Enhanced attachment of human mesenchymal stem cells on nanograined titania surfaces

We demonstrate the ability of two tripeptides to promote proliferation and modulate the mechanical properties of human mesenchymal stem cells (hMSCs).

Download Full-text

Physically Crosslinked Nanocomposites from Silicate-Crosslinked PEO: Mechanical Properties and Osteogenic Differentiation of Human Mesenchymal Stem Cells

Macromolecular Bioscience ◽

10.1002/mabi.201100508 ◽

2012 ◽

Vol 12 (6) ◽

pp. 779-793 ◽

Cited By ~ 84

Author(s):

Akhilesh K. Gaharwar ◽

Vipuil Kishore ◽

Christian Rivera ◽

Whitney Bullock ◽

Chia-Jung Wu ◽

...

Keyword(s):

Mechanical Properties ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Osteogenic Differentiation ◽

Human Mesenchymal Stem Cells ◽

Physically Crosslinked

Download Full-text

Injectable biodegradable hydrogels with tunable mechanical properties for the stimulation of neurogenesic differentiation of human mesenchymal stem cells in 3D culture

Biomaterials ◽

10.1016/j.biomaterials.2009.10.042 ◽

2010 ◽

Vol 31 (6) ◽

pp. 1148-1157 ◽

Cited By ~ 182

Author(s):

Li-Shan Wang ◽

Joo Eun Chung ◽

Peggy Pui-Yik Chan ◽

Motoichi Kurisawa

Keyword(s):

Mechanical Properties ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

3D Culture ◽

Human Mesenchymal Stem Cells ◽

Biodegradable Hydrogels ◽

Tunable Mechanical Properties ◽

Stimulation Of

Download Full-text

Thymoquinone loading into hydroxyapatite/alginate scaffolds accelerated the osteogenic differentiation of the mesenchymal stem cells

BioMedical Engineering OnLine ◽

10.1186/s12938-021-00916-1 ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Ebrahim Rahmani-Moghadam ◽

Tahereh Talaei-Khozani ◽

Vahideh Zarrin ◽

Zahra Vojdani

Keyword(s):

Mechanical Properties ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Physical And Mechanical Properties ◽

Stem Cell Differentiation ◽

Calcium Deposition ◽

Differentiation Potential ◽

Mesenchymal Stem Cell Differentiation ◽

Osteogenic Property

Abstract Background Phytochemical agents such as thymoquinone (TQ) have osteogenic property. This study aimed to investigate the synergic impact of TQ and hydroxyapatite on mesenchymal stem cell differentiation. Alginate was also used as drug vehicle. Methods HA scaffolds were fabricated by casting into polyurethane foam and sintering at 800 °C, and then, 1250 °C and impregnated by TQ containing alginate. The adipose-derived stem cells were aliquoted into 4 groups: control, osteogenic induced-, TQ and osteogenic induced- and TQ-treated cultures. Adipose derived-mesenchymal stem cells were mixed with alginate and loaded into the scaffolds Results The results showed that impregnation of HA scaffold with alginate decelerated the degradation rate and reinforced the mechanical strength. TQ loading in alginate/HA had no significant influence on physical and mechanical properties. Real-time RT-PCR showed significant elevation in collagen, osteopontin, and osteocalcin expression at early phase of differentiation. TQ also led to an increase in alkaline phosphatase activity. At long term, TQ administration had no impact on calcium deposition and proliferation rate as well as bone-marker expression. Conclusion TQ accelerates the differentiation of the stem cells into the osteoblasts, without changing the physical and mechanical properties of the scaffolds. TQ also showed a synergic influence on differentiation potential of mesenchymal stem cells.

Download Full-text

Alteration of 3D Matrix Stiffness Regulates Viscoelasticity of Human Mesenchymal Stem Cells

International Journal of Molecular Sciences ◽

10.3390/ijms22052441 ◽

2021 ◽

Vol 22 (5) ◽

pp. 2441

Author(s):

Ting-Wei Kao ◽

Arthur Chiou ◽

Keng-Hui Lin ◽

Yi-Shiuan Liu ◽

Oscar Kuang-Sheng Lee

Keyword(s):

Mechanical Properties ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Mean Square Displacement ◽

Human Mesenchymal Stem Cells ◽

Lineage Specification ◽

Particle Tracking Microrheology ◽

Chemically Induced ◽

3D Matrix ◽

Directing Effect

Human mesenchymal stem cells (hMSCs) possess potential of bone formation and were proposed as ideal material against osteoporosis. Although interrogation of directing effect on lineage specification by physical cues has been proposed, how mechanical stimulation impacts intracellular viscoelasticity during osteogenesis remained enigmatic. Cyto-friendly 3D matrix was prepared with polyacrylamide and conjugated fibronectin. The hMSCs were injected with fluorescent beads and chemically-induced toward osteogenesis. The mechanical properties were assessed using video particle tracking microrheology. Inverted epifluorescence microscope was exploited to capture the Brownian trajectory of hMSCs. Mean square displacement was calculated and transformed into intracellular viscoelasticity. Two different stiffness of microspheres (12 kPa, 1 kPa) were established. A total of 45 cells were assessed. hMSCs possessed equivalent mechanical traits initially in the first week, while cells cultured in rigid matrix displayed significant elevation over elastic (G′) and viscous moduli (G″) on day 7 (p < 0.01) and 14 (p < 0.01). However, after two weeks, soft niches no longer stiffened hMSCs, whereas the effect by rigid substrates was consistently during the entire differentiation course. Stiffness of matrix impacted the viscoelasticity of hMSCs. Detailed recognition of how microenvironment impacts mechanical properties and differentiation of hMSCs will facilitate the advancement of tissue engineering and regenerative medicine.

Download Full-text

Chondroinductive factor-free chondrogenic differentiation of human mesenchymal stem cells in graphene oxide-incorporated hydrogels

Journal of Materials Chemistry B ◽

10.1039/c7tb02172k ◽

2018 ◽

Vol 6 (6) ◽

pp. 908-917 ◽

Cited By ~ 14

Author(s):

He Shen ◽

Hang Lin ◽

Aaron X. Sun ◽

Saijie Song ◽

Zhijun Zhang ◽

...

Keyword(s):

Mechanical Properties ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Graphene Oxide ◽

Stem Cell ◽

Mesenchymal Stem Cell ◽

Chondrogenic Differentiation ◽

Human Mesenchymal Stem Cells

Graphene oxide improves mechanical properties and chondrogenic differentiation state of mesenchymal stem cell-laden, engineered hydrogel constructs, without exogenous chondro-inductive factors.

Download Full-text