Enhancement of protein production by microalgae Dunaliella salina under mixotrophic conditions using response surface methodology

RSC Advances ◽  
2015 ◽  
Vol 5 (48) ◽  
pp. 38141-38151 ◽  
Author(s):  
S. Kadkhodaei ◽  
S. Abbasiliasi ◽  
T. J. Shun ◽  
H. R. Fard Masoumi ◽  
M. S. Mohamed ◽  
...  

Schematic diagram of the upstream and downstream factors affecting transgene expression.

BioResources ◽  
2014 ◽  
Vol 9 (2) ◽  
Author(s):  
Youn-Ho Moon ◽  
Jungwoo Yang ◽  
Bon-Cheol Koo ◽  
Jong-Woong An ◽  
Young-Lok Cha ◽  
...  

2021 ◽  
Vol 1036 ◽  
pp. 337-344
Author(s):  
Lin Lin Wang ◽  
Xiang Bin Xu ◽  
Qing Song Zhang ◽  
Shi Yi Li

Filling grouting is effective to control the overlying strata movement and surface subsidence in mining and tunnel engineering.Grouting material is used in grouting reinforcement projects. In this experiment,based on the mechanical properties of grouting materials, Expert Design and response surface methodology (RSM) is used to optimize the mix proportion of grout materials. The sand-fly ash ratio ,water reducing agent addition are researched as variables, and the 3D response surface is established between the factors and the performance indicators. Microstructure explains the mechanism of factors affecting performance indicators.The results show the relationship between factors and performance can be well fitted by RSM.The order of significance on compressive strength and solidification ratio is sand-fly ash ratio>water reducing agent addition and the order of significant on viscosity is water reducing agent addition>sand-fly ash ratio.Response surface methodology accurately describes the functional relationship between the target value and the design variables.Microstructure shows hydration reaction and the secondary hydration reaction of fly ash will be carried out after cement-fly ash mixed, and the addition of fly ash is especially important for grout material properties.


Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3850
Author(s):  
Na Zheng ◽  
Yongfei Ming ◽  
Jianzhi Chu ◽  
Shude Yang ◽  
Guochao Wu ◽  
...  

Sanghuangporus baumii, is a widely used medicinal fungus. The polyphenols extracted from this fungus exert antioxidant, anti-inflammatory, and hypoglycemic effects. In this study, polyphenols from the fruiting bodies of S. baumii were obtained using the deep eutectic solvent (DES) extraction method. The factors affecting the extraction yield were investigated at different conditions. Based on the results from single-factor experiments, response surface methodology was used to optimize the extraction conditions. The scavenging ability of the polyphenols on •OH, DPPH, and ABTS+ was determined. The results showed that the DES system composed of choline chloride and malic acid had the best extraction yield (6.37 mg/g). The optimal extraction parameters for response surface methodology were as follows: 42 min, 58 ℃, 1:34 solid–liquid (mg/mL), and water content of 39%. Under these conditions, the yield of polyphenols was the highest (12.58 mg/g). At 0.30 mg/mL, the scavenging ability of the polyphenols on •OH, DPPH, and ABTS+ was 95.71%, 91.08%, and 85.52%, respectively. Thus, the method using DES was more effective than the conventional method of extracting phenolic compounds from the fruiting bodies of S. baumii. Moreover, the extracted polyphenols exhibited potent antioxidant activity.


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