Bio-cleavable nanoprobes for target-triggered catalytic hairpin assembly amplification detection of microRNAs in live cancer cells

Nanoscale ◽  
2018 ◽  
Vol 10 (37) ◽  
pp. 17623-17628 ◽  
Author(s):  
Daxiu Li ◽  
Yulan Wu ◽  
Chunfang Gan ◽  
Ruo Yuan ◽  
Yun Xiang
Keyword(s):  
Cancer Cells ◽  
Highly Sensitive ◽  
Catalytic Hairpin Assembly

Target-triggered assembly of bio-cleavable nanoprobes leads to the highly sensitive imaging of intracellular microRNA-21 in live cancer cells.

scholarly journals Fer and FerT Govern Mitochondrial Susceptibility to Metformin and Hypoxic Stress in Colon and Lung Carcinoma Cells

Cells ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 97
Author(s):  
Odeya Marciano ◽  
Linoy Mehazri ◽  
Sally Shpungin ◽  
Alexander Varvak ◽  
Eldad Zacksenhaus ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Aerobic Glycolysis ◽  
Metastatic Cancer ◽  
Carcinoma Cells ◽  
Metabolic Adaptation ◽  
Hypoxic Stress ◽  
Lung Carcinoma Cells ◽  
Metabolic Role ◽  
Highly Sensitive ◽  
Anticancer Treatment

Aerobic glycolysis is an important metabolic adaptation of cancer cells. However, there is growing evidence that reprogrammed mitochondria also play an important metabolic role in metastatic dissemination. Two constituents of the reprogrammed mitochondria of cancer cells are the intracellular tyrosine kinase Fer and its cancer- and sperm-specific variant, FerT. Here, we show that Fer and FerT control mitochondrial susceptibility to therapeutic and hypoxic stress in metastatic colon (SW620) and non-small cell lung cancer (NSCLC-H1299) cells. Fer- and FerT-deficient SW620 and H1299 cells (SW∆Fer/FerT and H∆Fer/FerT cells, respectively) become highly sensitive to metformin treatment and to hypoxia under glucose-restrictive conditions. Metformin impaired mitochondrial functioning that was accompanied by ATP deficiency and robust death in SW∆Fer/FerT and H∆Fer/FerT cells compared to the parental SW620 and H1299 cells. Notably, selective knockout of the fer gene without affecting FerT expression reduced sensitivity to metformin and hypoxia seen in SW∆Fer/FerT cells. Thus, Fer and FerT modulate the mitochondrial susceptibility of metastatic cancer cells to hypoxia and metformin. Targeting Fer/FerT may therefore provide a novel anticancer treatment by efficient, selective, and more versatile disruption of mitochondrial function in malignant cells.

scholarly journals Highly sensitive and selective detection of PCB 77 using an aptamer-catalytic hairpin assembly in an aquatic environment

RSC Advances ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 5506-5511
Author(s):  
Lin Yuan ◽  
Qiang Fu ◽  
Maojuan Zhou ◽  
Yunqian Ma ◽  
Lihua Zang ◽  
...  
Keyword(s):  
Aquatic Environment ◽  
Selective Detection ◽  
Fluorescence Sensing ◽  
Highly Sensitive ◽  
Catalytic Hairpin Assembly

The fluorescence sensing strategy was used to detect PCB77 based on the aptamer-complex and catalytic hairpin assembly.

scholarly journals Selective and Irreversible Induction of Necroptotic Cell Death in Lung Tumorspheres by Short-Term Exposure to Verapamil in Combination with Sorafenib

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Juan Sebastian Yakisich ◽  
Yogesh Kulkarni ◽  
Neelam Azad ◽  
Anand Krishnan V. Iyer
Keyword(s):  
Cancer Cells ◽  
Culture Conditions ◽  
Serum Starvation ◽  
Adherent Cells ◽  
Key Factors ◽  
Short Term ◽  
Short Term Exposure ◽  
Highly Sensitive ◽  
Free Media ◽  
Drug Free

The presence of highly resistant cancer cells and the toxicity to normal cells are key factors that limit chemotherapy. Here, we used two models of highly resistant lung cancer cells: (1) adherent cells growing under prolonged periods of serum starvation (PPSS) and (2) cells growing as floating tumorspheres (FTs) to evaluate the effect of Verapamil (VP) in combination with Sorafenib (SF). Compared to cells growing under routine culture conditions (RCCs), PPPS cells or FTs were highly sensitive to short-term exposure (24 h) to VP 100 μM + SF 5 μM (VP100 + SF5). Recovery experiments exposing cells to VP100 + SF5 for 24 h followed by incubation in drug-free media for 48 h demonstrated that while PPSS as well as FT cells were unable to recover, cancer cells and the noncancerous cell line Beas-2B growing under RCCs were less sensitive and were also able to recover significantly. VP100 + SF5 induced significant changes in the expression of protein associated with apoptosis, autophagy, and to a lesser extent necroptosis. Coincubation experiments with z-VAD-FMK, necrostatin 1, or chloroquine showed evidence that necroptosis played a central role. Our data demonstrates that highly resistant cancer cells can be selectively eliminated by VP + SF and that necroptosis plays a central role.

