Phosphorylation-induced hybridization chain reaction on beads: an ultrasensitive flow cytometric assay for the detection of T4 polynucleotide kinase activity

2015 ◽  
Vol 51 (27) ◽  
pp. 5832-5835 ◽  
Author(s):  
Yuecheng Zhang ◽  
Chenghui Liu ◽  
Sujuan Sun ◽  
Yanli Tang ◽  
Zhengping Li
Keyword(s):  
Kinase Activity ◽  
Hybridization Chain Reaction ◽  
Ultrasensitive Detection ◽  
Flow Cytometric Assay ◽  
Chain Reaction ◽  
Flow Cytometric ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A versatile flow cytometric bead assay (FCBA) has been developed for an ultrasensitive detection of T4 PNK activity.

A label-free cyclic assembly of G-quadruplex nanowires for cascade amplification detection of T4 polynucleotide kinase activity and inhibition

The Analyst ◽  
2015 ◽  
Vol 140 (17) ◽  
pp. 6124-6130 ◽  
Author(s):  
Zhilu Shi ◽  
Xiafei Zhang ◽  
Rui Cheng ◽  
Baoxin Li ◽  
Yan Jin
Keyword(s):  
Kinase Activity ◽  
Hybridization Chain Reaction ◽  
Label Free ◽  
Chain Reaction ◽  
Polynucleotide Kinase ◽  
G Quadruplex ◽  
Amplification Strategy ◽  
Cascade Amplification ◽  
T4 Polynucleotide Kinase

A label-free and enzyme-free amplification strategy has been developed for sensitively and specifically studying PNK activity and inhibitionviathe hybridization chain reaction (HCR).

Label-free fluorescence assay coupled exonuclease reaction and SYBR Green I for the detection of T4 polynucleotide kinase activity

2020 ◽  
Vol 12 (6) ◽  
pp. 807-812
Author(s):  
Xu Wu ◽  
Shuyi He ◽  
Julia Xiaojun Zhao
Keyword(s):  
Kinase Activity ◽  
Sybr Green ◽  
Fluorescence Assay ◽  
Sybr Green I ◽  
Label Free ◽  
Cleavage Reaction ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A sensitive label-free fluorescence assay for monitoring T4 polynucleotide kinase (T4 PNK) activity and inhibition was developed based on a coupled λ exonuclease cleavage reaction and SYBR Green I.

Label-free and ultrasensitive detection of polynucleotide kinase activity at the single-cell level

2018 ◽  
Vol 54 (13) ◽  
pp. 1583-1586 ◽  
Author(s):  
Meng Liu ◽  
Fei Ma ◽  
Qianyi Zhang ◽  
Chun-yang Zhang
Keyword(s):  
Single Cell ◽  
Kinase Activity ◽  
Fluorescence Method ◽  
Kinase Assay ◽  
Ultrasensitive Detection ◽  
Single Cell Level ◽  
Label Free ◽  
Cell Level ◽  
Polynucleotide Kinase

We develop a label-free fluorescence method for the polynucleotide kinase assay at the single-cell level based on phosphorylation-triggered isothermal exponential amplification.

Dual enzyme-assisted one-step isothermal real-time amplification assay for ultrasensitive detection of polynucleotide kinase activity

2018 ◽  
Vol 54 (98) ◽  
pp. 13841-13844 ◽  
Author(s):  
Xiao-Yu Li ◽  
Yi-Chen Du ◽  
Yan-Nian Pan ◽  
Li-Li Su ◽  
Shuo Shi ◽  
...  
Keyword(s):  
Real Time ◽  
Kinase Activity ◽  
Detection Method ◽  
Ultrasensitive Detection ◽  
Polynucleotide Kinase ◽  
Amplification Assay ◽  
One Step ◽  
Time Amplification

A novel, simple, one-step and one-tube detection method for polynucleotide kinase (PNK) activity based on isothermal real-time amplification assay was proposed.

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