Thioflavin T specifically brightening “Guanine Island” in duplex-DNA: a novel fluorescent probe for single-nucleotide mutation

The Analyst ◽  
2019 ◽  
Vol 144 (7) ◽  
pp. 2284-2290 ◽  
Author(s):  
Wei Zhou ◽  
Ze Yu ◽  
Ge Ma ◽  
Tian Jin ◽  
Yunchao Li ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Thioflavin T ◽  
Duplex Dna ◽  
Single Nucleotide ◽  
Single Nucleotide Mutation ◽  
Double Stranded Dna ◽  
Nucleotide Mutation

Here, we found that Thioflavin T (ThT) could specifically bind with a G-GGG unit (named as “Guanine Island”) in double stranded DNA (ds-DNA).

Discrimination of Single Nucleotide Mutation by Using a New Class of Immobilized Shared-Stem Double-Stranded DNA Probes

2011 ◽  
Vol 7 (5) ◽  
pp. 640-647 ◽  
Author(s):  
Yunfei Bai ◽  
Dai Lin ◽  
Qin Han ◽  
Yaojuan Jia ◽  
Jing Tu ◽  
...  
Keyword(s):  
Dna Probes ◽  
Single Nucleotide ◽  
New Class ◽  
Single Nucleotide Mutation ◽  
Double Stranded Dna ◽  
Nucleotide Mutation

scholarly journals A Novel Single-Nucleotide Mutation in a CLAVATA3 Gene Homolog Controls a Multilocular Silique Trait in Brassica rapa L.

2014 ◽  
Vol 7 (12) ◽  
pp. 1788-1792 ◽  
Author(s):  
Chuchuan Fan ◽  
Yudi Wu ◽  
Qingyong Yang ◽  
Yang Yang ◽  
Qingwei Meng ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
Single Nucleotide ◽  
Single Nucleotide Mutation ◽  
Nucleotide Mutation

scholarly journals Loss of Heterozygosity and Base Mutation Rates Vary Among Saccharomyces cerevisiae Hybrid Strains

2020 ◽  
Vol 10 (9) ◽  
pp. 3309-3319 ◽  
Author(s):  
Ajith V Pankajam ◽  
Suman Dash ◽  
Asma Saifudeen ◽  
Abhishek Dutta ◽  
Koodali T Nishant
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Loss Of Heterozygosity ◽  
Mutation Rates ◽  
Single Nucleotide ◽  
Single Nucleotide Mutation ◽  
Genome Wide ◽  
Nucleotide Mutation ◽  
Specific Variation ◽  
Chromosomal Changes ◽  
Species Specific

Abstract A growing body of evidence suggests that mutation rates exhibit intra-species specific variation. We estimated genome-wide loss of heterozygosity (LOH), gross chromosomal changes, and single nucleotide mutation rates to determine intra-species specific differences in hybrid and homozygous strains of Saccharomyces cerevisiae. The mutation accumulation lines of the S. cerevisiae hybrid backgrounds - S288c/YJM789 (S/Y) and S288c/RM11-1a (S/R) were analyzed along with the homozygous diploids RM11, S288c, and YJM145. LOH was extensive in both S/Y and S/R hybrid backgrounds. The S/Y background also showed longer LOH tracts, gross chromosomal changes, and aneuploidy. Short copy number aberrations were observed in the S/R background. LOH data from the S/Y and S/R hybrids were used to construct a LOH map for S288c to identify hotspots. Further, we observe up to a sixfold difference in single nucleotide mutation rates among the S. cerevisiae S/Y and S/R genetic backgrounds. Our results demonstrate LOH is common during mitotic divisions in S. cerevisiae hybrids and also highlight genome-wide differences in LOH patterns and rates of single nucleotide mutations between commonly used S. cerevisiae hybrid genetic backgrounds.

Single-nucleotide mutation rate increases close to insertions/deletions in eukaryotes

Nature ◽  
2008 ◽  
Vol 455 (7209) ◽  
pp. 105-108 ◽  
Author(s):  
Dacheng Tian ◽  
Qiang Wang ◽  
Pengfei Zhang ◽  
Hitoshi Araki ◽  
Sihai Yang ◽  
...  
Keyword(s):  
Mutation Rate ◽  
Single Nucleotide ◽  
Single Nucleotide Mutation ◽  
Nucleotide Mutation

scholarly journals A single nucleotide mutation in the mouse renin promoter disrupts blood pressure regulation

2008 ◽  
Author(s):  
Keiji Tanimoto ◽  
Akiko Sugiura ◽  
Sumiyo Kanafusa ◽  
Tomoko Saito ◽  
Naoto Masui ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Blood Pressure Regulation ◽  
Pressure Regulation ◽  
Single Nucleotide ◽  
Single Nucleotide Mutation ◽  
Nucleotide Mutation
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