A multi-recycling amplification-based sensor for label-free and highly sensitive detection of telomerase from cancer cells

2019 ◽  
Vol 1086 ◽  
pp. 116-121 ◽  
Author(s):  
Xiaoxia Liu ◽  
Xia Li ◽  
Jin Li ◽  
Bingying Jiang ◽  
Ruo Yuan ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Sensitive Detection ◽  
Label Free ◽  
Highly Sensitive ◽  
Recycling Amplification ◽  
Highly Sensitive Detection

scholarly journals PGRMC1-dependent lipophagy promotes ferroptosis in paclitaxel-tolerant persister cancer cells

2021 ◽  
Vol 40 (1) ◽  
Author(s):  
Ji Hyeon You ◽  
Jaewang Lee ◽  
Jong-Lyel Roh
Keyword(s):  
Cancer Cells ◽  
Lipid Droplets ◽  
Tumor Xenograft ◽  
Acid Oxidation ◽  
Mouse Tumor ◽  
Autophagy Induction ◽  
Highly Sensitive ◽  
Xenograft Models

Abstract Background Progesterone receptor membrane component 1 (PGRMC1) is a heme-binding protein inducing dimerization with cytochrome P450, which mediates chemoresistance. Increased PGRMC1 expression is found in multiple types of resistant cancers, but the role of PGRMC1 in the ferroptosis of cancer cells remains unrevealed. Therefore, we examined the role of PGRMC1 in promoting ferroptosis in paclitaxel-tolerant persister cancer cells (PCC). Methods The effects of ferroptosis inducers and PGRMC1 gene silencing/overexpression were tested on head and neck cancer (HNC) cell lines and mouse tumor xenograft models. The results were analyzed about cell viability, death, lipid ROS and iron production, mRNA/protein expression and interaction, and lipid assays. Results PCC had more free fatty acids, lipid droplets, and fatty acid oxidation (FAO) than their parental cells. PCC was highly sensitive to inhibitors of system xc− cystine/glutamate antiporter (xCT), such as erastin, sulfasalazine, and cyst(e)ine deprivation, but less sensitive to (1S,3R)-RSL3. PGRMC1 silencing in PCC reduced ferroptosis sensitivity by xCT inhibitors, and PGRMC1 overexpression in parental cells increased ferroptosis by xCT inhibitors. Lipid droplets were degraded along with autophagy induction and autophagosome formation by erastin treatment in PCC. Lipophagy was accompanied by increased tubulin detyrosination, which was increased by SIRT1 activation but decreased by SIRT1 inhibition. FAO and lipophagy were also promoted by the interaction between lipid droplets and mitochondria. Conclusion PGRMC1 expression increased FAO and ferroptosis sensitivity from in vivo mice experiments. Our data suggest that PGRMC1 promotes ferroptosis by xCT inhibition in PCC.

Metastatic prostate cancer cells are highly sensitive to 3-bromopyruvic acid

Life Sciences ◽  
2019 ◽  
Vol 227 ◽  
pp. 212-223 ◽  
Author(s):  
Monika Pichla ◽  
Jolanta Sroka ◽  
Natalia Pienkowska ◽  
Katarzyna Piwowarczyk ◽  
Zbigniew Madeja ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Metastatic Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Highly Sensitive

Highly Sensitive Electrochemical Detection of Reactive Oxygen Species in Living Cancer Cells Using Monolithic Metallic Foam Electrodes

2020 ◽  
Vol 7 (11) ◽  
pp. 2485-2492
Author(s):  
Yang Yang ◽  
Heng Zhang ◽  
Zhe Wang ◽  
Xuepeng Li ◽  
Abdalla Abdelsamie Abdelrahim Abdelsamie ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cancer Cells ◽  
Electrochemical Detection ◽  
Reactive Oxygen ◽  
Metallic Foam ◽  
Oxygen Species ◽  
Highly Sensitive
Sign in / Sign up

Export Citation Format

Share Document