◽  
Two Photon Excitation

Two-Photon Excitation of Gold Nanorods Interrupted by Extremely Fast Solvent-to-Metal Electron Transfer

2017 ◽  
Vol 121 (51) ◽  
pp. 28546-28555 ◽  
Author(s):  
Hai Zhu ◽  
Monalisa Garai ◽  
Zhihui Chen ◽  
Qing-Hua Xu
Keyword(s):  
Electron Transfer ◽  
Gold Nanorods ◽  
Two Photon ◽  
Photon Excitation ◽  
Metal Electron ◽  
Two Photon Excitation

Photoactivatable AMPA for the study of glutamatergic neuronal transmission using two-photon excitation

2021 ◽  
Author(s):  
Davide Deodato ◽  
Naeem Asad ◽  
Timothy M. Dore
Keyword(s):  
Glutamate Receptor ◽  
Temporal Resolution ◽  
Receptor Agonist ◽  
High Sensitivity ◽  
Glutamatergic Transmission ◽  
Two Photon ◽  
Photon Excitation ◽  
Neuronal Transmission ◽  
Glutamate Receptor Agonist ◽  
Two Photon Excitation

TMP-CyHQ-AMPA is a photoactivatable form of a glutamate receptor agonist that has high sensitivity to 2-photon excitation. It can be used to study glutamatergic transmission with exceptional spatial-temporal resolution in complex tissue preparations.

o-Nitrobenzyl Photolabile Protecting Groups with Red-Shifted Absorption: Syntheses and Uncaging Cross-Sections for One- and Two-Photon Excitation

2006 ◽  
Vol 12 (26) ◽  
pp. 6865-6879 ◽  
Author(s):  
Isabelle Aujard ◽  
Chouaha Benbrahim ◽  
Marine Gouget ◽  
Odile Ruel ◽  
Jean-Bernard Baudin ◽  
...  
Keyword(s):  
Cross Sections ◽  
Protecting Groups ◽  
Two Photon ◽  
Photon Excitation ◽  
Photolabile Protecting Groups ◽  
Two Photon Excitation

Scanning algorithms in high-sensitivity two-photon excitation microscopy for single-molecule investigations

2004 ◽  
Author(s):  
Giuseppe Chirico ◽  
Gabriele Malengo ◽  
Roberto Milani ◽  
Fabio Cannone ◽  
Silke Krol ◽  
...  
Keyword(s):  
Single Molecule ◽  
High Sensitivity ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Two-photon excitation microscopy in cellular biophysics

1995 ◽  
Vol 53 ◽  
pp. 62-63
Author(s):  
David W. Piston ◽  
Brian D. Bennett ◽  
Robert G. Summers
Keyword(s):  
Confocal Microscopy ◽  
Laser Beam ◽  
Duty Cycle ◽  
Excited Electronic State ◽  
Input Power ◽  
Two Photon ◽  
Simultaneous Absorption ◽  
Photon Excitation ◽  
Very High ◽  
Two Photon Excitation

Two-photon excitation microscopy (TPEM) provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging and photochemistry. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10-5 maintains the average input power on the order of 10 mW, only slightly greater than the power normally used in confocal microscopy.

Two-photon excitation fluorescence microscopy in living systems

1993 ◽  
Vol 51 ◽  
pp. 154-155 ◽  
Author(s):  
David W. Piston
Keyword(s):  
Confocal Microscopy ◽  
Fluorescence Microscopy ◽  
Singlet State ◽  
Excited Electronic State ◽  
Input Power ◽  
Two Photon ◽  
Simultaneous Absorption ◽  
Photon Excitation ◽  
Very High ◽  
Two Photon Excitation

Two-photon excitation fluorescence microscopy provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In our fluorescence experiments, the final excited state is the same singlet state that is populated during a conventional fluorescence experiment. Thus, the fluorophore exhibits the same emission properties (e.g. wavelength shifts, environmental sensitivity) used in typical biological microscopy studies. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10−5 maintains the average input power on the order of 10 mW, only slightly greater than the power normally used in confocal microscopy.

Computationally Assisted Design of High Signal-to-Noise Photoinduced Electron Transfer-Based Voltage-Sensitive Dyes

2020 ◽  
Author(s):  
Rishikesh Kulkarni ◽  
Anneliese Gest ◽  
Chun Kei Lam ◽  
Benjamin Raliski ◽  
Feroz James ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Dft Calculations ◽  
Photoinduced Electron Transfer ◽  
Density Functional ◽  
Embryonic Stem ◽  
High Sensitivity ◽  
Md Simulations ◽  
High Signal ◽  
Signal To Noise ◽  
Green Fluorescent

<p>High signal-to-noise optical voltage indicators will enable simultaneous interrogation of membrane potential in large ensembles of neurons. However, design principles for voltage sensors with high sensitivity and brightness remain elusive, limiting the applicability of voltage imaging. In this paper, we use molecular dynamics (MD) simulations and density functional theory (DFT) calculations to guide the design of a bright and sensitive green-fluorescent voltage-sensitive fluorophore, or VoltageFluor (VF dye), that uses photoinduced electron transfer (PeT) as a voltage-sensing mechanism. MD simulations predict an 11% increase in sensitivity due to membrane orientation, while DFT calculations predict an increase in fluorescence quantum yield, but a decrease in sensitivity due to a decrease in rate of PeT. We confirm these predictions by synthesizing a new VF dye and demonstrating that it displays the expected improvements by doubling the brightness and retaining similar sensitivity to prior VF dyes. Combining theoretical predictions and experimental validation has resulted in the synthesis of the highest signal-to-noise green VF dye to date. We use this new voltage indicator to monitor the electrophysiological maturation of human embryonic stem cell-derived medium spiny neurons. </p>

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