Rational optimization of amber suppressor tRNAs toward efficient incorporation of a non-natural amino acid into protein in a eukaryotic wheat germ extract

2016 ◽  
Vol 14 (9) ◽  
pp. 2671-2678 ◽  
Author(s):  
Atsushi Ogawa ◽  
Yuki Namba ◽  
Mai Gakumasawa
Keyword(s):  
Amino Acid ◽  
Wheat Germ ◽  
Natural Amino Acid ◽  
Wheat Germ Extract ◽  
Amber Suppressor

Amber suppressor tRNAs (sup-tRNAs) were rationally optimized toward efficient incorporation of a non-natural amino acid (AcPhe) into protein in a eukaryotic wheat germ extract.

scholarly journals N-terminal sequences of uteroglobin and its precursor

1979 ◽  
Vol 177 (3) ◽  
pp. 985-988 ◽  
Author(s):  
M Atger ◽  
J C Mercier ◽  
G Haze ◽  
F Fridlansky ◽  
E Milgrom
Keyword(s):  
Amino Acid ◽  
Wheat Germ ◽  
Precursor Protein ◽  
Amino Acid Residues ◽  
Wheat Germ Extract ◽  
Terminal Amino Acid ◽  
Terminal Amino

Translation of uteroglobin mRNA in wheat-germ extract has yielded a precursor protein (pre-uteroglobin) containing an N-terminal extension of 21 amino acid residues. The sequence of this extension and that of the 50 N-terminal amino acid residues of uteroglobin have been determined.

scholarly journals Beneficial Impacts of Incorporating the Non-Natural Amino Acid Azulenyl-Alanine into the Trp-Rich Antimicrobial Peptide buCATHL4B

Biomolecules ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 421
Author(s):  
Areetha R. D’Souza ◽  
Matthew R. Necelis ◽  
Alona Kulesha ◽  
Gregory A. Caputo ◽  
Olga V. Makhlynets
Keyword(s):  
Amino Acid ◽  
Antimicrobial Peptides ◽  
Antimicrobial Peptide ◽  
Mechanism Of Action ◽  
Bactericidal Activity ◽  
Antimicrobial Agents ◽  
Human Cells ◽  
Side Chains ◽  
Natural Amino Acid ◽  
Proteolytic Stability

Antimicrobial peptides (AMPs) present a promising scaffold for the development of potent antimicrobial agents. Substitution of tryptophan by non-natural amino acid Azulenyl-Alanine (AzAla) would allow studying the mechanism of action of AMPs by using unique properties of this amino acid, such as ability to be excited separately from tryptophan in a multi-Trp AMPs and environmental insensitivity. In this work, we investigate the effect of Trp→AzAla substitution in antimicrobial peptide buCATHL4B (contains three Trp side chains). We found that antimicrobial and bactericidal activity of the original peptide was preserved, while cytocompatibility with human cells and proteolytic stability was improved. We envision that AzAla will find applications as a tool for studies of the mechanism of action of AMPs. In addition, incorporation of this non-natural amino acid into AMP sequences could enhance their application properties.

scholarly journals The Amino Acid Sequence of Wheat Germ Cytochrome c

1967 ◽  
Vol 242 (11) ◽  
pp. 2764-2779
Author(s):  
Frits C. Stevens ◽  
A.N. Glazer ◽  
Emil L. Smith
Keyword(s):  
Amino Acid ◽  
Cytochrome C ◽  
Amino Acid Sequence ◽  
Wheat Germ

scholarly journals Structure–activity relationships of natural and non-natural amino acid-based amide and 2-oxoamide inhibitors of human phospholipase A2 enzymes

2008 ◽  
Vol 16 (24) ◽  
pp. 10257-10269 ◽  
Author(s):  
Georgia Antonopoulou ◽  
Efrosini Barbayianni ◽  
Victoria Magrioti ◽  
Naomi Cotton ◽  
Daren Stephens ◽  
...  
Keyword(s):  
Amino Acid ◽  
Phospholipase A2 ◽  
Natural Amino Acid ◽  
Structure Activity Relationships ◽  
Structure Activity
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