scholarly journals Design and synthesis of 5-aminolaevulinic acid/3-hydroxypyridinone conjugates for photodynamic therapy: enhancement of protoporphyrin IX production and photo-toxicity in tumor cells

MedChemComm ◽  
2016 ◽  
Vol 7 (6) ◽  
pp. 1190-1196 ◽  
Author(s):  
Tao Zhou ◽  
Le-Le Shao ◽  
Sinan Battah ◽  
Chun-Feng Zhu ◽  
Robert C. Hider ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Tumor Cells ◽  
Protoporphyrin Ix ◽  
Design And Synthesis ◽  
Aminolaevulinic Acid

A series of ALA-HPO conjugates was prepared. One such conjugate was found to possess high phototoxicity.

scholarly journals Mitotic Catastrophe Induced in HeLa Tumor Cells by Photodynamic Therapy with Methyl-aminolevulinate

2019 ◽  
Vol 20 (5) ◽  
pp. 1229 ◽  
Author(s):  
Marta Mascaraque ◽  
Pablo Delgado-Wicke ◽  
Alejandra Damian ◽  
Silvia Lucena ◽  
Elisa Carrasco ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Tumor Cells ◽  
Protoporphyrin Ix ◽  
Aurora Kinase ◽  
Mitotic Catastrophe ◽  
Mitotic Apparatus ◽  
Human Carcinoma ◽  
Cancer Cell Death ◽  
Multipolar Spindles ◽  
Methyl Aminolevulinate

Photodynamic therapy (PDT) constitutes a cancer treatment modality based on the administration of a photosensitizer, which accumulates in tumor cells. The subsequent irradiation of the tumoral area triggers the formation of reactive oxygen species responsible for cancer cell death. One of the compounds approved in clinical practice is methyl-aminolevulinate (MAL), a protoporphyrin IX (PpIX) precursor intermediate of heme synthesis. We have identified the mitotic catastrophe (MC) process after MAL-PDT in HeLa human carcinoma cells. The fluorescence microscopy revealed that PpIX was located mainly at plasma membrane and lysosomes of HeLa cells, although some fluorescence was also detected at endoplasmic reticulum and Golgi apparatus. Cell blockage at metaphase-anaphase transition was observed 24 h after PDT by phase contrast microscopy and flow cytometry. Mitotic apparatus components evaluation by immunofluorescence and Western blot indicated: multipolar spindles and disorganized chromosomes in the equatorial plate accompanied with dispersion of centromeres and alterations in aurora kinase proteins. The mitotic blockage induced by MAL-PDT resembled that induced by two compounds used in chemotherapy, taxol and nocodazole, both targeting microtubules. The alterations in tumoral cells provided evidence of MC induced by MAL-PDT, resolving mainly by apoptosis, directly or through the formation of multinucleate cells.

The morphological changes in rat bladder after photodynamic therapy with 5-aminolaevulinic acid-induced protoporphyrin IX

2007 ◽  
Vol 86 (1) ◽  
pp. 126-132 ◽  
Author(s):  
S. Grönlund-pakkanen ◽  
T.M. Pakkanen ◽  
M. Talja ◽  
V.-M. Kosma ◽  
M. Ala-opas ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Morphological Changes ◽  
Protoporphyrin Ix ◽  
Aminolaevulinic Acid ◽  
Rat Bladder

scholarly journals Photosensitisation and photodynamic therapy of oesophageal, duodenal, and colorectal tumours using 5 aminolaevulinic acid induced protoporphyrin IX--a pilot study.

Gut ◽  
1995 ◽  
Vol 36 (1) ◽  
pp. 67-75 ◽  
Author(s):  
J Regula ◽  
A J MacRobert ◽  
A Gorchein ◽  
G A Buonaccorsi ◽  
S M Thorpe ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Pilot Study ◽  
Protoporphyrin Ix ◽  
Aminolaevulinic Acid ◽  
Colorectal Tumours

scholarly journals Photodynamic therapy using intravenous δ-aminolaevulinic acid-induced protoporphyrin IX sensitisation in experimental hepatic tumours in rats

1996 ◽  
Vol 74 (10) ◽  
pp. 1526-1533 ◽  
Author(s):  
K Svanberg ◽  
DL Liu ◽  
I Wang ◽  
S Andersson-Engels ◽  
U Stenram ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Protoporphyrin Ix ◽  
Aminolaevulinic Acid ◽  
Hepatic Tumours
Sign in / Sign up

Export Citation Format

Share Document