High nuclearity carbonyl clusters as near-IR contrast agents for photoacoustic in vivo imaging

The ability to detect multiple contrast agents simultaneously would greatly enhance Optical Coherence Tomography (OCT) images, providing nuanced biological context to physiological structures. However, previous OCT contrast agent work has been limited to scenarios where only a single contrast agent could be robustly detected within each voxel. We present a novel spectroscopic technique for de-mixing the spectral signal from multiple OCT contrast agents within a single voxel. We validate our technique in vitro and also demonstrate in vivo imaging of three spectrally distinct gold nanobipyramids, trafficking within the lymphatic system of a live mouse. This approach opens the door to a much broader range of pre-clinical and clinical OCT applications where multiplexed labeling is desirable.

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Study on Establishing Reference Safe Concentrations of MRI Contrast Agents for Optimized Images: Paramagnetic Gd-DTPA-BMEA and Superparamagnetic Ferucarbotran

Applied Sciences ◽

10.3390/app11031165 ◽

2021 ◽

Vol 11 (3) ◽

pp. 1165

Author(s):

Wen-Tien Hsiao ◽

Yi-Hong Chou ◽

Jhong-Wei Tu ◽

Ai-Yih Wang ◽

Lu-Han Lai

Keyword(s):

Contrast Agent ◽

Contrast Agents ◽

Mri Contrast Agents ◽

Mri Contrast Agent ◽

In Vitro Experiments ◽

Mri Contrast ◽

In Vivo Experiments ◽

Contrast Agent Injection

The purpose of this study is to establish the minimal injection doses of magnetic resonance imaging (MRI) contrast agents that can achieve optimized images while improving the safety of injectable MRI drugs. Gadolinium-diethylenetriamine penta-acetic acid (Gd-DTPA) and ferucarbotran, commonly used in clinical practice, were selected and evaluated with in vitro and in vivo experiments. MRI was acquired using T1-weighted (T1W) and T2-weighted (T2W) sequences, and the results were quantitatively analyzed. For in vitro experiments, results showed that T1W and T2W images were optimal when Gd-DTPA-bisamide (2-oxoethyl) (Gd-DTPA-BMEA) and ferucarbotran were diluted to a volume percentage of 0.6% and 0.05%; all comparisons were significant differences in grayscale statistics using one-way analysis of variance (ANOVA). For in vivo experiments, the contrast agent with optimal concentration percentages determined from in vitro experiments were injected into mice with an injection volume of 100 μL, and the images of brain, heart, liver, and mesentery before and after injection were compared. The statistical results showed that the p values of both T1W and T2W were less than 0.001, which were statistically significant. Under safety considerations for MRI contrast agent injection, optimized MRI images could still be obtained after reducing the injection concentration, which can provide a reference for the safety concentrations of MRI contrast agent injection in the future.

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Hybrid Gadolinium Oxide Nanoparticles: Multimodal Contrast Agents for in Vivo Imaging

Journal of the American Chemical Society ◽

10.1021/ja068356j ◽

2007 ◽

Vol 129 (16) ◽

pp. 5076-5084 ◽

Cited By ~ 573

Author(s):

Jean-Luc Bridot ◽

Anne-Charlotte Faure ◽

Sophie Laurent ◽

Charlotte Rivière ◽

Claire Billotey ◽

...

Keyword(s):

Contrast Agents ◽

In Vivo Imaging ◽

Gadolinium Oxide ◽

Oxide Nanoparticles ◽

Gadolinium Oxide Nanoparticles

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Antimonene Nanoflakes as a Photoacoustic Imaging Contrast Agent for Tumor in vivo Imaging

Chinese Journal of Lasers ◽

10.3788/cjl202047.0207033 ◽

2020 ◽

Vol 47 (2) ◽

pp. 0207033

Author(s):

于静文 Yu Jingwen ◽

王秀翃 Wang Xiuhong ◽

冯金超 Feng Jinchao ◽

张娜 Zhang Na ◽

王璞 Wang Pu

Keyword(s):

Contrast Agent ◽

In Vivo Imaging ◽

Photoacoustic Imaging ◽

Imaging Contrast Agent

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Differential structured illumination microendoscopy for in vivo imaging of molecular contrast agents

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1613497113 ◽

2016 ◽

Vol 113 (39) ◽

pp. 10769-10773 ◽

Cited By ~ 16

Author(s):

Pelham Keahey ◽

Preetha Ramalingam ◽

Kathleen Schmeler ◽

Rebecca R. Richards-Kortum

Keyword(s):

Contrast Agents ◽

In Vivo Imaging ◽

Fiber Optic ◽

Adenocarcinoma In Situ ◽

Structured Illumination ◽

Optical Sectioning ◽

Optical Elements ◽

Fiber Optic System ◽

Molecular Contrast

Fiber optic microendoscopy has shown promise for visualization of molecular contrast agents used to study disease in vivo. However, fiber optic microendoscopes have limited optical sectioning capability, and image contrast is limited by out-of-focus light generated in highly scattering tissue. Optical sectioning techniques have been used in microendoscopes to remove out-of-focus light but reduce imaging speed or rely on bulky optical elements that prevent in vivo imaging. Here, we present differential structured illumination microendoscopy (DSIMe), a fiber optic system that can perform structured illumination in real time for optical sectioning without any opto-mechanical components attached to the distal tip of the fiber bundle. We demonstrate the use of DSIMe during in vivo fluorescence imaging in patients undergoing surgery for cervical adenocarcinoma in situ. Images acquired using DSIMe show greater contrast than standard microendoscopy, improving the ability to detect cellular atypia associated with neoplasia.

