scholarly journals Topology effects on protein–polymer block copolymer self-assembly

2019 ◽  
Vol 10 (14) ◽  
pp. 1751-1761 ◽  
Author(s):  
Takuya Suguri ◽  
Bradley D. Olsen
Keyword(s):  
Block Copolymer ◽  
Self Assembly ◽  
Fluorescent Protein ◽  
Microphase Separation ◽  
Chain Structure ◽  
Synthetic Polymer ◽  
Red Fluorescent Protein ◽  
Protein Polymer ◽  
Ordering Transitions ◽  
Polymer Block

Bioconjugates made of the model red fluorescent protein mCherry and synthetic polymer blocks show that topology, i.e. the BA, BA2, ABA and ABC chain structure of the block copolymers, where B represents the protein and A and C represent polymers, has a significant effect on ordering transitions and the type and size of nanostructures formed during microphase separation.

The Nature of Protein Interactions Governing Globular Protein–Polymer Block Copolymer Self-Assembly

2014 ◽  
Vol 15 (4) ◽  
pp. 1248-1258 ◽  
Author(s):  
Christopher N. Lam ◽  
Minkyu Kim ◽  
Carla S. Thomas ◽  
Dongsook Chang ◽  
Gabriel E. Sanoja ◽  
...  
Keyword(s):  
Block Copolymer ◽  
Protein Interactions ◽  
Self Assembly ◽  
Globular Protein ◽  
Protein Polymer ◽  
Polymer Block

scholarly journals Effect of polymer chemistry on globular protein–polymer block copolymer self-assembly

2014 ◽  
Vol 5 (17) ◽  
pp. 4884-4895 ◽  
Author(s):  
Dongsook Chang ◽  
Christopher N. Lam ◽  
Shengchang Tang ◽  
Bradley D. Olsen
Keyword(s):  
Phase Transitions ◽  
Block Copolymer ◽  
Self Assembly ◽  
Polymer Chemistry ◽  
Protein Polymer ◽  
Polymer Conjugate ◽  
Cubic Phases ◽  
Polymer Interactions ◽  
Nanostructure Control ◽  
Polymer Block

Changing polymer chemistry in protein–polymer conjugate block copolymers results in the formation of previously unobserved cubic phases and changes in protein–polymer interactions that create large shifts in phase transitions, providing a powerful tool for nanostructure control.

scholarly journals Predicting Protein–Polymer Block Copolymer Self-Assembly from Protein Properties

2019 ◽  
Vol 20 (10) ◽  
pp. 3713-3723 ◽  
Author(s):  
Aaron Huang ◽  
Justin M. Paloni ◽  
Amy Wang ◽  
Allie C. Obermeyer ◽  
Hursh V. Sureka ◽  
...  
Keyword(s):  
Block Copolymer ◽  
Self Assembly ◽  
Protein Polymer ◽  
Protein Properties ◽  
Polymer Block

Amphiphilic block copolymer poly(lactic acid)-block -(glycidylazide polymer)-block -polystyrene: synthesis and self-assembly

2017 ◽  
Vol 66 (7) ◽  
pp. 1037-1043 ◽  
Author(s):  
Guoping Li ◽  
Haoxue Dong ◽  
Menghui Liu ◽  
Min Xia ◽  
Chunpeng Chai ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Block Copolymer ◽  
Self Assembly ◽  
Amphiphilic Block Copolymer ◽  
Poly Lactic Acid ◽  
Copolymer Poly ◽  
Polymer Block

scholarly journals Microphase Separation of a PS-b-PFS Block CopolymerviaSolvent Annealing: Effect of Solvent, Substrate, and Exposure Time on Morphology

2015 ◽  
Vol 2015 ◽  
pp. 1-10
Author(s):  
Colm T. O’Mahony ◽  
Dipu Borah ◽  
Michael A. Morris
Keyword(s):  
Block Copolymer ◽  
Self Assembly ◽  
Microphase Separation ◽  
Annealing Effect ◽  
Solvent Annealing ◽  
Spin Casting ◽  
Effect Of Solvent ◽  
Ordered Arrays ◽  
Annealing Conditions ◽  
Nanoscale Features

Block copolymer (BCP) lithography makes use of the microphase separation properties of BCPs to pattern ordered nanoscale features over large areas. This work presents the microphase separation of an asymmetric polystyrene-block-poly(ferrocenyl dimethylsilane) (PS-b-PFS) BCP that allows ordered arrays of nanostructures to be formed by spin casting PS-b-PFS on substrates and subsequent solvent annealing. The effects of the solvent annealing conditions on self-assembly and structural stability are discussed.

