Azo-based near-infrared fluorescent theranostic probe for tracking hypoxia-activated cancer chemotherapy in vivo

A novel near-infrared ratiometric fluorescent theranostic nanoprobe is applied for real-time fluorescence tracking and imaging cancer therapy in vivo and in situ.

Download Full-text

Real-Time Visualization and Quantitation of Vascular Permeability In Vivo: Implications for Drug Delivery

PLoS ONE ◽

10.1371/journal.pone.0033760 ◽

2012 ◽

Vol 7 (3) ◽

pp. e33760 ◽

Cited By ~ 34

Author(s):

Desmond B. S. Pink ◽

Wendy Schulte ◽

Missag H. Parseghian ◽

Andries Zijlstra ◽

John D. Lewis

Keyword(s):

Drug Delivery ◽

Vascular Permeability ◽

Real Time ◽

Real Time Visualization

Download Full-text

Real-time observation of tetrapyrrole binding to an engineered bacterial phytochrome

Communications Chemistry ◽

10.1038/s42004-020-00437-3 ◽

2021 ◽

Vol 4 (1) ◽

Author(s):

Yusaku Hontani ◽

Mikhail Baloban ◽

Francisco Velazquez Escobar ◽

Swetta A. Jansen ◽

Daria M. Shcherbakova ◽

...

Keyword(s):

Real Time ◽

Rational Design ◽

Near Infrared ◽

Fluorescent Proteins ◽

Covalent Bond ◽

Binding Pocket ◽

Tissue Imaging ◽

Covalent Attachment ◽

Time Resolved

AbstractNear-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes are widely used for structural and functional deep-tissue imaging in vivo. To fluoresce, NIR FPs covalently bind a chromophore, such as biliverdin IXa tetrapyrrole. The efficiency of biliverdin binding directly affects the fluorescence properties, rendering understanding of its molecular mechanism of major importance. miRFP proteins constitute a family of bright monomeric NIR FPs that comprise a Per-ARNT-Sim (PAS) and cGMP-specific phosphodiesterases - Adenylyl cyclases - FhlA (GAF) domain. Here, we structurally analyze biliverdin binding to miRFPs in real time using time-resolved stimulated Raman spectroscopy and quantum mechanics/molecular mechanics (QM/MM) calculations. Biliverdin undergoes isomerization, localization to its binding pocket, and pyrrolenine nitrogen protonation in <1 min, followed by hydrogen bond rearrangement in ~2 min. The covalent attachment to a cysteine in the GAF domain was detected in 4.3 min and 19 min in miRFP670 and its C20A mutant, respectively. In miRFP670, a second C–S covalent bond formation to a cysteine in the PAS domain occurred in 14 min, providing a rigid tetrapyrrole structure with high brightness. Our findings provide insights for the rational design of NIR FPs and a novel method to assess cofactor binding to light-sensitive proteins.

Download Full-text

Near-infrared persistent luminescence hollow mesoporous nanospheres for drug delivery and in vivo renewable imaging

Journal of Materials Chemistry B ◽

10.1039/c6tb02674e ◽

2016 ◽

Vol 4 (48) ◽

pp. 7845-7851 ◽

Cited By ~ 18

Author(s):

Junpeng Shi ◽

Meng Sun ◽

Xia Sun ◽

Hongwu Zhang

Keyword(s):

Drug Delivery ◽

Near Infrared ◽

High Sensitivity ◽

Drug Carriers ◽

Template Method ◽

Persistent Luminescence ◽

Deep Tissue ◽

Large Capacity

Near-infrared persistent luminescence hollow mesoporous nanospheres have been synthesized via a template method. These nanospheres can be used as large capacity drug carriers and realize super long-term and high sensitivity tracking of drug delivery in deep tissue.

Download Full-text

Non-invasive, real-time reporting drug release in vitro and in vivo

Chemical Communications ◽

10.1039/c4cc09920f ◽

2015 ◽

Vol 51 (32) ◽

pp. 6948-6951 ◽

Cited By ~ 31

Author(s):

Yanfeng Zhang ◽

Qian Yin ◽

Jonathan Yen ◽

Joanne Li ◽

Hanze Ying ◽

...

Keyword(s):

Drug Release ◽

Real Time ◽

Near Infrared ◽

Reporting System ◽

Real Time Monitoring ◽

Near Infrared Fluorescence ◽

Non Invasive ◽

Fluorescence Dye

Anin vitroandin vivodrug-reporting system is developed for real-time monitoring of drug release via the analysis of the concurrently released near-infrared fluorescence dye.

Download Full-text

Novel concept of the smart NIR-light–controlled drug release of black phosphorus nanostructure for cancer therapy

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1714421115 ◽

2018 ◽

Vol 115 (3) ◽

pp. 501-506 ◽

Cited By ~ 356

Author(s):

Meng Qiu ◽

Dou Wang ◽

Weiyuan Liang ◽

Liping Liu ◽

Yin Zhang ◽

...

Keyword(s):

Drug Delivery ◽

Cancer Therapy ◽

Drug Release ◽

Drug Delivery System ◽

Delivery System ◽

Near Infrared ◽

Black Phosphorus ◽

Deep Penetration ◽

Nir Light

A biodegradable drug delivery system (DDS) is one the most promising therapeutic strategies for cancer therapy. Here, we propose a unique concept of light activation of black phosphorus (BP) at hydrogel nanostructures for cancer therapy. A photosensitizer converts light into heat that softens and melts drug-loaded hydrogel-based nanostructures. Drug release rates can be accurately controlled by light intensity, exposure duration, BP concentration, and hydrogel composition. Owing to sufficiently deep penetration of near-infrared (NIR) light through tissues, our BP-based system shows high therapeutic efficacy for treatment of s.c. cancers. Importantly, our drug delivery system is completely harmless and degradable in vivo. Together, our work proposes a unique concept for precision cancer therapy by external light excitation to release cancer drugs. If these findings are successfully translated into the clinic, millions of patients with cancer will benefit from our work.

