scholarly journals Block ionomer complexes consisting of siRNA and aRAFT-synthesized hydrophilic-block-cationic copolymers II: the influence of cationic block charge density on gene suppression

2016 ◽  
Vol 7 (39) ◽  
pp. 6044-6054 ◽  
Author(s):  
Keith H. Parsons ◽  
Andrew C. Holley ◽  
Gabrielle A. Munn ◽  
Alex S. Flynt ◽  
Charles L. McCormick
Keyword(s):  
Charge Density ◽  
Cellular Delivery ◽  
Gene Suppression ◽  
Cationic Copolymers ◽  
Block Ionomer Complexes ◽  
Block Ionomer

The effect of cationic block charge density in hydrophilic-block-cationic copolymers on siRNA binding and targeted cellular delivery is investigated.

Block ionomer complexes consisting of siRNA and aRAFT-synthesized hydrophilic-block-cationic copolymers: the influence of cationic block length on gene suppression

2014 ◽  
Vol 5 (24) ◽  
pp. 6967-6976 ◽  
Author(s):  
Andrew C. Holley ◽  
Keith H. Parsons ◽  
Wenming Wan ◽  
Daniel F. Lyons ◽  
G. Reid Bishop ◽  
...  
Keyword(s):  
Block Length ◽  
Gene Suppression ◽  
Cationic Copolymers ◽  
Block Ionomer Complexes ◽  
Block Ionomer

Systematic Evaluation of Protein-Based Nanoparticles for Stable Delivery of Small Interfering RNA

2020 ◽  
Vol 16 (7) ◽  
pp. 1169-1181
Author(s):  
Dhananjay Suresh ◽  
Brian Jenkins ◽  
Ajit Zambre ◽  
Anandhi Upendran ◽  
Raghuraman Kannan
Keyword(s):  
High Stability ◽  
Sirna Delivery ◽  
Systematic Investigation ◽  
Systematic Evaluation ◽  
Cellular Delivery ◽  
Gelatin Nanoparticles ◽  
Gene Suppression ◽  
Protein Levels ◽  
Delivery Vehicles ◽  
Interfering Rna

Developing a delivery vehicle to protect siRNA from degradation is a significant challenge. To solve this challenge, researchers attempted to use protein-based nanoparticles to deliver siRNA with limited to moderate success. However, a systematic investigation of comparing the ability of different protein-based nanoparticles as vehicles to deliver siRNA stably within cells is still unavailable. Therefore, in this study we synthesized a library of both non-targeted (proteinsiRNA) nanoparticles (NPs) and targeted (antibody conjugated protein-siRNA) NPs and evaluated ability to stably deliver siRNA in to cells to silence the gene of interest. We investigated nanoparticles of casein, bovine serum albumin, and gelatin for the delivery of siRNA. We synthesized and characterized a total of 12 nanoconjugates; in these conjugates, we either encapsulated, electrostatically attached, or covalently conjugated siRNA. We evaluated the efficiency of attaching siRNA to nanoconjugates, stability, and cellular delivery. The ability of siRNA to silence the protein of interest in cancer cells was also investigated. Among non-targeted conjugates, BSA matrix imparted relatively high stability to siRNA when encapsulated. Among targeted nanoconjugates, gelatin nanoparticles rendered high stability to siRNA upon covalent conjugation to the surface. On comparing with both targeted and non-targeted NPs for release of siRNA within cells, antibody-gelatin-siRNA conjugate exhibited high release and functional activity (down-regulation of target protein levels) within the cells as confirmed by both fluorescence imaging and Western blotting. In summary, our investigations show that targeted gelatin nanoparticles and non-targeted BSA nanoparticles possess high stability and excellent gene suppression capabilities and warrants further studies. We can extend the results from this study to develop stable siRNA delivery vehicles to specifically silence the protein of interest.

Cross-linked Polymeric Micelles based on Block Ionomer Complexes

2013 ◽  
Vol 23 (4) ◽  
pp. 179-186 ◽  
Author(s):  
Jong Oh Kim ◽  
Thiruganesh Ramasamy ◽  
Chul Soon Yong ◽  
Natalia V. Nukolov ◽  
Tatiana K. Bronich ◽  
...  
Keyword(s):  
Polymeric Micelles ◽  
Block Ionomer Complexes ◽  
Block Ionomer

