Ultrasensitive detection of microRNAs using catalytic hairpin assembly coupled with enzymatic repairing amplification

2016 ◽  
Vol 52 (93) ◽  
pp. 13584-13587 ◽  
Author(s):  
Chong-Hua Zhang ◽  
Ying Tang ◽  
Ying-Ying Sheng ◽  
Hui Wang ◽  
Zhan Wu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
Ultrasensitive Detection ◽  
Isothermal Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Technology ◽  
Catalytic Hairpin Assembly ◽  
Mirna Detection

A novel isothermal nucleic acid amplification technology is developed by coupling CHA with enzymatic repairing amplification for sensitive and selective miRNA detection.

Traditional and new applications of HCR in biosensing and biomedicine

The Analyst ◽  
2021 ◽  
Author(s):  
Rong Zhou ◽  
Zhuoer Zeng ◽  
Ruowei Sun ◽  
Wenfang Liu ◽  
Qubo Zhu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Double Helix ◽  
Nucleic Acid Amplification ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Single Stranded Dna ◽  
Isothermal Nucleic Acid Amplification ◽  
Hybridization Event ◽  
Nucleic Acid Amplification Technology ◽  
New Applications

Hybridization chain reaction is a very popular isothermal nucleic acid amplification technology. A single-stranded DNA initiator triggers an alternate hybridization event between two hairpins forming a double helix polymer. Due...

Flap endonuclease-initiated enzymatic repairing amplification for ultrasensitive detection of target nucleic acids

Nanoscale ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 3633-3638 ◽  
Author(s):  
Hyowon Jang ◽  
Chang Yeol Lee ◽  
Seoyoung Lee ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Nucleic Acid Amplification ◽  
Ultrasensitive Detection ◽  
Isothermal Nucleic Acid Amplification ◽  
Amplification Method

A new isothermal nucleic acid amplification method termed FERA (Flap endonuclease-initiated Enzymatic Repairing Amplification) is developed for the ultrasensitive detection of target nucleic acids.

Electricity-free chemical heater for isothermal nucleic acid amplification with applications in COVID-19 home testing

The Analyst ◽  
2021 ◽  
Author(s):  
Rui Jie Li ◽  
Michael G. Mauk ◽  
Youngung Seok ◽  
Haim H. Bau
Keyword(s):  
Nucleic Acid ◽  
Phase Change ◽  
Phase Change Material ◽  
Heat Generation ◽  
Temperature Regulation ◽  
Nucleic Acid Amplification ◽  
Enzymatic Amplification ◽  
Isothermal Nucleic Acid Amplification ◽  
Home Testing ◽  
Change Material

Electricty-free incubation of isothermal enzymatic amplification with a composite comprised of exothermic reactants for heat generation and phase change material for temperature regulation.

scholarly journals Rapid electrostatic DNA enrichment for sensitive detection of Trichomonas vaginalis in clinical urinary samples

2020 ◽  
Vol 12 (8) ◽  
pp. 1085-1093
Author(s):  
Justin M. Rosenbohm ◽  
James M. Robson ◽  
Rishabh Singh ◽  
Rose Lee ◽  
Jane Y. Zhang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Trichomonas Vaginalis ◽  
Nucleic Acid Amplification ◽  
Urine Samples ◽  
Sensitive Detection ◽  
Isothermal Nucleic Acid Amplification

Rapid electrostatic enrichment of DNA from urine samples for improved isothermal nucleic acid amplification-based detection of Trichomonas vaginalis.

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