A smart rhodamine–pyridine conjugate for bioimaging of thiocyanate in living cells

RSC Advances ◽  
2015 ◽  
Vol 5 (125) ◽  
pp. 103350-103357 ◽  
Author(s):  
Sandip Mandal ◽  
Animesh Sahana ◽  
Arnab Banerjee ◽  
Damir A. Safin ◽  
Maria G. Babashkina ◽  
...  
Keyword(s):  
Visible Light ◽  
Living Cells ◽  
Kidney Cells ◽  
Human Embryonic Kidney ◽  
Eye Detection ◽  
Human Embryonic Kidney Cells ◽  
Naked Eye ◽  
Naked Eye Detection

A rhodamine–pyridine conjugate, REDA-2PC, can selectively monitor NCS− in human embryonic kidney cells 293. Visible light excitable probe allows fluorescence and naked eye detection of nanomolar NCS−.

Naked-Eye Detection of Hg(II) Ions by Visible Light-Induced Polymerization Initiated by a Hg(II)-Selective Photoredox Catalyst

2021 ◽  
Author(s):  
Hyungwook Kim ◽  
Young Jae Jung ◽  
Jungkyu K. Lee
Keyword(s):  
Visible Light ◽  
Signal Amplification ◽  
Eye Detection ◽  
Naked Eye ◽  
Turn On ◽  
Novel Strategy ◽  
Amplification Strategy ◽  
Naked Eye Detection

We developed a novel strategy for signal amplification strategy using a visible light-induced photopolymerization, initiated by a selective turn-on photoredox catalyst. As photoredox catalysts, fluorescein derivatives are able to initiate...

Novel fluorescent probes based on rhodamine for naked-eye detection of Fe3+ and their application of imaging in living cells

2015 ◽  
Vol 39 (6) ◽  
pp. 4790-4795 ◽  
Author(s):  
Meipan Yang ◽  
Wenfei Meng ◽  
Qingli Ding ◽  
Na Su ◽  
Xiaojing Liu ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Living Cells ◽  
Eye Detection ◽  
Naked Eye ◽  
Naked Eye Detection

Novel fluorescent probes for Fe3+ and their application of imaging in living cells.

Naked eye detection of glutathione in living cells using rhodamine B-functionalized gold nanoparticles coupled with FRET

2012 ◽  
Vol 92 (1) ◽  
pp. 778-782 ◽  
Author(s):  
Huai-Hong Cai ◽  
Hui Wang ◽  
Jinhui Wang ◽  
Wei Wei ◽  
Pei-Hui Yang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Rhodamine B ◽  
Living Cells ◽  
Eye Detection ◽  
Naked Eye ◽  
Functionalized Gold Nanoparticles ◽  
Naked Eye Detection

A selective purine-based fluorescent chemosensor for the “naked-eye” detection of zinc ions (Zn2+): applications in live cell imaging and test strips

2020 ◽  
Vol 44 (35) ◽  
pp. 15195-15201 ◽  
Author(s):  
Haiyan Xu ◽  
Wei Chen ◽  
Weixia Zhang ◽  
Lixin Ju ◽  
Hongfei Lu
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
High Selectivity ◽  
Living Cells ◽  
Fluorescent Chemosensor ◽  
Zinc Ions ◽  
Eye Detection ◽  
Naked Eye ◽  
Turn On ◽  
Naked Eye Detection

In this work, a novel purine based probe PTAHN was successfully designed and synthesized. PTAHN displayed high selectivity towards Zn2+via turn-on fluorescence. What's more, PTAHN can be proficiently employed for imaging Zn2+ in living cells.

Visible light excitable ON fluorescence and naked eye detection of Cu2+via hydrolysis of rhodamine–thiophene conjugate: human breast cancer cell (MCF7) imaging studies

2014 ◽  
Vol 43 (21) ◽  
pp. 7747 ◽  
Author(s):  
Sangita Adhikari ◽  
Abhijit Ghosh ◽  
Sandip Mandal ◽  
Archya Sengupta ◽  
Ansuman Chattopadhyay ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Visible Light ◽  
Human Breast Cancer ◽  
Human Breast Cancer Cell ◽  
Human Breast ◽  
Eye Detection ◽  
Imaging Studies ◽  
Naked Eye ◽  
Naked Eye Detection ◽  
Hydrolysis Of

scholarly journals Functional Comparison between VP64-dCas9-VP64 and dCas9-VP192 CRISPR Activators in Human Embryonic Kidney Cells

2021 ◽  
Vol 22 (1) ◽  
pp. 397
Author(s):  
Nasir Javaid ◽  
Thuong L. H. Pham ◽  
Sangdun Choi
Keyword(s):  
Protein Level ◽  
Reference Genes ◽  
Mrna Level ◽  
High Specificity ◽  
Kidney Cells ◽  
Human Embryonic Kidney ◽  
Human Embryonic Kidney Cells ◽  
Sox2 Expression

Reversal in the transcriptional status of desired genes has been exploited for multiple research, therapeutic, and biotechnological purposes. CRISPR/dCas9-based activators can activate transcriptionally silenced genes after being guided by gene-specific gRNA(s). Here, we performed a functional comparison between two such activators, VP64-dCas9-VP64 and dCas9-VP192, in human embryonic kidney cells by the concomitant targeting of POU5F1 and SOX2. We found 22- and 6-fold upregulations in the mRNA level of POU5F1 by dCas9-VP192 and VP64-dCas9-VP64, respectively. Likewise, SOX2 was up-regulated 4- and 2-fold using dCas9-VP192 and VP64dCas9VP64, respectively. For the POU5F1 protein level, we observed 3.7- and 2.2-fold increases with dCas9-VP192 and VP64-dCas9-VP64, respectively. Similarly, the SOX2 expression was 2.4- and 2-fold higher with dCas9-VP192 and VP64-dCas9-VP64, respectively. We also confirmed that activation only happened upon co-transfecting an activator plasmid with multiplex gRNA plasmid with a high specificity to the reference genes. Our data revealed that dCas9-VP192 is more efficient than VP64-dCas9-VP64 for activating reference genes.

Naked eye detection and measurement of fluoride concentration in groundwater using novel synthesized receptor

2021 ◽  
Vol 328 ◽  
pp. 112776
Author(s):  
Yogendra Singh Solanki ◽  
Priya Yadav ◽  
Madhu Agarwal ◽  
Ragini Gupta ◽  
Sanjeev Gupta ◽  
...  
Keyword(s):  
Fluoride Concentration ◽  
Eye Detection ◽  
Naked Eye ◽  
Naked Eye Detection
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