A functional oligonucleotide probe from an encapsulated silver nanocluster assembled by rolling circle amplification and its application in label-free sensors

RSC Advances ◽  
2016 ◽  
Vol 6 (92) ◽  
pp. 88967-88973 ◽  
Author(s):  
Yuna Guo ◽  
Yu Wang ◽  
Su Liu ◽  
Jinghua Yu ◽  
Qianqian Pei ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Oligonucleotide Probe ◽  
Low Cost ◽  
Rolling Circle Amplification ◽  
Silver Nanoclusters ◽  
Label Free ◽  
Rolling Circle ◽  
Silver Nanocluster ◽  
E Coli ◽  
Highly Sensitive

A novel label-free, low cost electrochemical biosensor for highly sensitive and selective detection of E. coli has been developed based on rolling circle amplification coupled silver nanoclusters as effective electrochemical probe.

Target-responsive dumbbell probe-mediated rolling circle amplification strategy for highly sensitive Hg2+ detection

RSC Advances ◽  
2014 ◽  
Vol 4 (51) ◽  
pp. 27091-27097 ◽  
Author(s):  
Qingwang Xue ◽  
Yanqin Lv ◽  
Yuanfu Zhang ◽  
Shuling Xu ◽  
Qiaoli Yue ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Selective Detection ◽  
Label Free ◽  
Rolling Circle ◽  
Fluorescent Sensing ◽  
Highly Sensitive ◽  
Mercuric Ions ◽  
Amplification Strategy

A novel label-free amplified fluorescent sensing scheme based on target-responsive dumbbell probe-mediated rolling circle amplification (D-RCA) has been developed for sensitive and selective detection of mercuric ions.

scholarly journals A Label-Free Fluorescent Sensor Based on the Formation of Poly(thymine)-Templated Copper Nanoparticles for the Sensitive and Selective Detection of MicroRNA from Cancer Cells

Chemosensors ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 52
Author(s):  
Qian Ma ◽  
Zhiqiang Gao ◽  
Hiranya Dayal ◽  
Sam Fong Yau Li
Keyword(s):  
Cancer Cells ◽  
Copper Nanoparticles ◽  
Fluorescent Sensor ◽  
Rolling Circle Amplification ◽  
High Specificity ◽  
Excitation Wavelength ◽  
Selective Detection ◽  
Label Free ◽  
Rolling Circle ◽  
Highly Sensitive

In this work, a simple and label-free fluorescence “off” to “on” platform was designed for the sensitive and selective detection of microRNA (miRNA) in cancer cells. This method utilized a padlock DNA-based rolling circle amplification (P-RCA) to synthesize fluorescent poly(thymine) (PolyT) which acted as a template for the synthesis of copper nanoparticles (CuNPs) within 10 minutes under mild conditions. While the repeated PolyT sequence was used as the template for CuNP synthesis, other non-PolyT parts (single strand-DNAs without the capacity to act as the template for CuNP formation) served as “smart glues” or rigid linkers to build complex nanostructures. Under the excitation wavelength of 340 nm, the synthesized CuNPs emitted strong red fluorescence effectively at 620 nm. To demonstrate the use of this method as a universal biosensor platform, lethal-7a (let-7a) miRNA was chosen as the standard target. This sensor could achieve highly sensitive and selective detection of miRNA in the presence of other homologous analogues for the combination of P-RCA with the fluorescent copper nanoparticle. Overall, this novel label-free method holds great potential in the sensitive detection of miRNA with high specificity in real samples.

scholarly journals DNAzyme-Based Target-Triggered Rolling-Circle Amplification for High Sensitivity Detection of microRNAs

Sensors ◽  
2020 ◽  
Vol 20 (7) ◽  
pp. 2017
Author(s):  
Chen Liu ◽  
Jialun Han ◽  
Lujian Zhou ◽  
Jingjing Zhang ◽  
Jie Du
Keyword(s):  
Signal Amplification ◽  
Tumor Suppressor Genes ◽  
Low Cost ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Rolling Circle ◽  
Highly Sensitive ◽  
And Control ◽  
High Sensitivity Detection

MicroRNAs regulate and control the growth and development of cells and can play the role of oncogenes and tumor suppressor genes, which are involved in the occurrence and development of cancers. In this study, DNA fragments obtained by target-induced rolling-circle amplification were constructed to complement with self-cleaving deoxyribozyme (DNAzyme) and release fluorescence biomolecules. This sensing approach can affect multiple signal amplification permitting fluorescence detection of microRNAs at the pmol L−1 level hence affording a simple, highly sensitive, and selective low cost detection platform.

Highly sensitive fluorescent sensor for mercury based on hyperbranched rolling circle amplification

The Analyst ◽  
2015 ◽  
Vol 140 (3) ◽  
pp. 907-911 ◽  
Author(s):  
Jinfeng Chen ◽  
Ping Tong ◽  
Yifen Lin ◽  
Wei Lu ◽  
Yu He ◽  
...  
Keyword(s):  
Fluorescent Sensor ◽  
Rolling Circle Amplification ◽  
Label Free ◽  
Rolling Circle ◽  
Highly Sensitive

A label-free hyperbranched rolling circle amplification (HRCA) based fluorescent sensor has been developed for Hg2+ detection.

Highly sensitive and specific electrochemical biosensor for microRNA-21 detection by coupling catalytic hairpin assembly with rolling circle amplification

The Analyst ◽  
2018 ◽  
Vol 143 (10) ◽  
pp. 2304-2309 ◽  
Author(s):  
Qing Li ◽  
Fanpeng Zeng ◽  
Nan Lyu ◽  
Jun Liang
Keyword(s):  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
Linear Range ◽  
Rolling Circle ◽  
Highly Sensitive ◽  
Catalytic Hairpin Assembly

We combine catalytic hairpin assembly and rolling circle amplification for the detection of microRNA in an electrochemical biosensor with a linear range of 0.5–12 500 pmol and a LOD of 290 fmol.

Rolling circle amplification-mediated in situ synthesis of palladium nanoparticles for the ultrasensitive electrochemical detection of microRNA

The Analyst ◽  
2019 ◽  
Vol 144 (12) ◽  
pp. 3817-3825 ◽  
Author(s):  
Cuiling Zhang ◽  
Dan Li ◽  
Dongwei Li ◽  
Kai Wen ◽  
Xingdong Yang ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Palladium Nanoparticles ◽  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
In Situ Synthesis ◽  
Label Free ◽  
Rolling Circle

An ultrasensitive and label-free electrochemical biosensor for microRNA was developed based on rolling circle amplification-mediated palladium nanoparticles.

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