scholarly journals One-step generation of cell-laden microgels using double emulsion drops with a sacrificial ultra-thin oil shell

Lab on a Chip ◽  
2016 ◽  
Vol 16 (9) ◽  
pp. 1549-1555 ◽  
Author(s):  
Chang-Hyung Choi ◽  
Huanan Wang ◽  
Hyomin Lee ◽  
June Hwan Kim ◽  
Liyuan Zhang ◽  
...  
Keyword(s):  
Double Emulsion ◽  
Sacrificial Template ◽  
Emulsion Drops ◽  
One Step ◽  
Step Generation

We present a one-step microfluidic approach to produce monodisperse cell-laden microgels through the use of double emulsion drops with an ultra-thin oil shell as the sacrificial template.

A simple microfluidic method for one-step encapsulation of reagents with varying concentrations in double emulsion drops for nanoliter-scale reactions and analyses

2017 ◽  
Vol 9 (17) ◽  
pp. 2511-2516 ◽  
Author(s):  
Likai Hou ◽  
Yukun Ren ◽  
Yankai Jia ◽  
Xiaokang Deng ◽  
Zheng Tang ◽  
...  
Keyword(s):  
Double Emulsion ◽  
Oil In Water ◽  
Emulsion Drops ◽  
One Step

This work reports a simple microfluidic method for one-step encapsulation of two reagents with varying concentrations in water-in-oil-in-water (W/O/W) double-emulsion drops.

scholarly journals One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering

Cell ◽  
2013 ◽  
Vol 154 (6) ◽  
pp. 1370-1379 ◽  
Author(s):  
Hui Yang ◽  
Haoyi Wang ◽  
Chikdu S. Shivalila ◽  
Albert W. Cheng ◽  
Linyu Shi ◽  
...  
Keyword(s):  
Genome Engineering ◽  
One Step ◽  
Step Generation

scholarly journals One-step generation of knockout pigs by zygote injection of CRISPR/Cas system

Cell Research ◽  
2014 ◽  
Vol 24 (3) ◽  
pp. 372-375 ◽  
Author(s):  
Tang Hai ◽  
Fei Teng ◽  
Runfa Guo ◽  
Wei Li ◽  
Qi Zhou
Keyword(s):  
One Step ◽  
Step Generation
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