Isothermal solid-phase recombinase polymerase amplification on microfluidic digital versatile discs (DVDs)

The proposed device, for massive DNA-based screening in limited-resource settings, comprises a centrifugal platform to perform isothermal solid-phase amplification in microarray format and a digital versatile disc drive to read the results.

Download Full-text

Solid-phase recombinase polymerase amplification using an extremely low concentration of a solution primer for sensitive electrochemical detection of hepatitis B viral DNA

Biosensors and Bioelectronics ◽

10.1016/j.bios.2021.113065 ◽

2021 ◽

Vol 179 ◽

pp. 113065

Author(s):

Andi Muhammad Ichzan ◽

Sang-Hyun Hwang ◽

Hyejin Cho ◽

Chiew San Fang ◽

Seonhwa Park ◽

...

Keyword(s):

Hepatitis B ◽

Electrochemical Detection ◽

Solid Phase ◽

Recombinase Polymerase Amplification ◽

Viral Dna ◽

Low Concentration

Download Full-text

APPLICATION OF MULTIVARIABLE CONTROL DESIGN TO DUAL-MODE DIGITAL VERSATILE DISC DRIVE

Asian Journal of Control ◽

10.1111/j.1934-6093.2000.tb00159.x ◽

2008 ◽

Vol 2 (3) ◽

pp. 204-211 ◽

Cited By ~ 1

Author(s):

Myoung Soo Park ◽

Hee Soo Lee ◽

Seung-Ho Kim ◽

Il-Yeong Roh ◽

Yossi Chait

Keyword(s):

Control Design ◽

Multivariable Control ◽

Dual Mode ◽

Digital Versatile Disc ◽

Disc Drive

Download Full-text

The role of DNA diffusion in solid phase polymerase chain reaction with gel-immobilized primers in planar and capillary microarray format

Biomicrofluidics ◽

10.1063/1.3271461 ◽

2009 ◽

Vol 3 (4) ◽

pp. 044112 ◽

Cited By ~ 3

Author(s):

Alexei L. Drobyshev ◽

Tatiana V. Nasedkina ◽

Natalia V. Zakharova

Keyword(s):

Polymerase Chain Reaction ◽

Solid Phase ◽

Chain Reaction ◽

Dna Diffusion ◽

Polymerase Chain ◽

Microarray Format

Download Full-text

Enhanced solid-phase recombinase polymerase amplification and electrochemical detection

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-017-0269-y ◽

2017 ◽

Vol 409 (12) ◽

pp. 3261-3269 ◽

Cited By ~ 10

Author(s):

Jonathan Sabaté del Río ◽

Ivan Magriñà Lobato ◽

Olena Mayboroda ◽

Ioanis Katakis ◽

Ciara K. O’Sullivan

Keyword(s):

Electrochemical Detection ◽

Solid Phase ◽

Recombinase Polymerase Amplification

Download Full-text

Electrochemical detection of Piscirickettsia salmonis genomic DNA from salmon samples using solid-phase recombinase polymerase amplification

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-016-9639-0 ◽

2016 ◽

Vol 408 (30) ◽

pp. 8611-8620 ◽

Cited By ~ 14

Author(s):

Jonathan Sabaté del Río ◽

Marketa Svobodova ◽

Paulina Bustos ◽

Pablo Conejeros ◽

Ciara K. O’Sullivan

Keyword(s):

Electrochemical Detection ◽

Genomic Dna ◽

Solid Phase ◽

Recombinase Polymerase Amplification ◽

Piscirickettsia Salmonis

Download Full-text

Parallel solid-phase isothermal amplification and detection of multiple DNA targets in microliter-sized wells of a digital versatile disc

Microchimica Acta ◽

10.1007/s00604-016-1745-3 ◽

2016 ◽

Vol 183 (3) ◽

pp. 1195-1202 ◽

Cited By ~ 16

Author(s):

Sara Santiago-Felipe ◽

Luis Antonio Tortajada-Genaro ◽

Rosa Puchades ◽

Ángel Maquieira

Keyword(s):

Solid Phase ◽

Isothermal Amplification ◽

Digital Versatile Disc

Download Full-text

Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow Immunoassay for Rapid Detection of Salmonella in Food

Foods ◽

10.3390/foods9010027 ◽

2019 ◽

Vol 9 (1) ◽

pp. 27 ◽

Cited By ~ 7

Author(s):

Jiali Li ◽

Biao Ma ◽

Jiehong Fang ◽

Antong Zhi ◽

Erjing Chen ◽

...

Keyword(s):

Test Line ◽

Point Of Care ◽

Limited Resource ◽

Test Strip ◽

Lateral Flow ◽

Recombinase Polymerase Amplification ◽

Lateral Flow Immunoassay ◽

Quantitative Detection ◽

Control Line ◽

Test Strip Reader

Salmonella can cause serious foodborne diseases. We have developed a lateral flow immunoassay combined with recombinase polymerase amplification (LFD-RPA) for detection of Salmonella in food. The conserved fragment (fimY) was selected as the target gene. Under an optimal condition (37 °C, 10 min), the sensitivity was 12 colony-forming units (CFU)/mL in a pure culture. Testing with 16 non-Salmonella strains as controls revealed that LFD-RPA was specific to the fimY gene of Salmonella. The established assay could detect Salmonella at concentrations as low as 1.29 × 102 CFU/mL in artificially contaminated samples. This detection was at a slightly higher level than that for a pure bacterial culture. Combined with the test strip reader, the LFD-RPA is a feasible method for quantitative detection of Salmonella based on the test line intensity, which was the ratio for the test line and control line of the reflected light. The method could be a potential point-of-care test in limited resource areas and provides a new approach and technical support for the diagnosis of food safety.

Download Full-text