scholarly journals Исследование влияния колхицина на нативные фибробласты методами атомно-силовой и конфокальной микроскопии

2020 ◽  
Vol 90 (11) ◽  
pp. 1938
Author(s):  
М.М. Халисов ◽  
В.А. Пеннияйнен ◽  
С.А. Подзорова ◽  
К.И. Тимощук ◽  
А.В. Анкудинов ◽  
...  
Keyword(s):  
Young’S Modulus ◽  
Laser Scanning ◽  
Young's Modulus ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Cell Contact ◽  
Force Microscopy ◽  
Atomic Force ◽  
Intracellular Content ◽  
Microscopy Data

Using atomic force and confocal laser scanning microscopy, we studied the effect of colchicine, 1 µg/ml, which is known to cause the depolymerization of tubulin microtubules, on the primary rat fibroblast culture. When analyzing atomic force microscopy data, the sliding type of probe–cell contact was revealed by observing a clear increase of deformation signal at the sample inclined areas. For an unambiguous interpretation of the observed variations in the mechanical characteristics of fibroblasts, it is necessary to prove the sliding of the probe over the cell surface. It was found that some fibroblasts are soft and are characterized by a quite uniform distribution of the apparent Young's modulus over their surface, while others, much harder cells have rigid fibrous structures on the Young's modulus map. Colchicine has been shown to cause significant cell hardening in both groups. Confocal microscopy data show that the observed effect is associated with an increase in the intracellular content of F-actin in fibroblasts.

Ultrasonically synthesized organic liquid-filled chitosan microcapsules: part 2: characterization using AFM (atomic force microscopy) and combined AFM–confocal laser scanning fluorescence microscopy

Soft Matter ◽  
2018 ◽  
Vol 14 (16) ◽  
pp. 3192-3201 ◽  
Author(s):  
Srinivas Mettu ◽  
Qianyu Ye ◽  
Meifang Zhou ◽  
Raymond Dagastine ◽  
Muthupandian Ashokkumar
Keyword(s):  
Atomic Force Microscopy ◽  
Fluorescence Microscopy ◽  
Young’S Modulus ◽  
Laser Scanning ◽  
Young's Modulus ◽  
Organic Liquid ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Force Microscopy ◽  
Atomic Force

Atomic Force Microscopy (AFM) is used to measure the stiffness and Young's modulus of individual microcapsules that have a chitosan cross-linked shell encapsulating tetradecane.

scholarly journals Intragenic Antimicrobial Peptide Hs02 Hampers the Proliferation of Single- and Dual-Species Biofilms of P. aeruginosa and S. aureus: A Promising Agent for Mitigation of Biofilm-Associated Infections

2019 ◽  
Vol 20 (14) ◽  
pp. 3604 ◽  
Author(s):  
Lucinda J. Bessa ◽  
Julia R. Manickchand ◽  
Peter Eaton ◽  
José Roberto S. A. Leite ◽  
Guilherme D. Brand ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Laser Scanning ◽  
Multidrug Resistant ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Antibiofilm Activity ◽  
Bacterial Cells ◽  
Generalized Polarization ◽  
Force Microscopy ◽  
Atomic Force

Pseudomonas aeruginosa and Staphylococcus aureus are two major pathogens involved in a large variety of infections. Their co-occurrence in the same site of infection has been frequently reported and is linked to enhanced virulence and difficulty of treatment. Herein, the antimicrobial and antibiofilm activities of an intragenic antimicrobial peptide (IAP), named Hs02, which was uncovered from the human unconventional myosin 1H protein, were investigated against several P. aeruginosa and S. aureus strains, including multidrug-resistant (MDR) isolates. The antibiofilm activity was evaluated on single- and dual-species biofilms of P. aeruginosa and S. aureus. Moreover, the effect of peptide Hs02 on the membrane fluidity of the strains was assessed through Laurdan generalized polarization (GP). Minimum inhibitory concentration (MIC) values of peptide Hs02 ranged from 2 to 16 μg/mL against all strains and MDR isolates. Though Hs02 was not able to hamper biofilm formation by some strains at sub-MIC values, it clearly affected 24 h preformed biofilms, especially by reducing the viability of the bacterial cells within the single- and dual-species biofilms, as shown by confocal laser scanning microscopy (CLSM) and atomic force microscopy (AFM) images. Laurdan GP values showed that Hs02 induces membrane rigidification in both P. aeruginosa and S. aureus. Peptide Hs02 can potentially be a lead for further improvement as an antibiofilm agent.

scholarly journals Integrated Confocal and Scanning Probe Microscopy for Biomedical Research

