Target-triggered cascade recycling amplification for label-free detection of microRNA and molecular logic operations

2016 ◽  
Vol 52 (2) ◽  
pp. 402-405 ◽  
Author(s):  
Sai Bi ◽  
Jiayan Ye ◽  
Ying Dong ◽  
Haoting Li ◽  
Wei Cao
Keyword(s):  
Logic Gates ◽  
Logic Circuits ◽  
Label Free ◽  
Chemiluminescence Detection ◽  
Molecular Logic ◽  
Label Free Detection ◽  
Molecular Scale ◽  
Ultrahigh Sensitivity ◽  
Recycling Amplification ◽  
Logic Operations

A cascade recycling amplification (CRA) that implements cascade logic circuits with feedback amplification function is developed for label-free chemiluminescence detection of microRNA-122 with an ultrahigh sensitivity of 0.82 fM and excellent specificity, which is applied to construct a series of molecular-scale two-input logic gates by using microRNAs as inputs and CRA products as outputs.

Exonuclease-assisted target recycling amplification for label-free chemiluminescence assay and molecular logic operations

2017 ◽  
Vol 53 (90) ◽  
pp. 12201-12204 ◽  
Author(s):  
Yongcun Yan ◽  
Shuzhen Yue ◽  
Tingting Zhao ◽  
Baoyu Luo ◽  
Sai Bi
Keyword(s):  
Logic Gates ◽  
Label Free ◽  
Chemiluminescence Detection ◽  
Target Recycling ◽  
Chemiluminescence Assay ◽  
Molecular Logic ◽  
Molecular Logic Gates ◽  
Recycling Amplification ◽  
Logic Operations ◽  
Amplification Strategy

A versatile exonuclease-assisted target recycling amplification strategy is demonstrated to achieve label-free chemiluminescence detection of DNA and construction of a series of two-input molecular logic gates.

scholarly journals Molecular logic gates: Encoded Multichromophore Response for Simultaneous Label-Free Detection Small 7/2010

Small ◽  
2010 ◽  
Vol 6 (7) ◽  
pp. NA-NA
Author(s):  
Constantin Pistol ◽  
Vincent Mao ◽  
Viresh Thusu ◽  
Alvin R. Lebeck ◽  
Chris Dwyer
Keyword(s):  
Logic Gates ◽  
Label Free ◽  
Molecular Logic ◽  
Molecular Logic Gates ◽  
Label Free Detection

Target-induced structure switching of DNA for label-free and ultrasensitive electrochemiluminescent detection of proteins

2014 ◽  
Vol 50 (24) ◽  
pp. 3211-3213 ◽  
Author(s):  
Mengli Yang ◽  
Ying Chen ◽  
Yun Xiang ◽  
Ruo Yuan ◽  
Yaqin Chai
Keyword(s):  
Dna Structure ◽  
Label Free ◽  
Switching Strategy ◽  
Highly Sensitive ◽  
Label Free Detection ◽  
Exo Iii ◽  
Recycling Amplification ◽  
Structure Switching ◽  
Induced Structure

Highly sensitive and label-free detection of thrombin is achieved via a target-induced DNA structure switching strategy and Exo III-assisted recycling amplification.

Berberine as a novel light-up i-motif fluorescence ligand and its application in designing molecular logic systems

2016 ◽  
Vol 52 (1) ◽  
pp. 179-182 ◽  
Author(s):  
Lijun Xu ◽  
Shanni Hong ◽  
Na Sun ◽  
Kewei Wang ◽  
Lu Zhou ◽  
...  
Keyword(s):  
Logic Gates ◽  
Label Free ◽  
Molecular Logic ◽  
Free Dna ◽  
Logic Systems

Berberine is reported as a light-up fluorescence ligand for i-motif structures, which enables the development of label-free DNA-based logic gates.

Exonuclease-assisted cascaded recycling amplification for label-free detection ofDNA

2012 ◽  
Vol 48 (7) ◽  
pp. 1018-1020 ◽  
Author(s):  
Sai Bi ◽  
Li Li ◽  
Yangyang Cui
Keyword(s):  
Label Free ◽  
Label Free Detection ◽  
Recycling Amplification

scholarly journals A Label-Free Fluorescent DNA Machine for Sensitive Cyclic Amplification Detection of ATP

Materials ◽  
2018 ◽  
Vol 11 (12) ◽  
pp. 2408
Author(s):  
Jingjing Zhang ◽  
Jialun Han ◽  
Shehong Feng ◽  
Chaoqun Niu ◽  
Chen Liu ◽  
...  
Keyword(s):  
Human Urine ◽  
Limit Of Detection ◽  
Logic Gates ◽  
Fluorescent Label ◽  
Label Free ◽  
Background Signal ◽  
Detection Range ◽  
Signal Suppression ◽  
Dna Machine ◽  
Recycling Amplification

In this study, a target recycled amplification, background signal suppression, label-free fluorescent, enzyme-free deoxyribonucleic acid (DNA) machine was developed for the detection of adenosine triphosphate (ATP) in human urine. ATP and DNA fuel strands (FS) were found to trigger the operation of the DNA machine and lead to the cyclic multiplexing of ATP and the release of single stranded (SS) DNA. Double-stranded DNA (dsDNA) was formed on graphene oxide (GO) from the combination of SS DNA and complementary strands (CS′). These double strands then detached from the surface of the GO and in the process interacted with PicoGreen dye resulting in amplifying fluorescence intensity. The results revealed that the detection range of the DNA machine is from 100 to 600 nM (R2 = 0.99108) with a limit of detection (LOD) of 127.9 pM. A DNA machine circuit and AND-NOT-AND-OR logic gates were successfully constructed, and the strategy was used to detect ATP in human urine. With the advantage of target recycling amplification and GO suppressing background signal without fluorescent label and enzyme, this developed strategy has great potential for sensitive detection of different proteins and small molecules.

Two and three input molecular logic operations mediated by a novel azo-azomethine based chromogenic probe through intramolecular charge transfer processes

2015 ◽  
Vol 39 (3) ◽  
pp. 2081-2089 ◽  
Author(s):  
Khatereh Rezaeian ◽  
Hamid Khanmohammadi
Keyword(s):  
Charge Transfer ◽  
Intramolecular Charge Transfer ◽  
Logic Circuits ◽  
Transfer Processes ◽  
Molecular Logic ◽  
Logic Operations

A novel azo-azomethine based chromogenic receptor has been successfully designed and synthesized for the construction of multifunctional molecular logic circuits.

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