Ultrasonically synthesized organic liquid-filled chitosan microcapsules: part 2: characterization using AFM (atomic force microscopy) and combined AFM–confocal laser scanning fluorescence microscopy

Soft Matter ◽  
2018 ◽  
Vol 14 (16) ◽  
pp. 3192-3201 ◽  
Author(s):  
Srinivas Mettu ◽  
Qianyu Ye ◽  
Meifang Zhou ◽  
Raymond Dagastine ◽  
Muthupandian Ashokkumar
Keyword(s):  
Atomic Force Microscopy ◽  
Fluorescence Microscopy ◽  
Young’S Modulus ◽  
Laser Scanning ◽  
Young's Modulus ◽  
Organic Liquid ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Force Microscopy ◽  
Atomic Force

Atomic Force Microscopy (AFM) is used to measure the stiffness and Young's modulus of individual microcapsules that have a chitosan cross-linked shell encapsulating tetradecane.

scholarly journals Исследование влияния колхицина на нативные фибробласты методами атомно-силовой и конфокальной микроскопии

2020 ◽  
Vol 90 (11) ◽  
pp. 1938
Author(s):  
М.М. Халисов ◽  
В.А. Пеннияйнен ◽  
С.А. Подзорова ◽  
К.И. Тимощук ◽  
А.В. Анкудинов ◽  
...  
Keyword(s):  
Young’S Modulus ◽  
Laser Scanning ◽  
Young's Modulus ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Cell Contact ◽  
Force Microscopy ◽  
Atomic Force ◽  
Intracellular Content ◽  
Microscopy Data

Using atomic force and confocal laser scanning microscopy, we studied the effect of colchicine, 1 µg/ml, which is known to cause the depolymerization of tubulin microtubules, on the primary rat fibroblast culture. When analyzing atomic force microscopy data, the sliding type of probe–cell contact was revealed by observing a clear increase of deformation signal at the sample inclined areas. For an unambiguous interpretation of the observed variations in the mechanical characteristics of fibroblasts, it is necessary to prove the sliding of the probe over the cell surface. It was found that some fibroblasts are soft and are characterized by a quite uniform distribution of the apparent Young's modulus over their surface, while others, much harder cells have rigid fibrous structures on the Young's modulus map. Colchicine has been shown to cause significant cell hardening in both groups. Confocal microscopy data show that the observed effect is associated with an increase in the intracellular content of F-actin in fibroblasts.

Characterization of bituminite in Kimmeridge Clay by confocal laser scanning and atomic force microscopy

2022 ◽  
pp. 103927
Author(s):  
Paul C. Hackley ◽  
Jolanta Kus ◽  
João Graciano Mendonça Filho ◽  
Andrew D. Czaja ◽  
Angeles G. Borrego ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Laser Scanning ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Atomic force microscopy combined with confocal laser scanning microscopy: A new look at cells

Bioimaging ◽  
1993 ◽  
Vol 1 (2) ◽  
pp. 63-70 ◽  
Author(s):  
Constant A J Putman ◽  
Anne Marie van Leeuwen ◽  
Bart G de Grooth ◽  
Katarina Rado??ević ◽  
Kees O van der Werf ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Force Microscopy ◽  
Atomic Force ◽  
New Look
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