Ultrasensitive nuclease activity and inhibition assay using microchip electrophoresis with laser induced fluorescence detection

2016 ◽  
Vol 8 (8) ◽  
pp. 1852-1857 ◽  
Author(s):  
Yingfeng Qin ◽  
Jingjin Zhao ◽  
Yong Huang ◽  
Shuting Li ◽  
Shulin Zhao

A novel microchip electrophoresis method with laser induced fluorescence detection was developed for ultrasensitive detection of nuclease activity and inhibitors.

2021 ◽  
Vol 63 (11) ◽  
pp. 51-55
Author(s):  
Manh Huy Nguyen ◽  
◽  
Hong Anh Duong ◽  
Hung Viet Pham ◽  
◽  
...  

This work presented a capillary electrophoresis method with indirect laser-induced fluorescence detection for the simultaneous determination of phenol and four chlorophenol derivatives. The separation was obtained within 20 minutes with a background electrolyte composed of 5 mM borax and 1 mM fluorescein (pH=9.75), 17 kV of applied voltage, and 120 s of hydrostatic injection. At the optimal conditions, the limit of detections for phenolic compounds was in the range of 0.08-0.23 mg/l, and the RSD data of repeatability and reproducibility were less than 8.0% for both migration times and peak areas. This developed method was applied to analyse concentrations of phenolic compounds in surface water and wastewater samples, with the recoveries ranging from 59.4 to 102.5%.


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