2006 ◽  
Vol 6 ◽  
pp. 1609-1618 ◽  
Author(s):  
B.J. Haupt ◽  
A.E. Pelling ◽  
M.A. Horton
Keyword(s):  
Scanning Probe Microscopy ◽  
Laser Scanning ◽  
Fluorescent Imaging ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Pure Material ◽  
Force Microscopy ◽  
Atomic Force ◽  
Ligand Interactions ◽  
Potential Applications

Atomic force microscopy (AFM) continues to be developed, not only in design, but also in application. The new focus of using AFM is changing from pure material to biomedical studies. More frequently, it is being used in combination with other optical imaging methods, such as confocal laser scanning microscopy (CLSM) and fluorescent imaging, to provide a more comprehensive understanding of biological systems. To date, AFM has been used increasingly as a precise micromanipulator, probing and altering the mechanobiological characteristics of living cells and tissues, in order to examine specific, receptor-ligand interactions, material properties, and cell behavior. In this review, we discuss the development of this new hybrid AFM, current research, and potential applications in diagnosis and the detection of disease.

scholarly journals Novel prosthecate bacteria from the candidate phylum Acetothermia revealed by culture-independent genomics and advanced microscopy

2017 ◽  
Author(s):  
Li-Ping Hao ◽  
Simon Jon McIlroy ◽  
Rasmus Hansen Kirkegaard ◽  
Søren Michael Karst ◽  
Warnakulasuriya Eustace Yrosh Fernando ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Metabolic Reconstruction ◽  
Anaerobic Digesters ◽  
Force Microscopy ◽  
Atomic Force ◽  
Culture Independent ◽  
Ecological Importance ◽  
Globally Distributed

AbstractMembers of the candidate phylum Acetothermia are globally distributed and detected in various habitats. However, little is known about their physiology and ecological importance. In this study, an OTU belonging to Acetothermia was detected at high abundance in two full-scale anaerobic digesters. The first closed genome from this phylum was obtained by differential coverage binning of metagenomes and scaffolding with nanopore data. Genome annotation and metabolic reconstruction suggested an anaerobic chemoheterotrophic lifestyle in which the bacterium obtain energy and carbon via fermentation of peptides, amino acids, and simple sugars to acetate, formate, and hydrogen. The morphology was unusual and composed of a central rod-shaped cell with bipolar prosthecae as revealed by fluorescencein situhybridization combined with confocal laser scanning microscopy, Raman microspectroscopy and atomic force microscopy. We hypothesize that these prosthecae allow for increased nutrient uptake by greatly expanding the cell surface area, providing a competitive advantage under nutrient-limited conditions.

scholarly journals Cell Uptake and Validation of Novel PECs for Biomedical Applications

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Ilaria E. Palamà ◽  
Mariarosaria Musarò ◽  
Addolorata M. L. Coluccia ◽  
Stefania D'Amone ◽  
Giuseppe Gigli
Keyword(s):  
Electrostatic Interactions ◽  
Laser Scanning ◽  
Biomedical Application ◽  
Fluorescein Isothiocyanate ◽  
Laser Scanning Microscopy ◽  
Cell Uptake ◽  
Confocal Laser ◽  
Polyelectrolyte Complexes ◽  
Force Microscopy ◽  
Atomic Force

This pilot study provides the proof of principle for biomedical application of novel polyelectrolyte complexes (PECs) obtained via electrostatic interactions between dextran sulphate (DXS) and poly(allylamine hydrochloride) (PAH). Scanning electron microscopy (SEM) and atomic force microscopy (AFM) showed that DXS/PAH polyelectrolyte complexes were Monodispersed with regular rounded-shape features and average diameters of 250 nm at 2 : 1 weight ratios of DXS/PAH. Fluorescently labelled DXS and fluorescein-isothiocyanate- (FITC-)conjugate DXS were used to follow cell uptake efficiency of PECs and biodegradability of their enzymatically degradable DXS-layers by using confocal laser scanning microscopy (CLSM). Moreover, quantitative MTT and Trypan Blue assays were employed to validate PECs as feasible and safe nanoscaled carriers at single-cell level without adverse effects on metabolism and viability.

scholarly journals Atomic force microscopy combined with confocal laser scanning microscopy: A new look at cells

Bioimaging ◽  
1993 ◽  
Vol 1 (2) ◽  
pp. 63-70 ◽  
Author(s):  
Constant A J Putman ◽  
Anne Marie van Leeuwen ◽  
Bart G de Grooth ◽  
Katarina Rado??ević ◽  
Kees O van der Werf ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Force Microscopy ◽  
Atomic Force ◽  
New Look
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