Dual enzyme-assisted one-step isothermal real-time amplification assay for ultrasensitive detection of polynucleotide kinase activity

2018 ◽  
Vol 54 (98) ◽  
pp. 13841-13844 ◽  
Author(s):  
Xiao-Yu Li ◽  
Yi-Chen Du ◽  
Yan-Nian Pan ◽  
Li-Li Su ◽  
Shuo Shi ◽  
...  
Keyword(s):  
Real Time ◽  
Kinase Activity ◽  
Detection Method ◽  
Ultrasensitive Detection ◽  
Polynucleotide Kinase ◽  
Amplification Assay ◽  
One Step ◽  
Time Amplification

A novel, simple, one-step and one-tube detection method for polynucleotide kinase (PNK) activity based on isothermal real-time amplification assay was proposed.

Label-free and ultrasensitive detection of polynucleotide kinase activity at the single-cell level

2018 ◽  
Vol 54 (13) ◽  
pp. 1583-1586 ◽  
Author(s):  
Meng Liu ◽  
Fei Ma ◽  
Qianyi Zhang ◽  
Chun-yang Zhang
Keyword(s):  
Single Cell ◽  
Kinase Activity ◽  
Fluorescence Method ◽  
Kinase Assay ◽  
Ultrasensitive Detection ◽  
Single Cell Level ◽  
Label Free ◽  
Cell Level ◽  
Polynucleotide Kinase

We develop a label-free fluorescence method for the polynucleotide kinase assay at the single-cell level based on phosphorylation-triggered isothermal exponential amplification.

A modified exponential amplification reaction (EXPAR) with an improved signal-to-noise ratio for ultrasensitive detection of polynucleotide kinase

2019 ◽  
Vol 55 (53) ◽  
pp. 7611-7614 ◽  
Author(s):  
Yu-Peng Zhang ◽  
Yun-Xi Cui ◽  
Xiao-Yu Li ◽  
Yi-Chen Du ◽  
An-Na Tang ◽  
...  
Keyword(s):  
Single Cell ◽  
Kinase Activity ◽  
Signal To Noise Ratio ◽  
Ultrasensitive Detection ◽  
Single Cell Level ◽  
Signal To Noise ◽  
Cell Level ◽  
Polynucleotide Kinase ◽  
Noise Ratio ◽  
Amplification Reaction

We reported a modified exponential amplification reaction strategy and applied it to design an ultrasensitive biosensor for the detection of endogenous polynucleotide kinase activity at single-cell level.

Label-free fluorescence light-up detection of T4 polynucleotide kinase activity using the split-to-intact G-quadruplex strategy by ligation-triggered and toehold-mediated strand displacement release

The Analyst ◽  
2015 ◽  
Vol 140 (16) ◽  
pp. 5450-5453 ◽  
Author(s):  
Lu Zhou ◽  
Xiaoqiang Shen ◽  
Na Sun ◽  
Kewei Wang ◽  
Yuanyuan Zhang ◽  
...  
Keyword(s):  
Kinase Activity ◽  
Detection Method ◽  
Label Free ◽  
Strand Displacement ◽  
Polynucleotide Kinase ◽  
G Quadruplex ◽  
Fluorescence Light ◽  
T4 Polynucleotide Kinase

A label-free, fluorescence light-up detection method for T4 polynucleotide kinase activity has been developed using the split-to-intact G-quadruplex strategy.

scholarly journals A One-Step, Real-Time Reverse Transcription Loopmediated Isothermal Amplification Assay to Detect Potato Virus Y

2015 ◽  
Vol 92 (3) ◽  
pp. 303-311 ◽  
Author(s):  
Agnieszka Przewodowska ◽  
Bogumiła Zacharzewska ◽  
Joanna Chołuj ◽  
Krzysztof Treder
Keyword(s):  
Real Time ◽  
Potato Virus ◽  
Reverse Transcription ◽  
Potato Virus Y ◽  
Isothermal Amplification ◽  
Amplification Assay ◽  
One Step

A Label-Free Bioluminescent Sensor for Real-Time Monitoring Polynucleotide Kinase Activity

2014 ◽  
Vol 86 (16) ◽  
pp. 8481-8488 ◽  
Author(s):  
Jiao Du ◽  
Qinfeng Xu ◽  
Xiaoquan Lu ◽  
Chun-yang Zhang
Keyword(s):  
Real Time ◽  
Kinase Activity ◽  
Label Free ◽  
Real Time Monitoring ◽  
Polynucleotide Kinase

Quencher-free hairpin probes for real-time detection of T4 polynucleotide kinase activity

2016 ◽  
Vol 494 ◽  
pp. 1-3 ◽  
Author(s):  
Changbei Ma ◽  
Haisheng Liu ◽  
Junyan Du ◽  
Hanchun Chen ◽  
Hailun He ◽  
...  
Keyword(s):  
Real Time ◽  
Kinase Activity ◽  
Polynucleotide Kinase ◽  
Hairpin Probes ◽  
Real Time Detection ◽  
T4 Polynucleotide Kinase

Phosphorylation-induced hybridization chain reaction on beads: an ultrasensitive flow cytometric assay for the detection of T4 polynucleotide kinase activity

2015 ◽  
Vol 51 (27) ◽  
pp. 5832-5835 ◽  
Author(s):  
Yuecheng Zhang ◽  
Chenghui Liu ◽  
Sujuan Sun ◽  
Yanli Tang ◽  
Zhengping Li
Keyword(s):  
Kinase Activity ◽  
Hybridization Chain Reaction ◽  
Ultrasensitive Detection ◽  
Flow Cytometric Assay ◽  
Chain Reaction ◽  
Flow Cytometric ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A versatile flow cytometric bead assay (FCBA) has been developed for an ultrasensitive detection of T4 PNK activity.

An Interactive Minicomputer Program for the Indexing and Simulation of Electron Diffraction Patterns

1976 ◽  
Vol 34 ◽  
pp. 542-543
Author(s):  
R.P. Goehner ◽  
W.T. Hatfield ◽  
Prakash Rao
Keyword(s):  
Electron Diffraction ◽  
Real Time ◽  
Pattern Analysis ◽  
Flow Diagram ◽  
Hard Copy ◽  
Time Analysis ◽  
Real Time Analysis ◽  
Transmission Electron ◽  
One Step ◽  
Diffraction Patterns

Computer programs are now available in various laboratories for the indexing and simulation of transmission electron diffraction patterns. Although these programs address themselves to the solution of various aspects of the indexing and simulation process, the ultimate goal is to perform real time diffraction pattern analysis directly off of the imaging screen of the transmission electron microscope. The program to be described in this paper represents one step prior to real time analysis. It involves the combination of two programs, described in an earlier paper(l), into a single program for use on an interactive basis with a minicomputer. In our case, the minicomputer is an INTERDATA 70 equipped with a Tektronix 4010-1 graphical display terminal and hard copy unit.A simplified flow diagram of the combined program, written in Fortran IV, is shown in Figure 1. It consists of two programs INDEX and TEDP which index and simulate electron diffraction patterns respectively. The user has the option of choosing either the indexing or simulating aspects of the combined program.

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