Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture

Haishui Huang; Yin Yu; Yong Hu; Xiaoming He; O. Berk Usta; Martin L. Yarmush

doi:10.1039/c7lc00262a

Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture

Lab on a Chip ◽

10.1039/c7lc00262a ◽

2017 ◽

Vol 17 (11) ◽

pp. 1913-1932 ◽

Cited By ~ 47

Author(s):

Haishui Huang ◽

Yin Yu ◽

Yong Hu ◽

Xiaoming He ◽

O. Berk Usta ◽

...

Keyword(s):

Cell Culture ◽

Mammalian Cell ◽

Critical Review ◽

Mammalian Cell Culture

This critical review discusses microfluidic generation and manipulation of cell-laden hydrogel microcapsules, and their applications for in vitro cell culture.

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Phototoxicity testing In vitro: Evaluation of mammalian cell culture techniques

Food and Chemical Toxicology ◽

10.1016/0278-6915(86)90187-0 ◽

1986 ◽

Vol 24 (6-7) ◽

pp. 789-793 ◽

Cited By ~ 12

Author(s):

S.O. Lock ◽

J.V. Friend

Keyword(s):

Cell Culture ◽

Mammalian Cell ◽

Mammalian Cell Culture ◽

In Vitro Evaluation ◽

Cell Culture Techniques ◽

Culture Techniques

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Perfusion mammalian cell culture for recombinant protein manufacturing – A critical review

Biotechnology Advances ◽

10.1016/j.biotechadv.2018.04.011 ◽

2018 ◽

Vol 36 (4) ◽

pp. 1328-1340 ◽

Cited By ~ 57

Author(s):

Jean-Marc Bielser ◽

Moritz Wolf ◽

Jonathan Souquet ◽

Hervé Broly ◽

Massimo Morbidelli

Keyword(s):

Cell Culture ◽

Recombinant Protein ◽

Mammalian Cell ◽

Critical Review ◽

Mammalian Cell Culture

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Polydimethylsiloxane SlipChip for mammalian cell culture applications

The Analyst ◽

10.1039/c5an00547g ◽

2015 ◽

Vol 140 (21) ◽

pp. 7355-7365 ◽

Cited By ~ 7

Author(s):

Chia-Wen Chang ◽

Chien-Chung Peng ◽

Wei-Hao Liao ◽

Yi-Chung Tung

Keyword(s):

Cell Culture ◽

Mammalian Cell ◽

Mammalian Cell Culture ◽

Multiple Treatment ◽

Cytokine Detection

A polydimethylsiloxane (PDMS) SlipChip for in vitro mammalian cell culture applications, including multiple-treatment assays, cell co-culture, and cytokine detection assays.

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Trypanosoma lewisi: In vitro growth in mammalian cell culture media

Experimental Parasitology ◽

10.1016/0014-4894(72)90113-0 ◽

1972 ◽

Vol 31 (2) ◽

pp. 225-231 ◽

Cited By ~ 13

Author(s):

Janeen Dougherty ◽

Alan S. Rabson ◽

Sandra A. Tyrrell

Keyword(s):

Cell Culture ◽

Mammalian Cell ◽

Culture Media ◽

Mammalian Cell Culture ◽

In Vitro Growth ◽

Cell Culture Media ◽

Trypanosoma Lewisi

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Miniature Bioreactors for Rapid Bioprocess Development of Mammalian Cell Culture

Jurnal Teknologi ◽

10.11113/jt.v59.1569 ◽

2012 ◽

Vol 59 (1) ◽

Author(s):

Mohd Helmi Sani ◽

Frank Baganz

Keyword(s):

Cell Culture ◽

Mammalian Cell ◽

Process Development ◽

Mammalian Cell Culture ◽

Small Scale ◽

Cell Culture Process ◽

Deep Wells ◽

Working Volume ◽

Culture Process ◽

And Control

At present, there are a number of commercial small scale shaken systems available on the market with instrumented controllable microbioreactors such as Micro–24 Microreactor System (Pall Corporation, Port Washington, NY) and M2P Biolector, (M2P Labs GmbH, Aachen, Germany). The Micro–24 system is basically an orbital shaken 24–well plate that operates at working volume 3 – 7 mL with 24 independent reactors (deep wells, shaken and sparged) running simultaneously. Each reactor is designed as single use reactor that has the ability to continuously monitor and control the pH, DO and temperature. The reactor aeration is supplied by sparging air from gas feeds that can be controlled individually. Furthermore, pH can be controlled by gas sparging using either dilute ammonia or carbon dioxide directly into the culture medium through a membrane at the bottom of each reactor. Chen et al., (2009) evaluated the Micro–24 system for the mammalian cell culture process development and found the Micro–24 system is suitable as scaledown tool for cell culture application. The result showed that intra-well reproducibility, cell growth, metabolites profiles and protein titres were scalable with 2 L bioreactors.

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