Advancement in nano pharmaceutical formulation and their biomedical use

2020 ◽  
Vol 10 ◽  
Author(s):  
Dharmendra Kumar ◽  
Rishabha Malviya ◽  
Pramod kumar Sharma ◽  
Akanksha Sharma ◽  
Vineet Bhardwaj
Keyword(s):  
Drug Delivery ◽  
Water Solubility ◽  
Polymeric Micelles ◽  
Medical Sciences ◽  
Organic Nanoparticles ◽  
Drug Delivery Carrier ◽  
Block Ionomer Complexes ◽  
Block Ionomer ◽  
Surface Modified ◽  
Surface Modified Nanoparticles

: Nanoparticles and modified nanoparticles are used in biological and medical sciences as liposomes, polymeric micelles, block ionomer complexes, dendrimers, inorganic and organic nanoparticles. Nanoparticles and surface modified nanoparticles are showed good stability and water solubility and can be used efficiently as drug delivery carrier. This paper summarized the advancement in nanoparticles/surface modified nanoparticles and patents based on them.

Effects of Block Length and Structure of Surfactant on Self-Assembly and Solution Behavior of Block Ionomer Complexes

Langmuir ◽  
2000 ◽  
Vol 16 (2) ◽  
pp. 481-489 ◽  
Author(s):  
Tatiana K. Bronich ◽  
Alexei M. Popov ◽  
Adi Eisenberg ◽  
Victor A. Kabanov ◽  
Alexander V. Kabanov
Keyword(s):  
Self Assembly ◽  
Block Length ◽  
Solution Behavior ◽  
Block Ionomer Complexes ◽  
Block Ionomer

Block Ionomer Complexes with Polystyrene Core-Forming Block in Selective Solvents of Various Polarities. 2. Solution Behavior and Self-Assembly in Nonpolar Solvents

2002 ◽  
Vol 35 (16) ◽  
pp. 6344-6350 ◽  
Author(s):  
Eugene A. Lysenko ◽  
Tatiana K. Bronich ◽  
Elena V. Slonkina ◽  
Adi Eisenberg ◽  
Victor A. Kabanov ◽  
...  
Keyword(s):  
Self Assembly ◽  
Solution Behavior ◽  
Selective Solvents ◽  
Nonpolar Solvents ◽  
Block Ionomer Complexes ◽  
Block Ionomer

Toughening Epoxy Thermosets with Block Ionomer Complexes: A Nanostructure–Mechanical Property Correlation

2012 ◽  
Vol 45 (9) ◽  
pp. 3829-3840 ◽  
Author(s):  
Shuying Wu ◽  
Qipeng Guo ◽  
Shuhua Peng ◽  
Nishar Hameed ◽  
Martin Kraska ◽  
...  
Keyword(s):  
Mechanical Property ◽  
Property Correlation ◽  
Block Ionomer Complexes ◽  
Block Ionomer

Magnetic Block Ionomer Complexes for Potential Dual Imaging and Therapeutic Agents

2012 ◽  
Vol 24 (11) ◽  
pp. 2056-2063 ◽  
Author(s):  
Nikorn Pothayee ◽  
Nipon Pothayee ◽  
Neeta Jain ◽  
Nan Hu ◽  
Sharavanan Balasubramaniam ◽  
...  
Keyword(s):  
Therapeutic Agents ◽  
Block Ionomer Complexes ◽  
Dual Imaging ◽  
Block Ionomer

Block Ionomer Complexes with Polystyrene Core-Forming Block in Selective Solvents of Various Polarities. 1. Solution Behavior and Self-Assembly in Aqueous Media

2002 ◽  
Vol 35 (16) ◽  
pp. 6351-6361 ◽  
Author(s):  
Eugene A. Lysenko ◽  
Tatiana K. Bronich ◽  
Elena V. Slonkina ◽  
Adi Eisenberg ◽  
Victor A. Kabanov ◽  
...  
Keyword(s):  
Self Assembly ◽  
Aqueous Media ◽  
Solution Behavior ◽  
Selective Solvents ◽  
Block Ionomer Complexes ◽  
Block Ionomer

scholarly journals Block ionomer complexes of PEG-block-poly(4-vinylbenzylphosphonate) and cationic surfactants as highly stable, pH responsive drug delivery system

2012 ◽  
Vol 160 (3) ◽  
pp. 486-494 ◽  
Author(s):  
Masao Kamimura ◽  
Jong Oh Kim ◽  
Alexander V. Kabanov ◽  
Tatiana K. Bronich ◽  
Yukio Nagasaki
Keyword(s):  
Drug Delivery ◽  
Drug Delivery System ◽  
Delivery System ◽  
Cationic Surfactants ◽  
Ph Responsive ◽  
Block Ionomer Complexes ◽  
Block Ionomer
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