Novel artificial antigen synthesis for antibody production and development of an indirect competitive ELISA of cyanocobalamin

2015 ◽  
Vol 7 (12) ◽  
pp. 5275-5281 ◽  
Author(s):  
Fanfan Yang ◽  
Lixin Zhu ◽  
Wei Meng ◽  
Renrong Liu
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Antibody Production ◽  
Bovine Serum ◽  
Competitive Elisa ◽  
Current Work ◽  
Indirect Competitive Elisa ◽  
Artificial Antigen

In the current work, cyanocobalamin is coupled to Bovine Serum Albumin (BSA) and ovalbumin (OVA) by a CDI method to produce artificial antigens.

The production of polyclonal antibodies against the mycotoxin derivative patulin hemiglutarate

1993 ◽  
Vol 39 (9) ◽  
pp. 861-863 ◽  
Author(s):  
L. J. McElroy ◽  
C. M. Weiss
Keyword(s):  
Molecular Weight ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Antibody Titer ◽  
Bovine Serum ◽  
Polyclonal Antibodies ◽  
Competitive Elisa ◽  
Low Molecular Weight ◽  
Specific Antibodies ◽  
Indirect Competitive Elisa

The mycotoxin patulin is a toxic, carcinogenic, unsaturated lactone produced by a number of molds. Polyclonal antibodies against patulin hemiglutarate were produced. Specific antibodies against patulin alone, however, were not clearly demonstrated. Because of its low molecular weight, patulin required conjugation to bovine serum albumin (BSA) to increase its immunogenicity. Anti-patulin-hemiglutarate-BSA antibody titer and specificity were determined using indirect and indirect competitive ELISA, respectively. Immunoassays would facilitate detection and quantitation of patulin.Key words: patulin, mycotoxin, antibodies.

Antibody to an artificial disaccharide antigen cross-reactive with Neisseria gonorrhoeae lipopolysaccharide

1979 ◽  
Vol 57 (5) ◽  
pp. 367-371 ◽  
Author(s):  
David R. Bundle
Keyword(s):  
Cell Wall ◽  
Bovine Serum Albumin ◽  
Neisseria Gonorrhoeae ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Covalent Attachment ◽  
Passive Hemagglutination ◽  
Streptococcus Faecalis ◽  
Artificial Antigen

An artificial antigen was prepared from 4-O-β-D-galactopyranosyl-D-glucose (lactose) and 8-ethoxycarbonyloctanol. Covalent attachment to bovine serum albumin provided an antigen that elicited antilactose antibody in rabbits and goat. These antibodies were active against Neisseria gonorrhoeae lipopolysaccharide in passive hemagglutination tests. The same antibody agglutinated cells of Streptococcus faecalis, strain N, and precipitated the lactose-containing cell wall diheteroglycan of this organism. Fractionation of rabbit and goat antibody raised against the synthetic antigen of S. faecalis vaccine provided two antibody fractions only one of which, eluted from the immunoadsorbent by galactose, was active against N. gonorrhoeae lipopolysaccharide.

The Synthesis and Identification of a Ciprofloxacin Artificial Antigen

2013 ◽  
Vol 830 ◽  
pp. 314-318
Author(s):  
Long Xu ◽  
Li Xin Zhu ◽  
Ren Rong Liu ◽  
Yan Fan ◽  
Wei Meng
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Sodium Periodate ◽  
Periodate Oxidation ◽  
Uv Absorption ◽  
Absorption Method ◽  
Oxidation Method ◽  
Artificial Antigen

The artificial antigen ciprofloxacin-bovine serum albumin (CPFX-BSA) and ciprofloxacin-ovalbumin (CPFX-OVA) were synthesized by carbodiimide (EDC) method and sodium periodate oxidation method, respectively. These two kinds of antigens were identified by the UV absorption method and the animal immunization test, which showed that they were synthesized successfully. The successful synthesis of ciprofloxacin artificial antigen is important to establish immunoassay of ciprofloxacin.

scholarly journals Production and Purification of IgY antibodies from egg yolk

2020 ◽  
Vol 3 (3) ◽  
pp. 192-195
Author(s):  
Sao Mai Tran Thi ◽  
Oanh Dang Thi ◽  
Huong Dang Thi ◽  
Long Le Thanh ◽  
Lan Huong Nguyen Thi ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Antibody Production ◽  
High Purity ◽  
Bovine Serum ◽  
Significant Contribution ◽  
Polyclonal Antibodies ◽  
Scientific Research ◽  
Egg Yolk ◽  
Egg Yolks

Polyclonal antibodies from vaccinated mammalian have made a significant contribution to scientific research and diagnosis. The fact that recent technologies allow the production of antibodies in egg yolks laid by hens has led to the development of an alternative to antibody production that is less dangerous to animals. The results of this study showed that it is possible to produce a large amount of IgY antibody to bovine serum albumin (BSA), content of about 8 mg/mL of egg yolk emulsion. The anti-BSA IgY antibodies with high purity and anti-BSA activity.

scholarly journals STUDIES ON ANTIBODY PRODUCTION

1964 ◽  
Vol 119 (5) ◽  
pp. 743-760 ◽  
Author(s):  
Guy Sainte-Marie ◽  
Albert H. Coons
Keyword(s):  
Bovine Serum Albumin ◽  
Antibody Response ◽  
Serum Albumin ◽  
Lymph Nodes ◽  
Light Microscopy ◽  
Antibody Production ◽  
Bovine Serum ◽  
Generation Time ◽  
Mitotic Time ◽  
Skin Samples

Cells from lymph nodes of rabbits injected repeatedly with bovine serum albumin were transferred subcutaneously to previously irradiated rabbits, and the recipients were immediately injected with bovine serum albumin. A good antibody response resulted. In a series of such animals killed on successive days, skin samples at sites of cell deposition were removed and examined by immunofluorescence and by light microscopy. In these tissues abundant plasmocytes were found to have multiplied and differentiated in a regular progression from immature, to medium, to mature plasmocytes. During the 6 days of the experiment the small plasmocytes accumulated until they reached 85 per cent of the total plasmocytic population. The mitotic index of the large and medium plasmocytes averaged 11 per cent, implying a generation time of 6.3 hours on the basis of a 1 hour mitotic time. This rate of growth is sufficiently rapid to account for all the plasmocytes on the 6th day as deriving from less than 1 per cent of the population initially transferred. This rate and the orderly progression in the evolution of the plasmocytic population, make it highly improbable that plasmocytes arise from transformation of lymphocytes, but rather indicate that they spring from specific precursors already present among the transferred cells.

Development of competitive ELISA for the detection of bovine serum albumin using single-chain variable fragments

2017 ◽  
Vol 525 ◽  
pp. 89-91 ◽  
Author(s):  
Yuan Liu ◽  
Manman Lin ◽  
Xifeng Zhang ◽  
Xiaodan Hu ◽  
Jieru Lin ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Competitive Elisa ◽  
Single Chain ◽  
Single Chain Variable Fragments
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