A pH- and temperature-responsive bioresorbable injectable hydrogel based on polypeptide block copolymers for the sustained delivery of proteins in vivo

2018 ◽  
Vol 6 (3) ◽  
pp. 661-671 ◽  
Author(s):  
Md. Hasan Turabee ◽  
Thavasyappan Thambi ◽  
Huu Thuy Trang Duong ◽  
Ji Hoon Jeong ◽  
Doo Sung Lee
Keyword(s):  
Block Copolymers ◽  
Controlled Delivery ◽  
Sustained Delivery ◽  
Injectable Hydrogel ◽  
Temperature Responsive

A pH- and temperature-responsive in situ-forming injectable hydrogel based on comb-type polypeptide block copolymers for the controlled delivery of proteins has been developed.

A Facile Strategy for In Situ Controlled Delivery of Doxorubicin with a pH-Sensitive Injectable Hydrogel

Nano LIFE ◽  
2014 ◽  
Vol 04 (03) ◽  
pp. 1441001 ◽  
Author(s):  
Jun Chen ◽  
Xiaojian Li ◽  
Liqian Gao ◽  
Yi Hu ◽  
Wen Zhong ◽  
...  
Keyword(s):  
Ph Sensitive ◽  
Controlled Delivery ◽  
Cell Uptake ◽  
Injectable Hydrogel ◽  
Poorly Soluble ◽  
Poly Ethylene ◽  
Novel Strategy ◽  
Ph Sensitive Polymer ◽  
Tunable Stiffness

In light of the challenges along with the traditional intravenous administration of chemotherapeutics, injectable hydrogel-drug system emerges as a powerful tool for noninvasive and in situ controlled-release of drugs. Herein, we report a novel strategy of drug delivery system with pH responsive injectable hydrogels by taking advantages of two biomaterials. The first one is a pH sensitive polymer-drug (prodrug) conjugate, poly (ethylene glycol)–doxorubicin (MPEG–DOX) with hydrazone linkage. This prodrug interacted with a second biomaterial, α-cyclodextrin (α-CD) under mild conditions and subsequently formed the hydrogels in minutes with tunable stiffness. The gels showed a sustained release behavior dependent on the surrounding pH and released drugs effectively killed tumor cells (MCF-7). The quick cell uptake and efficient intracellular delivery of DOX were observed under a confocal microscope. This study thus provides a novel and simple drug encapsulation strategy to deliver poorly soluble drugs in situ for a potential targeted chemotherapy.

In-vivo anti-tumor activity of a novel poloxamer-based thermosensitive in situ gel for sustained delivery of norcantharidin

2018 ◽  
Vol 24 (5) ◽  
pp. 623-629 ◽  
Author(s):  
Ming-Hua Xie ◽  
Min Ge ◽  
Jia-Bei Peng ◽  
Xiao-Rui Jiang ◽  
Ding-Sheng Wang ◽  
...  
Keyword(s):  
Sustained Delivery ◽  
In Situ Gel ◽  
Anti Tumor Activity

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

Three-Dimensional Subcellular Organization of Hepatocytes in Intact Liver: Simultaneous Visualization of Cytoskeletal and Membrane Markers by Confocal Microscopy

1996 ◽  
Vol 54 ◽  
pp. 288-289
Author(s):  
Greg V. Martin ◽  
Ann L. Hubbard
Keyword(s):  
Three Dimensional ◽  
Integral Membrane Proteins ◽  
Polarized Secretion ◽  
Microscope Images ◽  
Computer Aided ◽  
Intracellular Organelles ◽  
Cytoskeletal Elements ◽  
Simultaneous Visualization

The microtubule (MT) cytoskeleton is necessary for many of the polarized functions of hepatocytes. Among the functions dependent on the MT-based cytoskeleton are polarized secretion of proteins, delivery of endocytosed material to lysosomes, and transcytosis of integral plasma membrane (PM) proteins. Although microtubules have been shown to be crucial to the establishment and maintenance of functional and structural polarization in the hepatocyte, little is known about the architecture of the hepatocyte MT cytoskeleton in vivo, particularly with regard to its relationship to PM domains and membranous organelles. Using an in situ extraction technique that preserves both microtubules and cellular membranes, we have developed a protocol for immunofluorescent co-localization of cytoskeletal elements and integral membrane proteins within 20 µm cryosections of fixed rat liver. Computer-aided 3D reconstruction of multi-spectral confocal microscope images was used to visualize the spatial relationships among the MT cytoskeleton, PM domains and intracellular organelles.

Sign in / Sign up

Export Citation Format

Share Document