Liposome chaperon in cell-free membrane protein synthesis: one-step preparation of KcsA-integrated liposomes and electrophysiological analysis by the planar bilayer method

2016 ◽  
Vol 4 (2) ◽  
pp. 258-264 ◽  
Author(s):  
M. Ando ◽  
M. Akiyama ◽  
D. Okuno ◽  
M. Hirano ◽  
T. Ide ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Membrane Protein ◽  
Planar Bilayer ◽  
Electrophysiological Analysis ◽  
One Step ◽  
Free Membrane

Chaperoning functions of liposomes were investigated using cell-free membrane protein synthesis.

scholarly journals 3PT125 Liposomes as chaperone in cell-free membrane protein synthesis(The 50th Annual Meeting of the Biophysical Society of Japan)

2012 ◽  
Vol 52 (supplement) ◽  
pp. S162
Author(s):  
Minato Akiyama ◽  
Jun-ichi Yasuoka ◽  
Shin-ichi Sawada ◽  
Kazunari Akiyoshi
Keyword(s):  
Protein Synthesis ◽  
Membrane Protein ◽  
Annual Meeting ◽  
Biophysical Society ◽  
Free Membrane

scholarly journals Proteoliposome Engineering with Cell-Free Membrane Protein Synthesis: Control of Membrane Protein Sorting into Liposomes by Chaperoning Systems

2018 ◽  
Vol 5 (10) ◽  
pp. 1800524 ◽  
Author(s):  
Mitsuru Ando ◽  
Shun Schikula ◽  
Yoshihiro Sasaki ◽  
Kazunari Akiyoshi
Keyword(s):  
Protein Synthesis ◽  
Membrane Protein ◽  
Protein Sorting ◽  
Free Membrane

scholarly journals Fluorescent In Situ Folding Control for Rapid Optimization of Cell-Free Membrane Protein Synthesis

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
pp. e42186 ◽  
Author(s):  
Annika Müller-Lucks ◽  
Sinja Bock ◽  
Binghua Wu ◽  
Eric Beitz
Keyword(s):  
Protein Synthesis ◽  
Membrane Protein ◽  
Free Membrane

scholarly journals Preparation of cationic proteoliposomes using cell-free membrane protein synthesis: the chaperoning effect of cationic liposomes

RSC Advances ◽  
2020 ◽  
Vol 10 (48) ◽  
pp. 28741-28745
Author(s):  
Mitsuru Ando ◽  
Yoshihiro Sasaki ◽  
Kazunari Akiyoshi
Keyword(s):  
Protein Synthesis ◽  
Membrane Protein ◽  
Preparation Method ◽  
Cationic Liposomes ◽  
A Cell ◽  
Free Membrane

The preparation method of cationic proteoliposomes is established using a cell-free membrane protein synthesis in the presence of cationic liposomes.

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