A simple microfluidic method for one-step encapsulation of reagents with varying concentrations in double emulsion drops for nanoliter-scale reactions and analyses

2017 ◽  
Vol 9 (17) ◽  
pp. 2511-2516 ◽  
Author(s):  
Likai Hou ◽  
Yukun Ren ◽  
Yankai Jia ◽  
Xiaokang Deng ◽  
Zheng Tang ◽  
...  
Keyword(s):  
Double Emulsion ◽  
Oil In Water ◽  
Emulsion Drops ◽  
One Step

This work reports a simple microfluidic method for one-step encapsulation of two reagents with varying concentrations in water-in-oil-in-water (W/O/W) double-emulsion drops.

Microfluidic fabrication of vesicles with hybrid lipid/nanoparticle bilayer membranes

Soft Matter ◽  
2019 ◽  
Vol 15 (6) ◽  
pp. 1388-1395 ◽  
Author(s):  
Julie Perrotton ◽  
Rubén Ahijado-Guzmán ◽  
Lara H. Moleiro ◽  
Berta Tinao ◽  
Andrés Guerrero-Martinez ◽  
...  
Keyword(s):  
Double Emulsion ◽  
Bilayer Membranes ◽  
Lipid Nanoparticle ◽  
Oil In Water ◽  
Emulsion Drops

Water-in-oil-in-water double emulsion drops, fabricated using capillary microfluidics, enable the formation of vesicles with hybrid lipid/nanoparticle membranes.

scholarly journals One-step generation of cell-laden microgels using double emulsion drops with a sacrificial ultra-thin oil shell

Lab on a Chip ◽  
2016 ◽  
Vol 16 (9) ◽  
pp. 1549-1555 ◽  
Author(s):  
Chang-Hyung Choi ◽  
Huanan Wang ◽  
Hyomin Lee ◽  
June Hwan Kim ◽  
Liyuan Zhang ◽  
...  
Keyword(s):  
Double Emulsion ◽  
Sacrificial Template ◽  
Emulsion Drops ◽  
One Step ◽  
Step Generation

We present a one-step microfluidic approach to produce monodisperse cell-laden microgels through the use of double emulsion drops with an ultra-thin oil shell as the sacrificial template.

Electrocoalescence of paired droplets encapsulated in double-emulsion drops

Lab on a Chip ◽  
2016 ◽  
Vol 16 (22) ◽  
pp. 4313-4318 ◽  
Author(s):  
Yankan Jia ◽  
Yukun Ren ◽  
Weiyu Liu ◽  
Likai Hou ◽  
Ye Tao ◽  
...  
Keyword(s):  
Electric Field ◽  
Double Emulsion ◽  
On Demand ◽  
Ac Electric Field ◽  
Oil In Water ◽  
Emulsion Drops

We utilize an ac electric field to trigger the on-demand fusion of two aqueous cores inside water-in-oil-in-water (W/O/W) double-emulsion drops.

scholarly journals Generation of Ultra-Thin-Shell Microcapsules Using Osmolarity-Controlled Swelling Method

Micromachines ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 444 ◽  
Author(s):  
Jianhua Guo ◽  
Lihua Hou ◽  
Junpeng Hou ◽  
Jiali Yu ◽  
Qingming Hu
Keyword(s):  
Thin Shell ◽  
Shell Thickness ◽  
Double Emulsion ◽  
Spherical Shape ◽  
Thin Shells ◽  
Biomolecular Sensing ◽  
Oil In Water ◽  
Emulsion Drops ◽  
Long Term Observation

Microcapsules are attractive core-shell configurations for studies of controlled release, biomolecular sensing, artificial microbial environments, and spherical film buckling. However, the production of microcapsules with ultra-thin shells remains a challenge. Here we develop a simple and practical osmolarity-controlled swelling method for the mass production of monodisperse microcapsules with ultra-thin shells via water-in-oil-in-water (W/O/W) double-emulsion drops templating. The size and shell thickness of the double-emulsion drops are precisely tuned by changing the osmotic pressure between the inner cores and the suspending medium, indicating the practicability and effectiveness of this swelling method in tuning the shell thickness of double-emulsion drops and the resultant microcapsules. This method enables the production of microcapsules even with an ultra-thin shell less than hundreds of nanometers, which overcomes the difficulty in producing ultra-thin-shell microcapsules using the classic microfluidic emulsion technologies. In addition, the ultra-thin-shell microcapsules can maintain their intact spherical shape for up to 1 year without rupturing in our long-term observation. We believe that the osmolarity-controlled swelling method will be useful in generating ultra-thin-shell polydimethylsiloxane (PDMS) microcapsules for long-term encapsulation, and for thin film folding, buckling and rupturing investigation.