Self-assembly of protein-zwitterionic polymer bioconjugates into nanostructured materials

2016 ◽  
Vol 7 (13) ◽  
pp. 2410-2418 ◽  
Author(s):  
Dongsook Chang ◽  
Bradley D. Olsen
Keyword(s):  
Nanostructured Materials ◽  
Self Assembly ◽  
Fluorescent Protein ◽  
Phase Behaviour ◽  
Solution Phase ◽  
Red Fluorescent Protein ◽  
Polymer Backbone ◽  
High Charge Density ◽  
Zwitterionic Polymer ◽  
Concentrated Solution

Bioconjugates of a red fluorescent protein mCherry and a zwitterionic polymer (PDMAPS) are self-assembled into nanostructured materials. The concentrated solution phase behaviour is studied to elucidate the effect of high charge density along the polymer backbone.

Block Copolymers: Thermodynamics, Dynamics, and Small-Angle Scattering

2015 ◽  
Author(s):  
Lee M. Trask ◽  
Nacu Hernandez ◽  
Eric W. Cochran
Keyword(s):  
Block Copolymer ◽  
Block Copolymers ◽  
Small Angle ◽  
Self Assembly ◽  
Microphase Separation ◽  
Small Angle Scattering ◽  
Angle Scattering ◽  
Copolymer Melts ◽  
Shear Processing ◽  
Kinetics Of

This article explores the dynamics, thermodynamics, and small-angle scattering of block copolymers. The goal is to determine what drives the applications of block copolymers, i.e. how block copolymers behave and how they are characterized. The article begins with a summary of the experimental data and various theories that comprise our understanding of block copolymer thermodynamics, with particular emphasis on phase behavior and especially the theory of microphase separation. It then considers topics related to block copolymer dynamics, including diffusion, viscoelasticity and rheology, shear-processing, and the kinetics of self-assembly. It also discusses small-angle scattering techniques as applied to block copolymer characterization, including scattering from ordered block copolymer melts.

Chemically directed self-assembly of perpendicularly aligned cylinders by a liquid crystalline block copolymer

2015 ◽  
Vol 3 (12) ◽  
pp. 2837-2847 ◽  
Author(s):  
N. Yamashita ◽  
S. Watanabe ◽  
K. Nagai ◽  
M. Komura ◽  
T. Iyoda ◽  
...  
Keyword(s):  
Thin Film ◽  
Block Copolymer ◽  
Self Assembly ◽  
Liquid Crystalline ◽  
Microphase Separation ◽  
Thermally Induced ◽  
Multiplication Process

Chemical epitaxy with a density multiplication process was applied to the perpendicularly oriented hexagonal cylinder nanostructure of liquid crystalline block copolymer (PEO-b-PMA(Az)) thin film through thermally induced microphase separation by using a newly designed PMA(Az)24 brush.

scholarly journals Protein charge parameters that influence stability and cellular internalization of polyelectrolyte complex micelles

2022 ◽  
Author(s):  
Rachel Kapelner ◽  
Allie Obermeyer
Keyword(s):  
Block Copolymer ◽  
Protein Delivery ◽  
Mammalian Cells ◽  
Solution Structure ◽  
Polyelectrolyte Complex ◽  
Fluorescent Protein ◽  
Microphase Separation ◽  
Cellular Delivery ◽  
Protein Charge ◽  
Charge Localization

Proteins are an important class of biologics, but there are several recurring challenges to address when designing protein-based therapeutics. These challenges include: the propensity of proteins to aggregate during formulation, relatively low loading in traditional hydrophobic delivery vehicles, and inefficient cellular uptake. This last criterion is particularly challenging for anionic proteins as they cannot cross the anionic plasma membrane. Here we investigated the complex coacervation of anionic proteins with a block copolymer of opposite charge to form polyelectrolyte complex (PEC) micelles for use as a protein delivery vehicle. Using genetically modified variants of the model protein green fluorescent protein (GFP), we evaluated the role of protein charge and charge localization in the formation and stability of PEC micelles. A neutral-cationic block copolymer, POEGMA79-b-qP4VP175, was prepared via RAFT polymerization for complexation and microphase separation with the panel of engineered anionic GFPs. We found that isotropically supercharged proteins formed micelles at higher ionic strength relative to protein variants with charge localized to a polypeptide tag. We then studied GFP delivery by PEC micelles and found that they effectively delivered the protein cargo to mammalian cells. However, cellular delivery varied as a function of protein charge and charge distribution and we found an inverse relationship between the PEC micelle critical salt concentration and delivery efficiency. This model system has highlighted the potential of polyelectrolyte-complexes to deliver anionic proteins intracellularly as well as the importance of correlating solution structure and desired functional activity.

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