The micro RNA hsa-miR-377-3p inhibits tumor growth in malignant melanoma

Malignant melanoma is one of the most aggressive skin cancers, having a very high mortality rate. However, its effective treatment is not clear. Ampelopsin, a plant flavonoid, has been reported to inhibit cell growth and/or induce apoptosis in various types of tumor. In this study, it was shown that ampelopsin significantly inhibits melanoma A375 cell line proliferation in a concentration-dependent/time-dependent manner. The flow cytometric data clearly demonstrated that ampelopsin causes cell cycle arrest in the G2/M phase. Moreover, it also confirmed that growth inhibition mediated by treatment with ampelopsin is related to the decreased expression of Cdc2, Cdc25c, cyclin B1, and activation of caspase-3 and Bax, purportedly by epidermal growth factor receptor (EGFR), extracellular regulated protein kinases, and matrix metalloproteinase-2 (MMP-2) downregulation. As a result of this work, these findings suggest that ampelopsin inhibits human malignant melanoma A375 cell line proliferation by suppressing the EGFR/ERK/MMP-2 pathway.

Download Full-text

Pharmacological and biochemical characterization of adenosine receptors in the human malignant melanoma A375 cell line

British Journal of Pharmacology ◽

10.1038/sj.bjp.0704352 ◽

2001 ◽

Vol 134 (6) ◽

pp. 1215-1226 ◽

Cited By ~ 82

Author(s):

Stefania Merighi ◽

Katia Varani ◽

Stefania Gessi ◽

Elena Cattabriga ◽

Valeria Iannotta ◽

...

Keyword(s):

Malignant Melanoma ◽

Cell Line ◽

Adenosine Receptors ◽

Biochemical Characterization ◽

Human Malignant Melanoma ◽

A375 Cell ◽

Melanoma A375 Cell ◽

A375 Cell Line

Download Full-text

In vitro evaluation of the anti-melanoma effects (A375 cell line) of the gel and whole leaf extracts from selected aloe species

Journal of Herbal Medicine ◽

10.1016/j.hermed.2022.100539 ◽

2022 ◽

pp. 100539

Author(s):

Alex Laux ◽

Josias Hamman ◽

Hanna Svitina ◽

Krzysztof Wrzesinski ◽

Chrisna Gouws

Keyword(s):

Cell Line ◽

Leaf Extracts ◽

In Vitro Evaluation ◽

A375 Cell ◽

A375 Cell Line

Download Full-text

High‐dose pyridoxal supplemented culture medium inhibits the growth of a human malignant melanoma cell line

Nutrition and Cancer ◽

10.1080/01635588309513773 ◽

1983 ◽

Vol 5 (1) ◽

pp. 10-15 ◽

Cited By ~ 20

Author(s):

Dennis M. DiSorbo ◽

Larry Nathanson

Keyword(s):

Malignant Melanoma ◽

Cell Line ◽

Melanoma Cell ◽

Melanoma Cell Line ◽

Culture Medium ◽

High Dose ◽

Human Malignant Melanoma ◽

Malignant Melanoma Cell

Download Full-text

Vitamin D and its low calcemic analogs modulate the anticancer properties of cisplatin and dacarbazine in the human melanoma A375 cell line

International Journal of Oncology ◽

10.3892/ijo.2019.4725 ◽

2019 ◽

Cited By ~ 1

Author(s):

Anna Piotrowska ◽

Justyna Wierzbicka ◽

Agnieszka Rybarczyk ◽

Robert Tuckey ◽

Andrzej Slominski ◽

...

Keyword(s):

Vitamin D ◽

Cell Line ◽

Human Melanoma ◽

Anticancer Properties ◽

A375 Cell ◽

Melanoma A375 Cell ◽

A375 Cell Line

Download Full-text

EGCG enhances TRAIL-mediated apoptosis in human melanoma A375 cell line

Journal of Huazhong University of Science and Technology [Medical Sciences] ◽

10.1007/s11596-009-0620-4 ◽

2009 ◽

Vol 29 (6) ◽

pp. 771-775 ◽

Cited By ~ 14

Author(s):

Qin Shen ◽

Fen Tian ◽

Ping Jiang ◽

Yanqiu Li ◽

Li Zhang ◽

...

Keyword(s):

Cell Line ◽

Human Melanoma ◽

A375 Cell ◽

Melanoma A375 Cell ◽

A375 Cell Line

Download Full-text

Cinobufagin Suppresses Melanoma Cell Growth by Inhibiting LEF1

International Journal of Molecular Sciences ◽

10.3390/ijms21186706 ◽

2020 ◽

Vol 21 (18) ◽

pp. 6706

Author(s):

Geon-Hee Kim ◽

Xue-Quan Fang ◽

Woo-Jin Lim ◽

Jooho Park ◽

Tae-Bong Kang ◽

...

Keyword(s):

Lung Cancer ◽

Cell Growth ◽

Tumor Growth ◽

Cancer Cells ◽

Melanoma Cell ◽

Transcriptional Activity ◽

Melanoma Cells ◽

Lung Cancer Cells ◽

Luciferase Assay ◽

Dependent Manner

Constitutive activation of the β-catenin dependent canonical Wnt signaling pathway, which enhances tumor growth and progression in multiple types of cancer, is commonly observed in melanoma. LEF1 activates β-catenin/TCF4 transcriptional activity, promoting tumor growth and progression. Although several reports have shown that LEF1 is highly expressed in melanoma, the functional role of LEF1 in melanoma growth is not fully understood. While A375, A2058, and G361 melanoma cells exhibit abnormally high LEF1 expression, lung cancer cells express lower LEF1 levels. A luciferase assay-based high throughput screening (HTS) with a natural compound library showed that cinobufagin suppressed β-catenin/TCF4 transcriptional activity by inhibiting LEF1 expression. Cinobufagin decreases LEF1 expression in a dose-dependent manner and Wnt/β-catenin target genes such as Axin-2, cyclin D1, and c-Myc in melanoma cell lines. Cinobufagin sensitively attenuates cell viability and induces apoptosis in LEF1 expressing melanoma cells compared to LEF1-low expressing lung cancer cells. In addition, ectopic LEF1 expression is sufficient to attenuate cinobufagin-induced apoptosis and cell growth retardation in melanoma cells. Thus, we suggest that cinobufagin is a potential anti-melanoma drug that suppresses tumor-promoting Wnt/β-catenin signaling via LEF1 inhibition.

Download Full-text