scholarly journals Microencapsulation of Purple Cactus Pear Fruit (Opuntia ficus indica) Extract by the Combined Method W/O/W Double Emulsion-Spray Drying and Conventional Spray Drying: A Comparative Study

Processes ◽  
2018 ◽  
Vol 6 (10) ◽  
pp. 189 ◽  
Author(s):  
Keren Toledo-Madrid ◽  
Tzayhrí Gallardo-Velázquez ◽  
Guillermo Osorio-Revilla
Keyword(s):  
Spray Drying ◽  
Desirability Function ◽  
Double Emulsion ◽  
Dry Weight ◽  
Antioxidant Compounds ◽  
Pear Fruit ◽  
Cactus Pear ◽  
Opuntia Ficus Indica ◽  
Globule Size ◽  
Oil In Water

The aim of this study was to microencapsulate an optimized extract of purple cactus pear fruit (Opuntia ficus indica), rich in phenolic compounds (PC), betacyanins (BC), and betaxanthins (BX), with antioxidant capacity (AC), by two methodologies: combined water-in-oil-in water double emulsions-spray drying (W/O/W-SP) and conventional spray drying, studying the effect of spray drying (SP) on PC and AC. Optimal extraction conditions for bioactive compounds were: 52 °C, for 30 min, using aqueous ethanol (40%) as the solvent, with a 0.85 desirability function, obtaining 17.39 ± 0.11 mg GAE/gdw (gallic acid equivalents per gram of dry weight) for PC, 0.35 mg BE/gdw (betanin equivalents per gram of dry weight) for BC, and 0.26 mg IE/gdw (indicaxanthin equivalents per gram of dry weight) for BX. The best combination of temperatures for conventional SP and W/O/W-SP was 160–80 °C obtaining the highest retention and encapsulation efficiencies for PC. For conventional SP, results were: 107% and 100% PC and AC retention efficiencies (RE-PC and RE-AC), respectively, with 97% of PC encapsulation efficiency (EE-PC), meanwhile for the W/O/W-SP results were: 78% and 103% RE-PC and RE-AC, respectively, with 70% of EE-PC. Microcapsules obtained with W/O/W-SP maintained their structure and integrity and showed a considerable reduction in globule size in the reconstituted W/O/W emulsions due to the spray drying stress. Despite having lower EE-PC than conventional SP, spray dried W/O/W emulsions seems to be a promising controlled-delivery vehicle for antioxidant compounds.

scholarly journals An Oligoimide Particle as a Pickering Emulsion Stabilizer

Polymers ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1071 ◽  
Author(s):  
Yu-Jin Cho ◽  
Dong-Min Kim ◽  
In-Ho Song ◽  
Ju-Young Choi ◽  
Seung-Won Jin ◽  
...  
Keyword(s):  
Particle Concentration ◽  
Pickering Emulsion ◽  
Pickering Emulsions ◽  
Step Method ◽  
Wetting Properties ◽  
Emulsion Droplets ◽  
Step Procedure ◽  
Oil In Water ◽  
One Step ◽  
Oil Emulsions

A pyromellitic dianhydride (PMDA) and 4,4′-oxydianiline (ODA)-based oligoimide (PMDA-ODA) was synthesized by a one-step procedure using water as a solvent. The PMDA-ODA particles showed excellent partial wetting properties and were stably dispersed in both water and oil phases. A stable dispersion was not obtained with comparison PMDA-ODA particles that were synthesized by a conventional two-step method using an organic solvent. Both oil-in-water and water-in-oil Pickering emulsions were prepared using the oligoimide particles synthesized in water, and the size of the emulsion droplet was controlled based on the oligoimide particle concentration. The oligoimide particles were tested to prepare Pickering emulsions using various kinds of oils. The oil-in-water Pickering emulsions were successfully applied to prepare microcapsules of the emulsion droplets. Our new Pickering emulsion stabilizer has the advantages of easy synthesis, no need for surface modification, and the capability of stabilizing both oil-in-water and water-in-oil emulsions.

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