Monitoring the wheat straw fermentation process using an electronic nose with pattern recognition methods

Aspergillus fumigatus was grown on chopped wheat straw in a solid state fermentation (SSF) process carried out in constant presence of isolated free water inside the fermentation chamber. The system allowed maintaining a constant vapor pressure inside the fermentor throughout the fermentation process. Crude endoglucanase produced by A. fumigatus under such conditions was more thermostable than previously reported enzymes of the same fungal strain which were produced under different conditions and was also more thermostable than a number of other previously reported endoglucanases as well. Various thermostability parameters were calculated for the crude endoglucanase. Half lives (T1/2) of the enzyme were 6930, 866, and 36 min at 60°C, 70°C, and 80°C, respectively. Enthalpies of activation of denaturation (ΔHD*) were 254.04, 253.96, and 253.88 K J mole−1, at 60°C, 70°C and 80°C, respectively, whereas entropies of activation of denaturation (ΔSD*) and free energy changes of activation of denaturation (ΔGD*) were 406.45, 401.01, and 406.07 J mole−1 K−1 and 118.69, 116.41, and 110.53 K J mole−1 at 60°C, 70°C and 80°C, respectively.

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Research on solid-state fermentation process in a closed environment with multiple interferences

Journal of Physics Conference Series ◽

10.1088/1742-6596/1871/1/012104 ◽

2021 ◽

Vol 1871 (1) ◽

pp. 012104

Author(s):

HuiDan Tan ◽

Peng Zhang

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Fermentation Process ◽

Closed Environment

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Pattern recognition methods for SAW sensor array based electronic nose

2010 IEEE International Conference on Computational Intelligence and Computing Research ◽

10.1109/iccic.2010.5705774 ◽

2010 ◽

Author(s):

S. K. Jha ◽

R. D. S. Yadava

Keyword(s):

Pattern Recognition ◽

Electronic Nose ◽

Sensor Array ◽

Saw Sensor ◽

Pattern Recognition Methods

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Fibre degradation of wheat straw by Pleurotus erygnii under low moisture conditions during solid-state fermentation

Letters in Applied Microbiology ◽

10.1111/lam.13104 ◽

2019 ◽

Vol 68 (2) ◽

pp. 182-187 ◽

Cited By ~ 1

Author(s):

P.W. Baker ◽

A. Charlton ◽

M.D.C. Hale

Keyword(s):

Solid State ◽

Wheat Straw ◽

Solid State Fermentation ◽

Fibre Degradation ◽

Moisture Conditions

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Trametes suaveolens as ligninolytic enzyme producer

Zbornik Matice srpske za prirodne nauke ◽

10.2298/zmspn1324437k ◽

2013 ◽

pp. 437-444 ◽

Cited By ~ 3

Author(s):

Aleksandar Knezevic ◽

Ivan Milovanovic ◽

Mirjana Stajic ◽

Jelena Vukojevic

Keyword(s):

Enzyme Activity ◽

Solid State ◽

Wheat Straw ◽

Solid State Fermentation ◽

Nitrogen Concentration ◽

Pollution Prevention ◽

Ligninolytic Enzyme ◽

Species Selection ◽

Submerged Cultivation ◽

Phenol Red

Species of the genus Trametes represent one of the most efficient lignin-degraders which can be attributed to a well developed ligninolytic enzyme system. Current trends are screening of ability of new species to produce these enzymes, as well as the optimization of conditions for their overproduction. Therefore, the aim of the study was to evaluate the potential of T. suaveolens to synthesize laccase and Mn-oxidizing peroxidases during fermentation of the selected plant raw materials. Level of enzyme activities was measured on 7, 10 and 14th day of submersion, as well as the solid-state fermentation of wheat straw and oak sawdust in the presence of NH4NO3 in previously determined optimal nitrogen concentration of 25 mM. The enzyme activity was determined spectrophotometrically using ABTS and phenol red as the substrates. The highest level of laccase activity (1087.1 U/L) was noted after 7 days of wheat straw solid-state fermentation, while during the submerged cultivation the production of the enzyme was not noted. Submerged cultivation in oak sawdust-enriched medium was the optimal for activity of Mn-dependent peroxidase (1767.7 U/L on day 14) and Mn-independent peroxidase (1113.7 U/L on day 7). Introduction of T. suaveolens to produce ligninolytic enzyme represented the base for further study, as well as the determination of relation between enzyme activity and rate of lignin degradation. It could lead to greater possibility of fungal species selection with high delignification capacity, which could take participation in sustainable production of food, feed, fibres, and energy, environmentally friendly pollution prevention, and bioremediation.

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Solid-state fermentation of paper sludge to obtain spores of the fungus Trichoderma asperellum

The EuroBiotech Journal ◽

10.2478/ebtj-2019-0008 ◽

2019 ◽

Vol 3 (2) ◽

pp. 71-77

Author(s):

Rosa Dorta-Vásquez ◽

Oscar Valbuena ◽

Domenico Pavone-Maniscalco

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Fermentation Process ◽

Dry Mass ◽

Paper Sludge ◽

Ecological Risks ◽

Trichoderma Asperellum ◽

Optimal Conditions ◽

Inoculum Concentration ◽

Negative Environmental Impact

Abstract Paper production generates large quantities of a solid waste known as papermaking sludge (PS), which needs to be handled properly for final disposal. The high amount of this byproduct creates expensive economical costs and induces environmental and ecological risks. Therefore, it is necessary to search uses for PS, in order to reduce the negative environmental impact and to generate a more valuable byproduct. Due to the cellulolytic composition of PS, this work evaluated a solid state fermentation process using it as substrate to obtain spores of the fungus Trichoderma asperellum. Optimal conditions to obtain T. asperellum spores were: 60% water content, 3% (w/w) salts (Nutrisol P® and Nutrisol K®), inoculum concentration at 1x105 spores/g, and pasteurized or sterilized PS. Under these conditions it was possible to obtain 2.37x109 spores/g. T. asperellum spores applied directly to pepper (Capsicum anuum) seeds without PS increased significantly seedling dry mass in greenhouse assays. This work suggests an alternative, economic and abundant substrate for production of T. asperellum spores.

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