Pentamethine sulfobenzoindocyanine dyes with low net charge states and high photostability

Analogs of NIR dye Cy5.5 displaying excellent photostability have been developed for use in targeted molecular imaging. Here, a probe based on targeting peptide c(RGDyK) illuminates A549 cancer cells in confocal fluorescence microscopy.

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Functional Fluorescence Microscopy Imaging: Quantitative Scanning-Free Confocal Fluorescence Microscopy for the Characterization of Fast Dynamic Processes in Live Cells

Analytical Chemistry ◽

10.1021/acs.analchem.9b01813 ◽

2019 ◽

Vol 91 (17) ◽

pp. 11129-11137 ◽

Cited By ~ 5

Author(s):

Aleksandar J. Krmpot ◽

Stanko N. Nikolić ◽

Sho Oasa ◽

Dimitrios K. Papadopoulos ◽

Marco Vitali ◽

...

Keyword(s):

Fluorescence Microscopy ◽

Confocal Fluorescence Microscopy ◽

Live Cells ◽

Dynamic Processes ◽

Microscopy Imaging ◽

Confocal Fluorescence ◽

Fast Dynamic

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Choline and phosphatidylcholine fluorescent derivatives localization in carcinoma cells studied by laser scanning confocal fluorescence microscopy

European Journal of Cancer ◽

10.1016/j.ejca.2005.02.028 ◽

2005 ◽

Vol 41 (10) ◽

pp. 1453-1459 ◽

Cited By ~ 11

Author(s):

Anna M. Villa ◽

Elvira Caporizzo ◽

Antonio Papagni ◽

Luciano Miozzo ◽

Paola Del Buttero ◽

...

Keyword(s):

Fluorescence Microscopy ◽

Laser Scanning ◽

Carcinoma Cells ◽

Confocal Fluorescence Microscopy ◽

Confocal Fluorescence ◽

Fluorescent Derivatives

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Analysis of Polyhydroxyalkanoates Granules in Haloferax mediterranei by Double-Fluorescence Staining with Nile Red and SYBR Green by Confocal Fluorescence Microscopy

Polymers ◽

10.3390/polym13101582 ◽

2021 ◽

Vol 13 (10) ◽

pp. 1582

Author(s):

Verónica Cánovas ◽

Salvador Garcia-Chumillas ◽

Fuensanta Monzó ◽

Lorena Simó-Cabrera ◽

Carmen Fernández-Ayuso ◽

...

Keyword(s):

Fluorescence Microscopy ◽

Culture Media ◽

Nile Red ◽

Sybr Green ◽

Confocal Fluorescence Microscopy ◽

Fluorescence Staining ◽

Haloferax Mediterranei ◽

Optimized Protocol ◽

Confocal Fluorescence ◽

Pha Production

Haloferaxmediterranei is a haloarchaeon of high interest in biotechnology because it produces and mobilizes intracellular polyhydroxyalkanoate (PHA) granules during growth under stress conditions (limitation of phosphorous in the culture media), among other interesting metabolites (enzymes, carotenoids, etc.). The capability of PHA production by microbes can be monitored with the use of staining-based methods. However, the staining of haloarchaea cells is a challenging task; firstly, due to the high ionic strength of the medium, which is inappropriate for most of dyes, and secondly, due to the low permeability of the haloarchaea S-layer to macromolecules. In this work, Haloferax mediterranei is used as a halophilic archaeon model to describe an optimized protocol for the visualization and analysis of intracellular PHA granules in living cells. The method is based on double-fluorescence staining using Nile red and SYBR Green by confocal fluorescence microscopy. Thanks to this method, the capability of PHA production by new haloarchaea isolates could be easily monitored.

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