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PEGylated Gd(OH)3 nanorods as metabolizable contrast agents for computed tomography imaging

New Journal of Chemistry ◽

10.1039/c5nj01980j ◽

2015 ◽

Vol 39 (11) ◽

pp. 8999-9005 ◽

Cited By ~ 4

Author(s):

Yingda Du ◽

Ming Xing ◽

Zhiman Li ◽

Wei Guo

Keyword(s):

Computed Tomography ◽

Contrast Agent ◽

Contrast Agents ◽

Computed Tomography Imaging ◽

Hydrothermal Route ◽

Tomography Imaging

PEGylated Gd(OH)3 nanorods have been efficiently prepared via a facile and green hydrothermal route and used as a metabolizable computed tomography contrast agent for in vivo imaging.

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Targeted tumor MRI with gadobutrol-loaded anti-HER2 immunoliposomes

Acta Radiologica ◽

10.1177/0284185116664225 ◽

2016 ◽

Vol 58 (5) ◽

pp. 573-580 ◽

Cited By ~ 4

Author(s):

Weicui Chen ◽

Bo Liu ◽

Jun Chen ◽

Guoqing Liu ◽

Xian Liu

Keyword(s):

Contrast Agent ◽

Contrast Agents ◽

Signal To Noise Ratio ◽

Cellular Level ◽

Cancer Tissue ◽

Post Injection ◽

Specific Tumor ◽

Noise Ratio ◽

Targeted Contrast Agent

Background Immunoliposomes have been used to deliver MR contrast agents to cancer tissue by targeting tumor associated antigens, thus enabling the visualization of biological processes at the cellular level. Purpose To develop and evaluate the feasibility of specific HER2 targeted liposomal MR contrast agent. Material and Methods Gd-loaded anti-HER2 immunolipomes (Gd-ILs) and non-targeted PEGylated liposomes (Gd-NTLs) were prepared and characterized. Tumor bearing animals were randomized into three groups: Gd- ILs, Gd- NTLs and gadobutrol. Animals were imaged prior and 5, 15, 60, 120 and 180 min after i.v. injection of different contrast agents. The signal intensity enhancement percentage, signal- to- noise ratio and contrast- to –noise ratio was used to qualify tumor enhancement of different groups. After imaging, tumors were excised for histological examination. Results In vivo dynamic MR images, the specific targeted contrast agent bound to tumor tissue and result in a gradual and persisting enhancement for at least 3 hours in mice bearing tumor xenografts, reaching a maximum of 87.7% enhancement after 120 min post-injection. Gd-ILs demonstrated superior tumor enhancement over control non target contrast agent and gadobutrol in HER2 overexpressing tumors at 60, 120 and 180 min post- injection. The SNR and CNR of Gd-ILs in the tumors were significantly greater than that of Gd-NTLs at 60, 120, 180 min post- injection. Conclusion The results indicate the feasibility of Gd-ILs providing prolonged circulation, specific tumor enhancement and cancer cell recognition as targeted contrast agent.

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Optimized Near-IR Fluorescent Agents for in Vivo Imaging of Btk Expression

Bioconjugate Chemistry ◽

10.1021/acs.bioconjchem.5b00152 ◽

2015 ◽

Vol 26 (8) ◽

pp. 1513-1518 ◽

Cited By ~ 34

Author(s):

Eunha Kim ◽

Katherine S. Yang ◽

Rainer H. Kohler ◽

John M. Dubach ◽

Hannes Mikula ◽

...

Keyword(s):

In Vivo Imaging ◽

Near Ir

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Protein MRI contrast agent with unprecedented metal selectivity and sensitivity for liver cancer imaging

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1423021112 ◽

2015 ◽

Vol 112 (21) ◽

pp. 6607-6612 ◽

Cited By ~ 44

Author(s):

Shenghui Xue ◽

Hua Yang ◽

Jingjuan Qiao ◽

Fan Pu ◽

Jie Jiang ◽

...

Keyword(s):

Contrast Agent ◽

Contrast Agents ◽

Mri Contrast Agent ◽

Therapeutic Interventions ◽

Mri Contrast ◽

Liver Cancers ◽

Poor Treatment ◽

Ratio Imaging ◽

Metastatic Liver

With available MRI techniques, primary and metastatic liver cancers that are associated with high mortality rates and poor treatment responses are only diagnosed at late stages, due to the lack of highly sensitive contrast agents without Gd3+ toxicity. We have developed a protein contrast agent (ProCA32) that exhibits high stability for Gd3+ and a 1011-fold greater selectivity for Gd3+ over Zn2+ compared with existing contrast agents. ProCA32, modified from parvalbumin, possesses high relaxivities (r1/r2: 66.8 mmol−1⋅s−1/89.2 mmol−1⋅s−1 per particle). Using T1- and T2-weighted, as well as T2/T1 ratio imaging, we have achieved, for the first time (to our knowledge), robust MRI detection of early liver metastases as small as ∼0.24 mm in diameter, much smaller than the current detection limit of 10–20 mm. Furthermore, ProCA32 exhibits appropriate in vivo preference for liver sinusoidal spaces and pharmacokinetics for high-quality imaging. ProCA32 will be invaluable for noninvasive early detection of primary and metastatic liver cancers as well as for monitoring treatment and guiding therapeutic interventions, including drug delivery.

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