scholarly journals Vitamin E-based glycoside amphiphiles for membrane protein structural studies

2018 ◽  
Vol 16 (14) ◽  
pp. 2489-2498 ◽  
Author(s):  
Muhammad Ehsan ◽  
Yang Du ◽  
Iago Molist ◽  
Alpay B. Seven ◽  
Parameswaran Hariharan ◽  
...  
Keyword(s):  
Vitamin E ◽  
Membrane Protein ◽  
G Protein ◽  
Structural Studies ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled ◽  
Novel Agent

A vitamin E-based novel agent (i.e., VEG-3) was markedly effective at stabilizing and visualizing a G-protein coupled receptor (GPCR)-Gs complex.

scholarly journals Engineering a G protein-coupled receptor for structural studies: Stabilization of the BLT1 receptor ground state

2009 ◽  
pp. NA-NA
Author(s):  
Aimée Martin ◽  
Marjorie Damian ◽  
Michel Laguerre ◽  
Joseph Parello ◽  
Bernard Pucci ◽  
...  
Keyword(s):  
G Protein ◽  
Ground State ◽  
Structural Studies ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

Structural Studies of Metarhodopsin II, the Activated Form of the G-Protein Coupled Receptor, Rhodopsin†

Biochemistry ◽  
2002 ◽  
Vol 41 (23) ◽  
pp. 7318-7324 ◽  
Author(s):  
Gregory Choi ◽  
Judith Landin ◽  
Jhenny Flor Galan ◽  
Robert R. Birge ◽  
Arlene D. Albert ◽  
...  
Keyword(s):  
G Protein ◽  
Structural Studies ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

scholarly journals Smoothened determines β-arrestin–mediated removal of the G protein–coupled receptor Gpr161 from the primary cilium

2016 ◽  
Vol 212 (7) ◽  
pp. 861-875 ◽  
Author(s):  
Kasturi Pal ◽  
Sun-hee Hwang ◽  
Bandarigoda Somatilaka ◽  
Hemant Badgandi ◽  
Peter K. Jackson ◽  
...  
Keyword(s):  
Membrane Protein ◽  
G Protein ◽  
Primary Cilium ◽  
Protein Composition ◽  
Negative Regulator ◽  
G Protein Coupled Receptor ◽  
New Paradigm ◽  
Subcellular Compartment ◽  
Shh Pathway ◽  
G Protein Coupled

Dynamic changes in membrane protein composition of the primary cilium are central to development and homeostasis, but we know little about mechanisms regulating membrane protein flux. Stimulation of the sonic hedgehog (Shh) pathway in vertebrates results in accumulation and activation of the effector Smoothened within cilia and concomitant disappearance of a negative regulator, the orphan G protein–coupled receptor (GPCR), Gpr161. Here, we describe a two-step process determining removal of Gpr161 from cilia. The first step involves β-arrestin recruitment by the signaling competent receptor, which is facilitated by the GPCR kinase Grk2. An essential factor here is the ciliary trafficking and activation of Smoothened, which by increasing Gpr161–β-arrestin binding promotes Gpr161 removal, both during resting conditions and upon Shh pathway activation. The second step involves clathrin-mediated endocytosis, which functions outside of the ciliary compartment in coordinating Gpr161 removal. Mechanisms determining dynamic compartmentalization of Gpr161 in cilia define a new paradigm for down-regulation of GPCRs during developmental signaling from a specialized subcellular compartment.

G Protein–Coupled Receptor Activation Based on X-ray Structural Studies

2013 ◽  
pp. 877-881
Author(s):  
Dmitry Veprintsev ◽  
Xavier Deupi ◽  
Joerg Standfuss ◽  
Gebhard F. X. Schertler
Keyword(s):  
G Protein ◽  
Receptor Activation ◽  
Structural Studies ◽  
G Protein Coupled Receptor ◽  
X Ray ◽  
G Protein Coupled

Vanadium compounds cause activation of G protein-coupled receptor signaling

2020 ◽  
Author(s):  
Debbie C. Crans ◽  
Duaa Althumairy ◽  
Heide Murakami ◽  
B. George Barisas ◽  
Deborah Roess
Keyword(s):  
G Protein ◽  
Receptor Signaling ◽  
G Protein Coupled Receptor ◽  
Vanadium Compounds ◽  
G Protein Coupled
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