Integration of a guided-mode resonance filter with microposts for in-cell protein detection

Assessing levels of neuropeptide Y (NPY) in the human body has many medical uses. Accordingly, we report the quantitative detection of NPY biomarkers applying guided-mode resonance (GMR) biosensor methodology. The label-free sensor operates in the near-infrared spectral region exhibiting distinctive resonance signatures. The interaction of NPY with bioselective molecules on the sensor surface causes spectral shifts that directly identify the binding event without additional processing. In the experiments described here, NPY antibodies are attached to the sensor surface to impart specificity during operation. For the low concentrations of NPY of interest, we apply a sandwich NPY assay in which the sensor-linked anti-NPY molecule binds with NPY that subsequently binds with anti-NPY to close the sandwich. The sandwich assay achieves a detection limit of ~0.1 pM NPY. The photonic sensor methodology applied here enables expeditious high-throughput data acquisition with high sensitivity and specificity. The entire bioreaction is recorded as a function of time, in contrast to label-based methods with single-point detection. The convenient methodology and results reported are significant, as the NPY detection range of 0.1–10 pM demonstrated is useful in important medical circumstances.

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Sensitive metal layer-assisted guided-mode resonance SU8 nanopillar array for label-free optical biosensing

Sensors and Actuators B Chemical ◽

10.1016/j.snb.2015.11.114 ◽

2016 ◽

Vol 226 ◽

pp. 204-210 ◽

Cited By ~ 10

Author(s):

Víctor Canalejas-Tejero ◽

Ana López ◽

Rafael Casquel ◽

Miguel Holgado ◽

Carlos Angulo Barrios

Keyword(s):

Metal Layer ◽

Label Free ◽

Optical Biosensing ◽

Guided Mode ◽

Guided Mode Resonance

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Design and Fabrication of Label-Free Biochip Using a Guided Mode Resonance Filter with Nano Grating Structures by Injection Molding Process

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2011.3277 ◽

2011 ◽

Vol 11 (1) ◽

pp. 417-421 ◽

Cited By ~ 8

Author(s):

E. Cho ◽

B. Kim ◽

S. Choi ◽

J. Han ◽

J. Jin ◽

...

Keyword(s):

Injection Molding ◽

Injection Molding Process ◽

Label Free ◽

Molding Process ◽

Guided Mode ◽

Guided Mode Resonance

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Electrical Detection of Protein Biomarkers Using Nanoneedle Biosensors

MRS Proceedings ◽

10.1557/opl.2012.807 ◽

2012 ◽

Vol 1414 ◽

Cited By ~ 7

Author(s):

Rahim Esfandyarpour ◽

Hesaam Esfandyarpour ◽

Mehdi Javanmard ◽

James S. Harris ◽

Ronald W. Davis

Keyword(s):

Physical Adsorption ◽

Ionic Current ◽

Microfluidic Channel ◽

Protein Detection ◽

High Sensitivity ◽

Receptor Protein ◽

Label Free ◽

Electrical Detection ◽

Protein Biomarkers ◽

Impedance Modulation

Abstract:Here we present the development of an array of electrical nano-biosensors in a microfluidic channel, called Nanoneedle biosensors. Then we present the proof of concept study for protein detection. A Nanoneedle biosensor is a real-time, label-free, direct electrical detection platform, which is capable of high sensitivity detection, measuring the change in ionic current and impedance modulation, due to the presence or reaction of biomolecules such as proteins or nucleic acids. We show that the sensors which have been fabricated and characterized for the protein detection. We have functionalized Nanoneedle biosensors with receptors specific to a target protein using physical adsorption for immobilization. We have used biotinylated bovine serum albumin as the receptor and sterptavidin as the target analyte. The detection of streptavidin binding to the receptor protein is also presented.

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