Near-infrared ratiometric self-assembled theranostic nanoprobe: imaging and tracking cancer chemotherapy

2020 ◽  
Vol 56 (25) ◽  
pp. 3629-3632 ◽  
Author(s):  
Xinwei Tian ◽  
Zhao Li ◽  
Ning Ding ◽  
Jiahang Zhang
Keyword(s):  
Cancer Therapy ◽  
Real Time ◽  
Cancer Chemotherapy ◽  
Near Infrared ◽  
Self Assembled

A novel near-infrared ratiometric fluorescent theranostic nanoprobe is applied for real-time fluorescence tracking and imaging cancer therapy in vivo and in situ.

Azo-based near-infrared fluorescent theranostic probe for tracking hypoxia-activated cancer chemotherapy in vivo

2019 ◽  
Vol 55 (87) ◽  
pp. 13172-13175 ◽  
Author(s):  
Ning Ding ◽  
Zhao Li ◽  
Xinwei Tian ◽  
Jiahang Zhang ◽  
Kaili Guo ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Real Time ◽  
Cancer Chemotherapy ◽  
Near Infrared ◽  
Real Time Visualization

A novel azo-based near-infrared fluorescent therabostic probe activated by hypoxia is applied to real-time visualization of drug delivery in vivo.

scholarly journals Real-time observation of tetrapyrrole binding to an engineered bacterial phytochrome

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yusaku Hontani ◽  
Mikhail Baloban ◽  
Francisco Velazquez Escobar ◽  
Swetta A. Jansen ◽  
Daria M. Shcherbakova ◽  
...  
Keyword(s):  
Real Time ◽  
Rational Design ◽  
Near Infrared ◽  
Fluorescent Proteins ◽  
Covalent Bond ◽  
Binding Pocket ◽  
Tissue Imaging ◽  
Covalent Attachment ◽  
Time Resolved

AbstractNear-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes are widely used for structural and functional deep-tissue imaging in vivo. To fluoresce, NIR FPs covalently bind a chromophore, such as biliverdin IXa tetrapyrrole. The efficiency of biliverdin binding directly affects the fluorescence properties, rendering understanding of its molecular mechanism of major importance. miRFP proteins constitute a family of bright monomeric NIR FPs that comprise a Per-ARNT-Sim (PAS) and cGMP-specific phosphodiesterases - Adenylyl cyclases - FhlA (GAF) domain. Here, we structurally analyze biliverdin binding to miRFPs in real time using time-resolved stimulated Raman spectroscopy and quantum mechanics/molecular mechanics (QM/MM) calculations. Biliverdin undergoes isomerization, localization to its binding pocket, and pyrrolenine nitrogen protonation in <1 min, followed by hydrogen bond rearrangement in ~2 min. The covalent attachment to a cysteine in the GAF domain was detected in 4.3 min and 19 min in miRFP670 and its C20A mutant, respectively. In miRFP670, a second C–S covalent bond formation to a cysteine in the PAS domain occurred in 14 min, providing a rigid tetrapyrrole structure with high brightness. Our findings provide insights for the rational design of NIR FPs and a novel method to assess cofactor binding to light-sensitive proteins.

Hybrid MPI-MRI System for Dual-Modal In Situ Cardiovascular Assessments of Real-Time 3D Blood Flow Quantification—A Pre-Clinical In Vivo Feasibility Investigation

2020 ◽  
Vol 39 (12) ◽  
pp. 4335-4345
Author(s):  
Jochen Franke ◽  
Nicoleta Baxan ◽  
Heinrich Lehr ◽  
Ulrich Heinen ◽  
Sebastian Reinartz ◽  
...  
Keyword(s):  
Blood Flow ◽  
Real Time ◽  
Flow Quantification ◽  
Blood Flow Quantification

Near-Infrared Off-Axis Integrated Cavity Output Spectroscopic Gas Sensor for Real-Time, In Situ Atmospheric Methane Monitoring

2020 ◽  
pp. 1-1
Author(s):  
Kaiyuan Zheng ◽  
Chuantao Zheng ◽  
Haipeng Zhang ◽  
Junhao Li ◽  
Zidi Liu ◽  
...  
Keyword(s):  
Real Time ◽  
Gas Sensor ◽  
Near Infrared ◽  
Atmospheric Methane ◽  
Cavity Output

scholarly journals An amphiphilic dendrimer as a light-activable immunological adjuvant for in situ cancer vaccination

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yongchao Wang ◽  
Ningqiang Gong ◽  
Chi Ma ◽  
Yuxuan Zhang ◽  
Hong Tan ◽  
...  
Keyword(s):  
Near Infrared ◽  
Infrared Light ◽  
Immune Memory ◽  
Toll Like Receptor ◽  
Tumour Site ◽  
Adjuvant Effect ◽  
Cancer Vaccination ◽  
Tumour Metastasis

AbstractImmunological adjuvants are essential for successful cancer vaccination. However, traditional adjuvants have some limitations, such as lack of controllability and induction of systemic toxicity, which restrict their broad application. Here, we present a light-activable immunological adjuvant (LIA), which is composed of a hypoxia-responsive amphiphilic dendrimer nanoparticle loaded with chlorin e6. Under irradiation with near-infrared light, the LIA not only induces tumour cell lysis and tumour antigen release, but also promotes the structural transformation of 2-nitroimidazole containing dendrimer to 2-aminoimidazole containing dendrimer which can activate dendritic cells via the Toll-like receptor 7-mediated signaling pathway. The LIA efficiently inhibits both primary and abscopal tumour growth and induces strong antigen-specific immune memory effect to prevent tumour metastasis and recurrence in vivo. Furthermore, LIA localizes the immunological adjuvant effect at the tumour site. We demonstrate this light-activable immunological adjuvant offers a safe and potent platform for in situ cancer vaccination.

scholarly journals Boosting Chemodynamic Therapy by the Synergistic Effect of Co-Catalyze and Photothermal Effect Triggered by the Second Near-Infrared Light

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Songtao Zhang ◽  
Longhai Jin ◽  
Jianhua Liu ◽  
Yang Liu ◽  
Tianqi Zhang ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Bovine Serum Albumin ◽  
Synergistic Effect ◽  
Bovine Serum ◽  
Photothermal Therapy ◽  
Near Infrared ◽  
Infrared Light ◽  
Photothermal Effect ◽  
Reaction Efficiency

AbstractIn spite of the tumor microenvironments responsive cancer therapy based on Fenton reaction (i.e., chemodynamic therapy, CDT) has been attracted more attentions in recent years, the limited Fenton reaction efficiency is the important obstacle to further application in clinic. Herein, we synthesized novel FeO/MoS2 nanocomposites modified by bovine serum albumin (FeO/MoS2-BSA) with boosted Fenton reaction efficiency by the synergistic effect of co-catalyze and photothermal effect of MoS2 nanosheets triggered by the second near-infrared (NIR II) light. In the tumor microenvironments, the MoS2 nanosheets not only can accelerate the conversion of Fe3+ ions to Fe2+ ions by Mo4+ ions on their surface to improve Fenton reaction efficiency, but also endow FeO/MoS2-BSA with good photothermal performances for photothermal-enhanced CDT and photothermal therapy (PTT). Consequently, benefiting from the synergetic-enhanced CDT/PTT, the tumors are eradicated completely in vivo. This work provides innovative synergistic strategy for constructing nanocomposites for highly efficient CDT.

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