aqueous droplet
Recently Published Documents


TOTAL DOCUMENTS

61
(FIVE YEARS 6)

H-INDEX

13
(FIVE YEARS 0)

Author(s):  
Kasra Khorsand Kazemi ◽  
Telnaz Zarifi ◽  
Majid Mohseni ◽  
Rakesh Narang ◽  
Kevin Golovin ◽  
...  
Keyword(s):  




Author(s):  
Bin Li ◽  
Xiaohui Dou ◽  
Kai Yu ◽  
Yujie Huang ◽  
Wei Zhang ◽  
...  


Author(s):  
Hui Wang ◽  
Haitao Liu ◽  
Xu Zhang ◽  
Yaqing Wang ◽  
Mengqian Zhao ◽  
...  


Author(s):  
Jayaramulu Kolleboyina ◽  
HANEESH SAINI ◽  
Parashuram Kallem ◽  
Eva Otyepková ◽  
Florian Geyer ◽  
...  

Superhydrophobic MOF-nanosheets assembled on the outside of an aqueous droplet form ‘liquid marbles’. A facile mechanochemical-based synthesis followed by ultrasonication was used to prepare two-dimensional superhydrophobic-oleophilic MOF nanosheets of a...



2021 ◽  
Vol 581 ◽  
pp. 334-349
Author(s):  
Sri Ganesh Subramanian ◽  
Sachin Nair ◽  
Sunando DasGupta


PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243361
Author(s):  
Hirofumi Kurita ◽  
Hirohito Nihonyanagi ◽  
Yuki Watanabe ◽  
Kenta Sugano ◽  
Ryuto Shinozaki ◽  
...  

We have developed a novel methodology for the delivery of cell-impermeable molecules, based on electrical short-circuiting via a water droplet in dielectric oil. When a cell suspension droplet is placed between a pair of electrodes with an intense DC electric field, droplet bouncing and droplet deformation, which results in an instantaneous short-circuit, can be induced, depending on the electric field strength. We have demonstrated successful transfection of various mammalian cells using the short-circuiting; however, the molecular mechanism remains to be elucidated. In this study, flow cytometric assays were performed with Jurkat cells. An aqueous droplet containing Jurkat cells and plasmids carrying fluorescent proteins was treated with droplet bouncing or short-circuiting. The short-circuiting resulted in sufficient cell viability and fluorescent protein expression after 24 hours’ incubation. In contrast, droplet bouncing did not result in successful gene transfection. Transient membrane pore formation was investigated by uptake of a cell-impermeable fluorescence dye YO-PRO-1 and the influx of calcium ions. As a result, short-circuiting increased YO-PRO-1 fluorescence intensity and intracellular calcium ion concentration, but droplet bouncing did not. We also investigated the contribution of endocytosis to the transfection. The pre-treatment of cells with endocytosis inhibitors decreased the efficiency of gene transfection in a concentration-dependent manner. Besides, the use of pH-sensitive dye conjugates indicated the formation of an acidic environment in the endosomes after the short-circuiting. Endocytosis is a possible mechanism for the intracellular delivery of exogenous DNA.



2020 ◽  
Vol 30 (49) ◽  
pp. 2004166
Author(s):  
Xin Huang ◽  
Li Tian ◽  
Zhaoyue Wang ◽  
Jianqiang Zhang ◽  
Yu Suen Chan ◽  
...  


2020 ◽  
Vol 32 (3) ◽  
pp. 032003 ◽  
Author(s):  
B. Majhy ◽  
A. K. Sen


Sign in / Sign up

Export Citation Format

Share Document