Download Full-text

A reversible near-infrared fluorescence probe for the monitoring of HSO3–/H2O2 regulated cycles in vivo

New Journal of Chemistry ◽

10.1039/d1nj03507j ◽

2021 ◽

Author(s):

Qiuying Song ◽

Bo Zhou ◽

Dongyu Zhang ◽

Haijun Chi ◽

Hongmin Jia ◽

...

Keyword(s):

Real Time ◽

Near Infrared ◽

Fluorescence Probe ◽

Redox Homeostasis ◽

Research Field ◽

Fluorescence Probes ◽

Detection Capability ◽

Near Infrared Fluorescence ◽

Real Time Detection

The development of well-designed fluorescence probes for the monitoring redox homeostasis in biosystems has become a desired research field owing to their noninvasive and real-time detection capability in vivo. In...

Download Full-text

A near-infrared reversible fluorescent probe for real-time imaging of redox status changes in vivo

Chemical Science ◽

10.1039/c2sc22076h ◽

2013 ◽

Vol 4 (3) ◽

pp. 1079 ◽

Cited By ~ 142

Author(s):

Kehua Xu ◽

Mingming Qiang ◽

Wen Gao ◽

Ruixian Su ◽

Na Li ◽

...

Keyword(s):

Real Time ◽

Fluorescent Probe ◽

Near Infrared ◽

Redox Status ◽

Real Time Imaging

Download Full-text

NIR-Responsive Lysosomotropic Phototrigger: ʽAIE + ESIPTʼ Active Naphthalene Based Single Component Photoresponsive Nanocarrier with Two-Photon Uncaging and Real-Time Monitoring Ability

10.33774/chemrxiv-2021-nftrb ◽

2021 ◽

Author(s):

Biswajit Roy ◽

Rakesh Mengji ◽

Samrat Roy ◽

Bipul Pal ◽

Avijit Jana ◽

...

Keyword(s):

Drug Delivery ◽

Drug Release ◽

Real Time ◽

Near Infrared ◽

Single Component ◽

Photon Absorption ◽

Real Time Monitoring ◽

Two Photon ◽

Nir Light

In recent times, organelle-targeted drug delivery systems gained tremendous attention due to the site specific delivery of active drug molecules resulting in enhanced bioefficacy. In this context, the phototriggered drug delivery system (DDS) for releasing an active molecule is superior as it provides spatial and temporal control over the release. So far, near infrared (NIR) light responsive organelle targeted DDS has not yet been developed. Hence, we introduced a two-photon NIR-light responsive lysosome targeted ʽAIE + ESIPTʼ active single component DDS based on naphthalene chromophore. The Two-photon absorption cross-section of our DDS is 142 GM at 850 nm. The DDS was converted into pure organic nanoparticles for biological applications. Our nano-DDS is capable of selective targeting, AIE-luminogenic imaging, and drug release within the lysosome. In vitro studies using cancerous cell lines showed that our single component photoresponsive nanocarrier exhibited enhanced cytotoxicity and real-time monitoring ability of the drug release.

Download Full-text

Multiplexed Non-invasive in vivo Imaging to Assess Metabolism and Receptor Engagement in Tumor Xenografts

10.1101/758383 ◽

2019 ◽

Author(s):

Alena Rudkouskaya ◽

Nattawut Sinsuebphon ◽

Marien Ochoa ◽

Joe E. Mazurkiewicz ◽

Xavier Intes ◽

...

Keyword(s):

Drug Delivery ◽

Targeted Delivery ◽

Near Infrared ◽

Metabolic Response ◽

Imaging Modalities ◽

Wide Field ◽

Receptor Ligand ◽

Non Invasive ◽

Intracellular Drug Delivery

AbstractFollowing an ever-increased focus on personalized medicine, there is a continuing need to develop preclinical molecular imaging modalities to guide the development and optimization of targeted therapies. To date, non-invasive quantitative imaging modalities that can comprehensively assess simultaneous cellular drug delivery efficacy and therapeutic response are lacking. In this regard, Near-Infrared (NIR) Macroscopic Fluorescence Lifetime Förster Resonance Energy Transfer (MFLI-FRET) imaging offers a unique method to robustly quantify receptor-ligand engagement in vivo and subsequent intracellular internalization, which is critical to assess the delivery efficacy of targeted therapeutics. However, implementation of multiplexing optical imaging with FRET in vivo is challenging to achieve due to spectral crowding and cross-contamination. Herein, we report on a strategy that relies on a dark quencher that enables simultaneous assessment of receptor-ligand engagement and tumor metabolism in intact live mice. First, we establish that IRDye QC-1 (QC-1) is an effective NIR dark acceptor for the FRET-induced quenching of donor Alexa Fluor 700 (AF700) using in vitro NIR FLI microscopy and in vivo wide-field MFLI imaging. Second, we report on simultaneous in vivo imaging of the metabolic probe IRDye 800CW 2-deoxyglucose (2-DG) and MFLI-FRET imaging of NIR-labeled transferrin FRET pair (Tf-AF700/Tf-QC-1) uptake in tumors. Such multiplexed imaging revealed an inverse relationship between 2-DG uptake and Tf intracellular delivery, suggesting that 2-DG signal may predict the efficacy of intracellular targeted delivery. Overall, our methodology enables for the first time simultaneous non-invasive monitoring of intracellular drug delivery and metabolic response in preclinical studies.

Download Full-text