scholarly journals Customized hydrogel substrates for serum-free expansion of functional hMSCs

2020 ◽  
Vol 8 (14) ◽  
pp. 3819-3829
Author(s):  
Ngoc Nhi T. Le ◽  
Tianran Leona Liu ◽  
James Johnston ◽  
John D. Krutty ◽  
Kayla Marie Templeton ◽  
...  
Keyword(s):  
Cell Culture ◽  
Design Of Experiments ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Free Expansion ◽  
Serum Free ◽  
Synthetic Hydrogel ◽  
Stromal Cell Culture

Synthetic hydrogel arrays combined with a design of experiments approach identified hydrogel compositions for media-agnostic human mesenchymal stromal cell culture.

Increasing efficiency of human mesenchymal stromal cell culture by optimization of microcarrier concentration and design of medium feed

Cytotherapy ◽  
2015 ◽  
Vol 17 (2) ◽  
pp. 163-173 ◽  
Author(s):  
Allen Kuan-Liang Chen ◽  
Yi Kong Chew ◽  
Hong Yu Tan ◽  
Shaul Reuveny ◽  
Steve Kah Weng Oh
Keyword(s):  
Cell Culture ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Stromal Cell Culture

A xeno-free culture method that enhances Wharton's jelly mesenchymal stromal cell culture efficiency over traditional animal serum–supplemented cultures

Cytotherapy ◽  
2014 ◽  
Vol 16 (5) ◽  
pp. 683-691 ◽  
Author(s):  
Suphakde Julavijitphong ◽  
Suparat Wichitwiengrat ◽  
Nednapis Tirawanchai ◽  
Pornpimol Ruangvutilert ◽  
Chanchai Vantanasiri ◽  
...  
Keyword(s):  
Cell Culture ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Culture Method ◽  
Wharton’S Jelly ◽  
Wharton's Jelly ◽  
Stromal Cell Culture

scholarly journals Octanoate in Human Albumin Preparations Is Detrimental to Mesenchymal Stromal Cell Culture

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Way-Wua Wong ◽  
Andrew D. MacKenzie ◽  
Vicky J. Nelson ◽  
James M. Faed ◽  
Paul R. Turner
Keyword(s):  
Cell Culture ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Ex Vivo ◽  
Population Doubling ◽  
Growth Media ◽  
Stromal Cell Culture

Cell therapies hold great promise as the next major advance in medical treatment. To enable safe, effectiveex vivoculture whilst maintaining cell phenotype, growth media constituents must be carefully controlled. We have used a chemically defined mesenchymal stromal cell culture medium to investigate the influence of different preparations of human serum albumin. We examined two aspects of cell culture, growth rate as measured by population doubling time and colony forming ability which is a representative measure of the stemness of the cell population. Albumin preparations showed comparative differences in both of these criteria. Analysis of the albumin bound fatty acids also showed differences depending on the manufacturing procedure used. We demonstrated that octanoate, an additive used to stabilize albumin during pasteurization, slows growth and lowers colony forming ability duringex vivoculture. Further to this we also found the level of Na+/K+ATPase, a membrane bound cation pump inhibited by octanoate, is increased in cells exposed to this compound. We conclude that the inclusion of human serum albumin inex vivogrowth media requires careful consideration of not only the source of albumin, but also the associated molecular cargo, for optimal cell growth and behavior.

scholarly journals Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications

10.3791/54845 ◽  
2016 ◽  
Author(s):  
Luisa Trombi ◽  
Serena Danti ◽  
Sara Savelli ◽  
Stefania Moscato ◽  
Delfo D'Alessandro ◽  
...  
Keyword(s):  
Cell Culture ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Stromal Cell Culture ◽  
Orthopedic Applications

scholarly journals The possibilities of Cell-IQ phase-contrast microscopy for a continuous real-time observation of multipotent mesenchymal stromal cell culture

2018 ◽  
Vol 74 (3) ◽  
Author(s):  
Elena Sergeevna Melashchenko ◽  
Valeria Vladimirovna Shupletsova ◽  
Olga Gennadevna Khaziakhmatova ◽  
Vladimir Vladimirovich Malashchenko ◽  
Kristina Alekseevna Yurova ◽  
...  
Keyword(s):  
Cell Culture ◽  
Mesenchymal Stromal Cell ◽  
Phase Contrast ◽  
Stromal Cell ◽  
Phase Contrast Microscopy ◽  
Multipotent Mesenchymal Stromal Cell ◽  
Contrast Microscopy ◽  
Stromal Cell Culture ◽  
Time Observation ◽  
Real Time Observation

Stimulatory effect of ipriflavone on formation of bone-like tissue in rat bone marrow stromal cell culture

1992 ◽  
Vol 51 (S1) ◽  
pp. S16-S20 ◽  
Author(s):  
Kohei Notoya ◽  
Ryoichi Tsukuda ◽  
Keiji Yoshida ◽  
Shigehisa Taketomi
Keyword(s):  
Cell Culture ◽  
Bone Marrow ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Marrow Stromal Cell ◽  
Stromal Cell Culture ◽  
Rat Bone
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